We narrowed to 20,231 results for: ATO

1. mActA-mSspB-mCherry

   Plasmid

   #210294

   Purpose

   ActuAtor for iLID optogenetics

   Depositor

   Takanari Inoue

   Article

   Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

   Insert

   mActA-,SspB-mCherry
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTol1 - 14xUAS:CaSR-EGFP

   Plasmid

   #191502

   Purpose

   A UAS construct used to create transgenic fish that express CaSR-EGFP when crossed to a fish carrying a Gal4.

   Depositor

   Michael Granato

   Article

   Shoenhard et al Cell Rep. 2022 Dec 6;41(10):111790. doi:

   Insert

   CaSR-EGFP-SV40
   

   Use

   Creation of transgenic fish using tol1 transgenes…

   Promoter

   14X UAS

   Available Since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. RNF149 shRNA-TRE

   Plasmid

   #225337

   Purpose

   shRNAmir backbone under tet-operator for RNA interference, with YFP

   Depositor

   Huda Zoghbi

   Article

   Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

   Insert

   RNF149 shRNA (Rnf149 Mouse)
   

   Use

   AAV and RNAi

   Expression

   Mammalian

   Promoter

   TRE (tetracycline-responsive element)

   Available Since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. USP7 shRNA-TRE

   Plasmid

   #225338

   Purpose

   shRNAmir backbone under tet-operator for RNA interference, with YFP

   Depositor

   Huda Zoghbi

   Article

   Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

   Insert

   USP7 shRNA (Usp7 Mouse)
   

   Use

   AAV and RNAi

   Expression

   Mammalian

   Promoter

   TRE (tetracycline-responsive element)

   Available Since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Luciferase shRNA-TRE

   Plasmid

   #225339

   Purpose

   shRNAmir backbone under tet-operator for RNA interference, with YFP

   Depositor

   Huda Zoghbi

   Article

   Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

   Insert

   Luciferase shRNA (LOC116160065 Mouse)
   

   Use

   AAV and RNAi

   Expression

   Mammalian

   Promoter

   TRE (tetracycline-responsive element)

   Available Since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXS-IRES-Blast coUCK2 D62A

   Plasmid

   #211530

   Purpose

   Expresses codon optimized catalytic mutant UCK2

   Depositor

   Richard Possemato

   Article

   Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

   Insert

   UCK2 D62A (UCK2 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   D62A (catalytic mutant)

   Available Since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-N2-IFT20

   Plasmid

   #218719

   Purpose

   Expresses C-terminally EGFP-tagged IFT20 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

   Insert

   IFT20 (IFT20 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-C1-BBS9

   Plasmid

   #218717

   Purpose

   Expresses N-terminally EGFP-tagged BBS9 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

   Insert

   BBS9 (BBS9 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   A455T (g1876a)

   Promoter

   CMV

   Available Since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCPH2A.Z-GS3

   Plasmid

   #204758

   Purpose

   Expression of Cas9 and human H2A.Z

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Insert

   H2AZ1 (H2AZ1 Human)
   

   Expression

   Mammalian

   Available Since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CMV-dSa VP64 Hbb

    Plasmid

    #99693

    Purpose

    Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Hbb promoter, vector allows for activation of mouse Hbb, can be packaged and delivered as AAV

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9 and gRNA targeting Hbb (gRNA: GGGGTAAGGGGAGCAAGGTC) (Hbb Mouse, Synthetic)
    

    Use

    AAV and CRISPR

    Tags

    VP64

    Mutation

    dead Cas9

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-C1-IFT38

    Plasmid

    #218723

    Purpose

    Expresses N-terminally EGFP-tagged IFT38 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

    Insert

    IFT38 (CLUAP1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAf-CRISPR-ctfR1

    Plasmid

    #207992

    Purpose

    CRISPR vector used with pAf-CRISPR-phoA (#207991) to delete both aflatoxin and cyclopiazonic acid gene clusters of Aspergillus flavus

    Depositor

    Perng-Kuang Chang

    Article

    Chang et al Fungal Genet Biol. 2023 Dec 27;170:10386

    Insert

    ctfR1
    

    Use

    CRISPR

    Promoter

    Aspergillus flavus U6

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti_CHyMErA_U6(SpCas9_I-SceI)-(AsCas12_PacI)_PGK-puro

    Plasmid

    #189634

    Purpose

    Lentiviral expression of single SpCas9 and AsCas12a gRNAs for generating combinatorial CHyMErA 3Cs libraries

    Depositor

    Manuel Kaulich

    Article

    Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

    Insert

    hU6 Cas9-Cas12a gRNA cassette, PGK puro cassette
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pASPIre4

    Plasmid

    #196656

    Purpose

    Derivative of pASPIre3. Contains SpeI restriction site within the CDS of bxb1 to enable diversification of the 5’-UTR and codons 2-16.

    Depositor

    Markus Jeschek

    Article

    Hollerer et al Nucleic Acids Res. 2023 Mar 21;51(5):237

    Insert

    Bxb1-sfGFP fusion controlled by rhamnose promoter; attB/attP-flanked discriminator; exchangable 5'-UTR and CDS (codons 1-16)
    

    Expression

    Bacterial

    Mutation

    SpeI site in Bxb1 CDS

    Promoter

    rhamnose-inducible promoter

    Available Since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pXD70DA7-DadR/DadS-Pdadh-DadhABC(A2)

    Plasmid

    #191630

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain with transcriptional regulators DadR/DadS

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    Dopamine dehydroxylase
    

    Expression

    Bacterial

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pXD70Tet(DadHR)-DadR-Pdadh-DadhABC(A2)

    Plasmid

    #191645

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (TetR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain with transcriptional regulator DadR

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    Dopamine dehydroxylase
    

    Expression

    Bacterial

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDF0428 huDisCas7-11-R1045-GGGS-R1122-U6-pro-Gluc

    Plasmid

    #186985

    Purpose

    AAV transgene plasmid for Cas7-11-R1045-GGGS-R1122, with U6 promoter-driven expression of Gluc crRNA guide

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

    Insert

    cas7-11-r1045-gggs-r1122, Gluc crRNA guide
    

    Use

    AAV

    Mutation

    r1045-gggs-r1122 deletion

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti6.3delta4 GPR3-V5

    Plasmid

    #172601

    Purpose

    Lentiviral plasmid expressing c-terminal V5-tagged GPR3

    Depositor

    Robert Screaton

    Article

    Iorio et al Commun Biol. 2021 Jul 23;4(1):907. doi:

    Insert

    G protein-Coupled Receptor 3 (GPR3 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. CoxVIIIx2-CeNL(Ca2+)_1.2μ-pcDNA3

    Plasmid

    #111922

    Purpose

    Cyan color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of CeNL(Ca2+)_1.2μ

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    CeNL(Ca2+)_1.2μ
    

    Tags

    CoxVIIIx2

    Expression

    Mammalian

    Mutation

    E67D, E104D, D133E at CaM

    Promoter

    CMV

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX459V2.0-eSpCas9(1.1)+K855A

    Plasmid

    #108298

    Purpose

    pX459 V2.0 (Plasmid #62988) with the K848A, K855A, K1003A, and R1060A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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