We narrowed to 23,934 results for: cel.2

1. pCDNA4 3xFLAG Halo delta 122-130 nsp1

   Plasmid

   #175425

   Purpose

   Expresses delta 122-130 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV2 delta 122-130 nsp1
   

   Use

   Tags

   3XFLAG Halo

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA4 3xFLAG Halo E55A E57A K58A nsp1

   Plasmid

   #175427

   Purpose

   Expresses E55A E57A K58A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   nsp1 E55A E57A K58A
   

   Use

   Tags

   3XFLAG Halo

   Expression

   Mammalian

   Mutation

   N-terminal mutants E55A E57A K58A

   Promoter

   CMV

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCW57.1-Tet-UPF1S124A/N138A/T139A (pRKB268)

   Plasmid

   #99144

   Purpose

   Inducible lentiviral construct to express FLAG-tagged UPF1S124A/N138A/T139A

   Depositor

   Robert Bradley

   Article

   Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

   Insert

   UPF1 (UPF1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   First 127 codons are optimized to reduce GC conte…

   Promoter

   pTight

   Available since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA3 Flag MKK7B2Jnk1a1

   Plasmid

   #19726

   Depositor

   Roger Davis

   Article

   Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

   Insert

   MKK7B2Jnk1a1 (Map2k7, MAPK8 Human, Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 24, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3 Flag Jnk1a1MKK7B2

   Plasmid

   #19731

   Depositor

   Roger Davis

   Article

   Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

   Insert

   Jnk1a1MKK7b2 (Map2k7, MAPK8 Human, Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 24, 2009

   Availability

   Academic Institutions and Nonprofits only

6. pIKM1

   Plasmid

   #51054

   Purpose

   Kanamycin and erethromycin resistance

   Depositor

   Juergen Wiegel, Lee Lynd

   Article

   Tyurin et al Appl Environ Microbiol. 2004 Feb;70(2):8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 19, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pET28-His-MBP-NLS-RfxCas13d-NLS-HA

   Plasmid

   #171586

   Purpose

   Bacterial expression of NLS-RfxCas13d-NLS-HA

   Depositor

   Neville Sanjana

   Article

   Mendez-Mancilla et al Cell Chem Biol. 2022 Feb 17;29(2):321-32

   Insert

   His-MBP-NLS-RfxCas13d-NLS-HA
   

   Use

   Tags

   HA

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. RCAS(A) dnFREK (CT#1036)

   Plasmid

   #13900

   Depositor

   Cliff Tabin

   Article

   Brent et al Cell. 2003 Apr 18. 113(2):235-48.

   Insert

   FREK
   

   Use

   Retroviral; Avian expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 16, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pKMyc

   Plasmid

   #19400

   Depositor

   Ian Macara

   Article

   Joberty et al Nat Cell Biol 2000 Aug;2(8):531-9.

   Type

   Empty backbone

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 2, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28-MKIEE-NLS-RfxCas13d-NLS-HA-6His

    Plasmid

    #171587

    Purpose

    Bacterial expression of NLS-RfxCas13d-NLS 6His

    Depositor

    Neville Sanjana

    Article

    Mendez-Mancilla et al Cell Chem Biol. 2022 Feb 17;29(2):321-32

    Insert

    MKIEE-NLS-RfxCas13d-NLS-HA-6His
    

    Use

    Tags

    HA-6HIS and MKIEE

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKERep

    Plasmid

    #211826

    Purpose

    Medium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKESa

    Plasmid

    #211828

    Purpose

    Low copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Feb. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. NpT7-5-Erk2

    Plasmid

    #39230

    Depositor

    Melanie Cobb

    Article

    Robbins et al J Biol Chem. 1993 Mar 5;268(7):5097-106.

    Insert

    Erk2 (Mapk1 Rat)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

14. LLP762_L2pV1_OR-OCS_TCTP-Flp_s35S-Cre

    Plasmid

    #192421

    Purpose

    To act as the 2-input state of the OR gate.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-loxP-OCS-FRT-loxP::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA FLAG TORC1 (1-630)

    Plasmid

    #22974

    Depositor

    Marc Montminy

    Article

    Conkright et al Mol Cell. 2003 Aug . 12(2):413-23.

    Insert

    TORC1 (1-630) (CRTC1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Amino acids 1-630 rather than 1-634.

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pQCXIN X2/pTER shEGFP#1 (w491-1)

    Plasmid

    #19167

    Purpose

    Retroviral eGFP shRNA #2 (H1/TO promoter), 3’LTR insertion, Neo

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    Enhanced green fluorescent protein shRNA #2
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF9c/14

    Plasmid

    #162820

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF9c/14

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_ORF9c/14
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    CoV2_ORF9c/14(Met1,Amber)

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b(CoV1h)

    Plasmid

    #162822

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b(CoV1h)

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_ORF3b(CoV1h)
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    CoV2_ORF3b(CoV1h)(Met1,Amber)

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBMN DHFR-HiBiT / Lyt2

    Plasmid

    #179731

    Purpose

    Expresses human dihydrofolate reductase (DHFR) with C-terminal HiBiT tag in mammalian cells. This retroviral vector has an IRES-Lyt-2 downstream of the cloning sites

    Depositor

    James Inglese

    Article

    Rana et al Cell Chem Biol. 2024 Feb 15;31(2):221-23

    Insert

    human DHFR-HiBiT and IRES-Lyt-2 (DHFR Mouse, Human)
    

    Use

    Retroviral

    Tags

    HiBiT

    Expression

    Mutation

    Promoter

    LTR/MMLV

    Available since

    Oct. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA4TO-3XFLAG Halo WT nsp1

    Plasmid

    #175412

    Purpose

    Expressing WT CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 nsp1 (ORF1ab CoV2)
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge