We narrowed to 4,893 results for: lenti sgrna

1. lenti sgRNA(MS2)_puro optimized backbone

   Plasmid

   #73797

   Purpose

   optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and EF1A

   Available since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti_sgRNA(MS2)_MCP-KRAB-IRES-zsGreen1

   Plasmid

   #138460

   Purpose

   lenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-KRAB

   Depositor

   Jian Xu

   Article

   Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

   Insert

   MCP-KRAB-IRES-zsGreen1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Oct. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. lentiCRISPRv2-sgRNAs-del-CDK5RAP2-SVA-BFP

   Plasmid

   #202824

   Purpose

   Lentiviral vector modified to express two sgRNAs that delete the intronic SVA within the CDK5RAP2 gene with EF-1apha for Cas9 and BFP expression

   Depositor

   Matthew Anderson

   Article

   Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

   Insert

   two sgRNAs (each with a U6 promoter) to delete SVA within CDK5RAP2 gene
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 - two

   Available since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti_sgRNA(MS2)_MCP-VP64-IRES-zsGreen1

   Plasmid

   #138461

   Purpose

   lenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-VP64

   Depositor

   Jian Xu

   Article

   Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

   Insert

   MCP-VP64-IRES-zsGreen1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   May 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. lenti-KRAS-G121C/G13A-nick sgRNA

   Plasmid

   #214099

   Purpose

   Lentiviral vector expressing nicking sgRNA to induce KRAS G12C or G13A mutation using PE3

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Insert

   KRAS G12C or G13A nicking sgRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. lentiCRISPRv2-sgRNAs-del-SCN8A-SVA-BFP

   Plasmid

   #202826

   Purpose

   Lentiviral vector modified to express two sgRNAs that delete the intronic SVA within the SCN8A gene with EF-1apha for Cas9 and BFP expression

   Depositor

   Matthew Anderson

   Article

   Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

   Insert

   two sgRNAs (each with a U6 promoter) to delete intronic SVA within SCN8A gene
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 - two

   Available since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. lentiCRISPRv2-sgRNAs-del-AK057321-SVA-mcherry

   Plasmid

   #202821

   Purpose

   Lentiviral vector modified to express two sgRNAs that delete the SVA within the SVA-lncRNA AK057321 gene (exon 3) with EF-1apha for Cas9 and mcherry expression

   Depositor

   Matthew Anderson

   Article

   Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

   Insert

   two sgRNAs (each with a U6 promoter) to delete SVA within SVA-lncRNA AK057321 gene
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 (two)

   Available since

   July 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiCRISPRv2-sgRNAs-del-CHAF1B-SVA-BFP

   Plasmid

   #202825

   Purpose

   Lentiviral vector modified to express two sgRNAs that delete the intronic SVA within the CHAF1B gene with EF-1apha for Cas9 and BFP expression

   Depositor

   Matthew Anderson

   Article

   Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

   Insert

   two sgRNAs (each with a U6 promoter) to delete intronic SVA within CHAF1B gene
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 - two

   Available since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti sgRNA NGFR GFP out of frame

   Plasmid

   #155282

   Purpose

   Lentiviral plasmid for sgRNA, NGFR marker, editing detected with GFP, used with 155280

   Depositor

   Martin Carroll

   Insert

   GFP out of frame IRES NGFR
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti sgRNA NGFR CFP out of frame

    Plasmid

    #155283

    Purpose

    Lentiviral plasmid for sgRNA, NGFR marker, editing detected with CFP, used with 155280

    Depositor

    Martin Carroll

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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