Skip to main content
Addgene
Showing: 321 - 340 of 1762 results

  1. pNSU299_LYS9

    Plasmid
    #166085
    Purpose
    Plasmid for constituive spCas9 and tet-inducible LYS9 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    LYS9 (LYS9 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166085-plasmid-map-sequence-id-329116

    Enlarge

  2. pCAS9id_TRP

    Plasmid
    #141254
    Purpose
    Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassettes with stuffer and Kpn restriction sites.
    Depositor
    Adam Bertl
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141254-plasmid-map-sequence-id-308894

    Enlarge

  3. pCAS9i_TRP

    Plasmid
    #141253
    Purpose
    Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141253-plasmid-map-sequence-id-308890

    Enlarge

  4. Peripherin/pAS2-1

    Plasmid
    #98135
    Purpose
    Peripherin (NM_006262) in DNA-binding domain (BD) vector, used for yeast-two hybrid
    Depositor
    Rita Shiang
    Insert
    Peripherin (PRPH Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 21, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    98135-plasmid-map-sequence-id-191140

    Enlarge

  5. pTH341-SUP45(139-437)-GalBD

    Plasmid
    #29741
    Depositor
    Tobias von der Haar
    Insert
    SUP45 (SUP45 Budding Yeast)
    Use
    Tags
    Gal Activation domain and Myc
    Expression
    Yeast
    Mutation
    Codons 139-437 of the yeast SUP45 gene only
    Promoter
    Available since
    Sept. 21, 2011
    Availability
    Academic Institutions and Nonprofits only
    29741-plasmid-map-sequence-id-13825

    Enlarge

  6. pTH339-SUP45(1-138)-GalBD

    Plasmid
    #29739
    Depositor
    Tobias von der Haar
    Insert
    SUP45 (SUP45 Budding Yeast)
    Use
    Tags
    Gal Activation domain and Myc
    Expression
    Yeast
    Mutation
    Codons 1-138 of the yeast SUP45 gene only
    Promoter
    Available since
    Sept. 13, 2011
    Availability
    Academic Institutions and Nonprofits only
    29739-plasmid-map-sequence-id-13823

    Enlarge

  7. pTH342-SUP45(276-437)-GalBD

    Plasmid
    #29742
    Depositor
    Tobias von der Haar
    Insert
    SUP45 (SUP45 Budding Yeast)
    Use
    Tags
    Gal Activation domain and Myc
    Expression
    Yeast
    Mutation
    Codons 276-437 of the yeast SUP45 gene only
    Promoter
    Available since
    Sept. 13, 2011
    Availability
    Academic Institutions and Nonprofits only
    29742-plasmid-map-sequence-id-13826

    Enlarge

  8. pTH340-SUP45(1-242)-GalBD

    Plasmid
    #29740
    Depositor
    Tobias von der Haar
    Insert
    SUP45 (SUP45 Budding Yeast)
    Use
    Tags
    Gal Activation domain and Myc
    Expression
    Yeast
    Mutation
    Codons 1-242 of the yeast SUP45 gene only
    Promoter
    Available since
    Sept. 13, 2011
    Availability
    Academic Institutions and Nonprofits only
    29740-plasmid-map-sequence-id-13824

    Enlarge

  9. p404GALL

    Plasmid
    #17418
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    GALL promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    GALL promoter is between SacI and XbaI restrictio…
    Promoter
    Available since
    March 12, 2008
    Availability
    Academic Institutions and Nonprofits only
    17418-plasmid-map-sequence-id-8593

    Enlarge

  10. pNSU299_MET16

    Plasmid
    #166091
    Purpose
    Plasmid for constituive spCas9 and tet-inducible MET16 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    MET16 (MET16 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    May 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166091-plasmid-map-sequence-id-329609

    Enlarge

  11. pNSU299_CPR1_1

    Plasmid
    #166074
    Purpose
    Plasmid for constituive spCas9 and tet-inducible CPR1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    CPR1 (CPR1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166074-plasmid-map-sequence-id-330357

    Enlarge

  12. pNSU299_RRT8

    Plasmid
    #166080
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RRT8 for double stranded break formation in yeast.
    Depositor
    Rebecca Burgess
    Insert
    Promoter of RRT8 (RRT8 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166080-plasmid-map-sequence-id-330356

    Enlarge

  13. pNSU299_CPR1_2

    Plasmid
    #166071
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of an sgRNA targeting an intergenic site near CPR1 for double stranded break formation in yeast.
    Depositor
    Rebecca Burgess
    Insert
    Intergenic region near CPR1
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166071-plasmid-map-sequence-id-330355

    Enlarge

  14. pNSU299_GAL7

    Plasmid
    #166089
    Purpose
    Plasmid for constituive spCas9 and tet-inducible GAL7 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    GAL7 (GAL7 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166089-plasmid-map-sequence-id-330353

    Enlarge

  15. pNSU299_GLK1

    Plasmid
    #166090
    Purpose
    Plasmid for constituive spCas9 and tet-inducible GLK1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    GLK1 (GLK1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    166090-plasmid-map-sequence-id-329612

    Enlarge

  16. pNSU299_LHS1

    Plasmid
    #166092
    Purpose
    Plasmid for constituive spCas9 and tet-inducible LHS1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    LHS1 (LHS1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    166092-plasmid-map-sequence-id-329611

    Enlarge

  17. pNSU299_RPL22A

    Plasmid
    #166079
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RPL22A for double stranded break formation in yeast.
    Depositor
    Rebecca Burgess
    Insert
    Promoter of RPL22A (RPL22A Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    166079-plasmid-map-sequence-id-329605

    Enlarge

  18. pNSU299_YBL055C

    Plasmid
    #166077
    Purpose
    Plasmid for constituive spCas9 and tet-inducible YBL055C targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    YBL055C (near PTC3) (YBL055C Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166077-plasmid-map-sequence-id-329132

    Enlarge

  19. pNSU299_YNR071C

    Plasmid
    #166081
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of YNR071C for double stranded break formation in yeast.
    Depositor
    Rebecca Burgess
    Insert
    Promoter of YNR071C (YNR071C Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166081-plasmid-map-sequence-id-329131

    Enlarge

  20. pNSU299_SLA1

    Plasmid
    #166075
    Purpose
    Plasmid for constituive spCas9 and tet-inducible SLA1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    SLA1 (SLA1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166075-plasmid-map-sequence-id-329130

    Enlarge

Showing: 321 - 340 of 1762 results