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We narrowed to 9,771 results for: crispr plasmids

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Showing: 321 - 340 of 9771 results

  1. Ir7f-T2A-QF2 HDR plasmid

    Plasmid
    #140942
    Purpose
    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Ir7f gene
    Depositor
    Leslie Vosshall
    Article
    Jove et al Neuron. 2020 Dec 23;108(6):1163-1180.e12
    Insert
    Ir7f-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir7f-right-HDR-arm
    Mutation
    4 point mutations (annotated on plasmid map) were…
    Available Since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    140942-plasmid-map-sequence-id-313600

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  2. fru-T2A-CsChrimson-tdTomato HDR plasmid

    Plasmid
    #141100
    Purpose
    Plasmid for CRISPR-generated knock-in line that expresses optogenetic activator CsChrimson-tdTomato by knocking-in fragment into the coding sequence of the endogenous Aedes aegypti fru gene
    Depositor
    Leslie Vosshall
    Article
    Basrur et al Elife. 2020 Dec 7;9. pii: 63982. doi: 10
    Insert
    fru-left-HDR-arm; T2A-CsChrimson-tdTomato-SV40; 3xP3-EYFP-SV40; fru-right-HDR-arm
    Use
    Donor template
    Available Since
    Aug. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    141100-plasmid-map-sequence-id-292288

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  9. PB-Puro-U6-gRNA-pA plasmid

    Plasmid
    #247671
    Purpose
    spCas9 gRNA is driven by human U6 promoter, with the gRNA cassette between PuroR and pA, making it detectable in polyA-based RNA-seq.
    Depositor
    Tae Hyun Kim
    Insert
    Puromycin resistant gene
    Expression
    Mammalian
    Promoter
    TK promoter
    Available Since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    247671-plasmid-map-sequence-id-491686

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  12. Lentiviral click editor (CE1) plasmid - LTR-pEF1a-PCV2-nCas9-EcKlenow (DRR921)

    Plasmid
    #217798
    Purpose
    Lentiviral plasmid to produce the prototypical CE1 construct (PCV2-nCas9-EcKlenow), expressed from the EF1a promoter
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR and Lentiviral
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    EF1a
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217798-plasmid-map-sequence-id-432865

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  13. pCut plasmid toolkit

    Plasmid Kit
    #1000000118
    Purpose
    Cas9-based kit in S. cerevisiae for optimizing heterologous gene expression. Cas9-sgRNA (pCut) plasmids for integrating into 23 loci, across all 16 yeast chromosomes.
    Depositor
    Aindrila Mukhopadhyay
    Available Since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only

  16. pEC1.2/EC1.1_spCas9_GFP

    Plasmid
    #161933
    Purpose
    CRISPR plasmid with spCas9 expressed under egg cell promoter - GFP seed selection
    Depositor
    Christian Fankhauser
    Type
    Empty backbone
    Use
    CRISPR
    Available Since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    161933-plasmid-map-sequence-id-320699

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  17. pEC1.2/EC1.1_spCas9_TagRFP

    Plasmid
    #161934
    Purpose
    CRISPR plasmid with spCas9 expressed under egg cell promoter - TagRFP seed selection
    Depositor
    Christian Fankhauser
    Type
    Empty backbone
    Use
    CRISPR
    Available Since
    Jan. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    161934-plasmid-map-sequence-id-320739

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  18. pFGC-pcoCas9

    Plasmid
    #52256
    Purpose
    A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant expression of Cas9 and multiple cloning sites for insertion of guide RNA
    Depositor
    Jen Sheen
    Insert
    35SPPDK:pcoCas9:NOS cassette
    Use
    CRISPR; Plant expression
    Promoter
    constitutive 35SPPDK promoter
    Available Since
    March 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    52256-plasmid-map-sequence-id-116013

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  20. pYJ28-AAVS1-XLone-dCas9-p300core

    Plasmid
    #131071
    Purpose
    Donor plasmid of targeted inducible-dCAS9-P300core knockin
    Depositor
    Xiaojun Lian
    Insert
    dCas9-p300core
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    131071-plasmid-map-sequence-id-270718

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Showing: 321 - 340 of 9771 results