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Plasmid#169392PurposeCell-free/mammalian expression vector for SARS-COV-2 virus ORF6 with N-term eGFPDepositorInsertSARS-COV-2 _ORF6 (ORF6 SARS-Cov2)
UseCell-free expression vectorTagsHis and eGFPExpressionMutationPromoterT7- CMV/CAGAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
563aa ELL2 pEF4B
Plasmid#127273PurposeExpresses 563aa human WT ELL2DepositorInsertELL2 truncated at SphI (ELL2 Human)
UseTagsExpressionMammalianMutationContains 564 N-terminal amino acids ELL2, termina…PromoterpEF1aAvailable sinceNov. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
Human/mouse ELL2
Plasmid#127254PurposeExpresses a human/mouse chimera that increases ELL2 expression in mammalian cellsDepositorUseOtherTagsHis6XExpressionMammalianMutationN terminal amino acids (1-287) are human, C termi…PromoterpEF1aAvailable sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
ELL3 Streptag CtoA
Plasmid#127283PurposeExpresses mouse ELL3 with streptactin tag; To interrogate if the mutation decreases putative Myc activityDepositorInsertELL3 (NM_145973) (ELL3 Mouse)
UseTagsTwin strep-tagExpressionMammalianMutationCEYLHNK to AEYLHQKPromoterEF1aAvailable sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA tagged Sph ELL2
Plasmid#127270PurposeFor in vitro translation of human WT ELL2 with internal HA tagDepositorInsertELL2 (ELL2 Human)
UseIn vitro translationTagsExpressionMutationWT Mets and HA tag at SphI site (8th Met)PromoterT7Available sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA tagged mid ELL2
Plasmid#127274PurposeExpresses human ELL2 with HA tag in the middle that does not disrupt functionDepositorInsertELL2 with HA at XbaI (ELL2 Human)
UseTagsExpressionMammalianMutationHA inserted at XbaI after Met 186PromoterpEF1aAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met138ILeu, Met186ILeu ELL2
Plasmid#127265PurposeFor in vitro translation of human ELL2 with Met138ILeu, Met186ILeuDepositorInsertELL2 (Met138ILeu, Met186ILeu) (ELL2 Human)
UseIn vitro translationTagsExpressionMutationTwo Mets (138,186) to ILeu QuikchangePromoterT7Available sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met133ILeu, Met186ILeu ELL2
Plasmid#127264PurposeFor in vitro translation of human ELL2 with Met133ILeu, Met186ILeuDepositorInsertELL2 (Met133ILeu, Met186ILeu) (ELL2 Human)
UseIn vitro translationTagsExpressionMutationTwo Mets (133,186 )to ILeu QuikchangePromoterT7Available sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA tagged Xba ELL2
Plasmid#127268PurposeFor in vitro translation of WT ELL2 with HA tag inserted near N-termDepositorInsertELL2 (ELL2 Human)
UseIn vitro translationTagsExpressionMutationELL2mRNA+polyA with HA tag inserted at aa12 betwe…PromoterT7Available sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
564aa ELL2 bluescript
Plasmid#127267PurposeFor in vitro translation of human ELL2 lacking COOH regionDepositorInsertELL2 (ELL2 Human)
UseIn vitro translationTagsExpressionMutationAddgene Plasmid 127256--SphI site filled in, then…PromoterT7Available sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PTEN-L247_P248delLP
Plasmid#116624PurposeLentiviral expression of PTEN L247_P248delLPDepositorInsertPTEN (PTEN Human)
UseLentiviralTagsExpressionMutationL247_P248delLPPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PIK3R1-L372_R373delLR
Plasmid#116598PurposeLentiviral expression of PIK3R1 L372_R373delLRDepositorInsertPIK3R1 (PIK3R1 Human)
UseLentiviralTagsExpressionMutationL372_R373delLRPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PIK3R1-L380delL
Plasmid#116599PurposeLentiviral expression of PIK3R1 L380delLDepositorInsertPIK3R1 (PIK3R1 Human)
UseLentiviralTagsExpressionMutationL380delLPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-SpELL pBacPAK8
Plasmid#15317DepositorInserteleven nineteen lysine rich in leukemia (ell1 Fission Yeast)
UseBaculovirus constructionTagsFLAGExpressionMutationPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pmCell-Free_KA2_ZIKV NS2B
Plasmid#145364PurposeCell-free/mammalian expression vector for Zika virus NS2B protein with N-term mCherryDepositorInsertZIKV NS2B
UseTags8xHis and mCherryExpressionBacterialMutationPromoterT7- CMV/CAGAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAAV-mDlx-GFP-Fishell-1
Plasmid#83900PurposeGFP expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer elementDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InserteGFP
UseAAVTagsExpressionMutationPromotermDlxAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
Yellow Cameleon-Nano15/pBig
Plasmid#51962PurposeExpression of Yellow Cameleon-Nano15, an ultrasensitive Ca2+ indicator with Kd = 15 nM, in DictyosteliumDepositorInsertYellow Cameleon-Nano15
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
Yellow Cameleon-Nano65/pcDNA3
Plasmid#51965Purposemammalian expression of Yellow Cameleon-Nano65, an ultrasensitive Ca2+ indicator with Kd = 65 nMDepositorInsertYellow Cameleon-Nano65
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-mDlx-GCaMP6f-Fishell-2
Plasmid#83899PurposeGCaMP6f expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer elementDepositorHas ServiceAAV Retrograde, AAV1, and AAV9InsertGCaMP6f
UseAAVTagsExpressionMammalianMutationPromotermDlxAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
Notch3 intracellular domain-pcw107-V5
Plasmid#64623Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertNOTCH3 (NOTCH3 Human)
UseLentiviralTagsV5ExpressionMutationintracellular domain *PromoterPGKAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only