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pFastBac-rAPOBEC1-pAG-hADAR2dd

Plasmid

#248100

Purpose

Expresses rAPOBEC1-pAG-hADAR2dd in insect cells

Depositor

Yangming Wang

Article

Cheng et al Nat Methods. 2025 Sep;22(9):1824-1835. d

Inserts

rAPOBEC1 (Apobec1 Rat)
protein A/G
hADAR2dd (ADARB1 Human)

Tags

His and Strep

Expression

Insect

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pFastbac-Dual-StrepII-BARD1

Plasmid

#137166

Purpose

Expresses human StrepII-tagged BARD1 for purification from baculovirus/insect cell system

Depositor

Andrew Deans

Article

Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

Insert

BARD1 (BARD1 Human)

Tags

StrepII

Expression

Insect

Mutation

fully synthetic, codon optimized for insect expre…

Promoter

p10

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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EB3N-VVDfast-mCl3-iLID

Plasmid

#190165

Purpose

Expresses EB3N-VVDfast-mCl3-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.

Depositor

Anna Akhmanova

Article

Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

Insert

EB3N-VVDfast-mClover3-iLID

Tags

-VVDfast-mClover3-iLID

Expression

Mammalian

Mutation

1-189 aa from EB3

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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FASTHDR-Cterm-mRuby3-Zeo

Plasmid

#167206

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mRuby3 and selection with Zeocin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

mRuby3

Expression

Mammalian

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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EB3N-VVDfast-Halo-iLID

Plasmid

#190166

Purpose

Expresses EB3N-VVDfast-Halo-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.

Depositor

Anna Akhmanova

Article

Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

Insert

EB3N-VVDfast-Halo-iLID

Tags

-VVDfast-mClover3-iLID

Expression

Mammalian

Mutation

1-189 aa from EB3

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGEN-FastFUCCI (JDW 1494)

Plasmid

#242592

Purpose

A CAGGS driven expression vector containing FastFUCCI to label cell cycle state.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)

Expression

Mammalian

Promoter

CAGGS

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac1-Clover4-LUMABS-S1 RBD

Plasmid

#183491

Purpose

To express the Clover4-LUMABS-S1 RBD sensor for SARS-CoV-2 antibody dection.

Depositor

Jun Chu, Zongwen Jin

Article

Deng et al ACS Nano. 2021 Nov 2. doi: 10.1021/acsna

Insert

Clover4-LUMABS-S1 RBD

Expression

Insect

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac-rAPOBEC1-hADAR2dd-pAG

Plasmid

#248101

Purpose

Expresses rAPOBEC1-hADAR2dd-pAG in insect cells

Depositor

Yangming Wang

Article

Cheng et al Nat Methods. 2025 Sep;22(9):1824-1835. d

Inserts

rAPOBEC1 (Apobec1 Rat)
hADAR2dd (ADARB1 Human)
protein A/G

Tags

His and Strep

Expression

Insect

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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FASTHDR-tRNApromo-sgRNA-espCas91_1

Plasmid

#167210

Purpose

Plasmid for easy cloning of a sgRNA sequence. The Plasmid contain a tRNA promoter to facilitate the expression of any sgRNA sequence and also expresses eSpCas9(1.1)

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac HTB MBP-SARM1

Plasmid

#195763

Purpose

Purification of SARM1 from insect cells

Depositor

Hao Wu

Insert

SARM1 (SARM1 Human)

Tags

HIS-MBP-TEV

Expression

Insect

Promoter

polyhedrin

Available Since

Feb. 16, 2023

Availability

Academic Institutions and Nonprofits only

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FASTHDR-Cterm-HaloTag-Puromycin

Plasmid

#167208

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with HaloTag and selection with Puromycin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

HaloTag

Expression

Mammalian

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac Dual wt p110α/p85α

Plasmid

#199302

Purpose

co-expression of His-tagged p110α wt and Avi-tagged p85α wt proteins

Depositor

Peter Vogt

Article

Favalli et al Nat Chem. 2021 Jul;13(7):714. doi: 10.10

Insert

His-tagged p110α wt and Avi-tagged p85α wt (PIK3CA, PIK3R1 Human)

Tags

Avi and His

Expression

Insect

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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FASTHDR-Cterm-NanoLuc-Puromycin

Plasmid

#167209

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with NanoLuc and selection with Puromycin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

NanoLuc

Expression

Mammalian

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac_Dual_GST-TEV-EGFP-TBK1

Plasmid

#187830

Purpose

Plasmid for the expression and purification of GST-TEV-EGFP-TBK1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1631

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

TBK1 (TBK1 Human)

Tags

GST and TEV-mEGFP-GG Linker

Expression

Bacterial and Insect

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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FASTHDR-Cterm-mClover3-Puromycin

Plasmid

#167205

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mClover3 and selection with Puromycin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

mClover3

Expression

Mammalian

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CBA-DO(FAS)-GCaMP6s

Plasmid

#110135

Purpose

Cre-off expression of GCaMP6s

Depositor

Bernardo Sabatini

Article

Markowitz et al Cell. 2018 May 10. pii: S0092-8674(18)30

Insert

GCaMP6s

Use

AAV and Cre/Lox

Expression

Mammalian

Available Since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac Dual α1B/β3 Tubulin

Plasmid

#170552

Purpose

Encodes codon-optimized human α1B and β3 tubulin for expression in and purification from insect cells.

Depositor

Tarun Kapoor

Article

Ti et al Dev Cell. 2018 Oct 22;47(2):175-190.e5.

Inserts

TUBA1B (TUBA1B Human)
TUBB3 (TUBB3 Human)

Tags

AP linker, GS linker (GGSGG), His10 tag, L21 enha…

Expression

Insect

Available Since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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pFAST-BAC-Cdc20wt (2193)

Plasmid

#39850

Depositor

Jonathon Pines

Article

Nilsson et al Nat Cell Biol. 2008 Dec . 10(12):1411-20

Insert

Cdc20 (CDC20 Human)

Use

Baculovirus expression

Tags

His

Available Since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only
pFastBac-ATG101-ATG13 HORMA

Plasmid

#99332

Purpose

Mammalian expression of one subcomplex from the Human ULK1 complex

Depositor

James Hurley

Article

Qi et al Structure. 2015 Oct 6;23(10):1848-57. do

Inserts

ATG101 (ATG101 Human)
ATG13 (ATG13 Human)

Tags

GST

Expression

Insect

Promoter

p10 and pH

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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CMV-Fast-1 wild-type

Plasmid

#16521

Depositor

Bert Vogelstein

Article

Zhou et al Mol Cell. 1998 Jul . 2(1):121-7.

Insert

FAST-1 (FOXH1 Human)

Expression

Mammalian

Available Since

March 21, 2008

Availability

Academic Institutions and Nonprofits only