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Showing: 321 - 340 of 611 results

  3. pVH616

    Plasmid
    #169605
    Purpose
    Tier-2 vector encoding PmPGK1-driven Fluc and PCREm4-driven Nluc expression (PmPGK1-Fluc-pA::A2-pA::PCREm4-Nluc-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PCREm4 / PPGK
    Available since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    169605-plasmid-map-sequence-id-366619

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  4. pVH614

    Plasmid
    #169603
    Purpose
    Tier-2 vector encoding PmPGK1-driven Fluc and PCREm4-driven Nluc expression (PmPGK1-Fluc-pA::PCREm4-Nluc-pA::A3-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PCREm4 / PPGK
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169603-plasmid-map-sequence-id-365599

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  6. Antares-SEPLuc C1A4E

    Plasmid
    #141088
    Purpose
    precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES and weaker precursor of Nanoluc
    Depositor
    Jeak Ling Ding
    Article
    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41
    Insert
    Antares-T2A-puromycin-IRESv24-SEP-C1A4E
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Please see depositor comments
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    141088-plasmid-map-sequence-id-296867

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  9. pTS2303-Tier1-CoTC-PPGK-NLuc_TB

    Plasmid
    #169548
    Purpose
    Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    CoTC-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PPGK
    Available since
    June 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    169548-plasmid-map-sequence-id-364524

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  10. L2092-GPA-FullBRET-Retrovirus-TD142

    Plasmid
    #222877
    Purpose
    Retroviral vector encoding positive control receptor chain for BRET signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase, RH linker, CyOFP1.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA-NanoLuc-CyOFP1
    Use
    Retroviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    MSCV LTR
    Available since
    Aug. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    222877-plasmid-map-sequence-id-441620

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  12. pTS2307-Tier1-2xcHS4-PPGK-NLuc_TB

    Plasmid
    #169550
    Purpose
    Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a tandem repeat of a 5' cHS4 insulator sequence (2xcHS4-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    cHS4-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PPGK
    Available since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    169550-plasmid-map-sequence-id-366597

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  13. pTS2304-Tier1-Tactb-PPGK-NLuc_TB

    Plasmid
    #169549
    Purpose
    Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' Tactb insulator sequence (Tactb-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    Tactb-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PPGK
    Available since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    169549-plasmid-map-sequence-id-364990

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  14. pTS2308-Tier1-pGL3-PPGK-NLuc_TB

    Plasmid
    #169551
    Purpose
    Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    pGL3-insulator-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PPGK
    Available since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    169551-plasmid-map-sequence-id-364981

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  15. pAAV-anti-PDL1(KN035) PAGER(TF)

    Plasmid
    #230003
    Purpose
    Expresses anti-PDL1 PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)
    Depositor
    Alice Ting
    Article
    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-
    Insert
    IL2SP-Aro6-KN035-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    V360A/R361A on KORD
    Promoter
    CMV
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230003-plasmid-map-sequence-id-457206

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  16. pAAV-anti-GFP(LaG17) PAGER(TF)

    Plasmid
    #229997
    Purpose
    Expresses anti-GFP PAGER(TF) (high reversibility) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)
    Depositor
    Alice Ting
    Article
    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-
    Insert
    IL2SP-Aro6-LaG17-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    V360A/R361A on KORD
    Promoter
    CMV
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    229997-plasmid-map-sequence-id-457203

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  17. pAAV-anti-GFP(LaG2) PAGER(TF)

    Plasmid
    #229996
    Purpose
    Expresses anti-GFP PAGER(TF) (high sensitivity) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)
    Depositor
    Alice Ting
    Article
    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-
    Insert
    IL2SP-Aro6-LaG2-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    V360A/R361A on KORD
    Promoter
    CMV
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    229996-plasmid-map-sequence-id-457194

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  18. pAAV-anti-VEGF(Nb35) PAGER(TF)

    Plasmid
    #230000
    Purpose
    Expresses anti-VEGF PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)
    Depositor
    Alice Ting
    Article
    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-
    Insert
    IL2SP-Aro6-Nb35-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    V360A/R361A on KORD
    Promoter
    CMV
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230000-plasmid-map-sequence-id-457212

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  19. pAAV-anti-TNFalpha(Ozoralizumab) PAGER(TF)

    Plasmid
    #230001
    Purpose
    Expresses anti-TNFalpha PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)
    Depositor
    Alice Ting
    Article
    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-
    Insert
    IL2SP-Aro6-Ozoralizumab-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    V360A/R361A on KORD
    Promoter
    CMV
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230001-plasmid-map-sequence-id-457205

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  20. pAAV-anti-CCL2(8E10) PAGER(TF)

    Plasmid
    #230002
    Purpose
    Expresses anti-CCL2 PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)
    Depositor
    Alice Ting
    Article
    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-
    Insert
    IL2SP-Aro6-8E10-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    V360A/R361A on KORD
    Promoter
    CMV
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230002-plasmid-map-sequence-id-457201

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Showing: 321 - 340 of 611 results