We narrowed to 3,851 results for: sp6

1. pCRII-TOPO-dgat1b in situ probe

   Plasmid

   #232138

   Purpose

   zebrafish dgat1b anti-sense in situ probe, linearize with NotI, SP6 polymerase

   Depositor

   Steven Farber

   Article

   Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

   Insert

   dgat1b
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   SP6/T7

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pOO2-GW

   Plasmid

   #135097

   Purpose

   Gateway vector for expression of proteins in Xenopus oocytes

   Depositor

   Wolf Frommer

   Article

   Chen et al Nature. 2010 Nov 25;468(7323):527-32. do

   Type

   Empty backbone

   Use

   Bacterial expression vector for generating sp6 tr…

   Tags

   Expression

   Mutation

   Promoter

   SP6

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCRII-TOPO-dgat1a in situ probe

   Plasmid

   #232137

   Purpose

   zebrafish dgat1a anti-sense in situ probe, linearize with NotI, SP6 polymerase

   Depositor

   Steven Farber

   Article

   Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

   Insert

   dgat1a (dgat1a Zebrafish)
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   SP6/T7

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCRII-TOPO-mogat2 in situ probe

   Plasmid

   #232139

   Purpose

   zebrafish mogat2 anti-sense in situ probe, linearize with NotI, SP6 polymerase

   Depositor

   Steven Farber

   Article

   Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

   Insert

   mogat2 (mogat2 Zebrafish)
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   SP6/T7

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTLNX

   Plasmid

   #49032

   Purpose

   FX cloning Xenopus oocyte expression vector with SP6 promoter

   Depositor

   Eric Geertsma, Raimund Dutzler

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Use

   Xenopus oocytes expression

   Tags

   Expression

   Mutation

   Promoter

   SP6

   Available since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2+hSpCas9

   Plasmid

   #51815

   Purpose

   Encode hSpCas9 for in vitro transcription with Sp6

   Depositor

   Masato Kinoshita

   Article

   Ansai et al Biol Open. 2014 Apr 11;3(5):362-71. doi:

   Insert

   humanized S.pyogenes Cas9
   

   Use

   CRISPR; Medaka, zebrafish

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   SP6

   Available since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTLNXA7

   Plasmid

   #49033

   Purpose

   FX cloning Xenopus oocyte expression vector with SP6 promoter and N-terminal chicken alpha7 signal sequence

   Depositor

   Eric Geertsma, Raimund Dutzler

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Use

   Xenopus oocytes expression

   Tags

   chicken alpha7 signal sequence

   Expression

   Mutation

   Promoter

   SP6

   Available since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCSF107mT-GATEWAY-3'-FLAG

   Plasmid

   #67619

   Purpose

   A pCS2 based vector to generate a 3'-Flag tag using GATEWAY cloning technology

   Depositor

   Todd Stukenberg

   Article

   Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

   Type

   Empty backbone

   Use

   In vitro transcription or translation off of an s…

   Tags

   C-terminal Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and SP6

   Available since

   Aug. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCSF107mT-GATEWAY-3'-Myc tag

   Plasmid

   #67617

   Purpose

   A pCS2 based vector to generate a 3'-Myc tag using GATEWAY cloning technology

   Depositor

   Todd Stukenberg

   Article

   Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

   Type

   Empty backbone

   Use

   In vitro transcription or translation off of an s…

   Tags

   C-terminal Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and SP6

   Available since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCSF107mT-GATEWAY-3'-3HA

    Plasmid

    #67616

    Purpose

    A pCS2 based vector to generate a 3'-3HA tag using GATEWAY cloning technology

    Depositor

    Todd Stukenberg

    Article

    Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

    Type

    Empty backbone

    Use

    In vitro transcription or translation off of an s…

    Tags

    three inframe 3' HA tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and SP6

    Available since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pOLENTE

    Plasmid

    #80683

    Purpose

    DEST vector for cell-free in vitro expression

    Depositor

    Nico Dissmeyer

    Type

    Empty backbone

    Use

    Synthetic Biology; In vitro transcription and tra…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7 and SP6

    Available since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pmtb-t7-alpha-bungarotoxin

    Plasmid

    #69542

    Purpose

    For in vitro transcription of alpha-bungarotoxin mRNA that can be injected into embryos.

    Depositor

    Sean Megason

    Article

    Swinburne et al PLoS One. 2015 Aug 5;10(8):e0134005. doi

    Insert

    alpha-bungarotoxin
    

    Use

    Tol2, beta-actin promoter, sp6,t7 for zebrafish

    Tags

    Expression

    Mutation

    Promoter

    beta-actin, sp6, t7

    Available since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCSF107mT-GATEWAY-3'-LAP tag

    Plasmid

    #67618

    Purpose

    A pCS2 based vector to generate a 3'-LAP (eGFP-TEV-S) tag using GATEWAY cloning technology

    Depositor

    Todd Stukenberg

    Article

    Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

    Type

    Empty backbone

    Use

    In vitro transcription or translation off of an s…

    Tags

    C-termnal LAP (eGFP-TEV-S)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and SP6

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pCS2+ H2B-mCherry

    Plasmid

    #99265

    Purpose

    Contains a fusion of human histone H2B to fluorescent protein mCherry in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.

    Depositor

    Philipp Keller

    Article

    Wan et al Cell. 2019 Oct 3;179(2):355-372.e23. doi

    Insert

    mCherry
    

    Use

    Tags

    H2B

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. H2B CFP

    Plasmid

    #198059

    Purpose

    mCerulean fused to human H2B protein

    Depositor

    Amro Hamdoun

    Insert

    H2B
    

    Use

    Synthetic Biology; Sea urchin, mammal, zebrafish

    Tags

    mCerulean

    Expression

    Mutation

    Promoter

    sp6

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AbVec_IGHC

    Plasmid

    #183701

    Purpose

    Encode human IgG1 heavy chain constant region. For cloning variable region and mAb expression.

    Depositor

    David Nemazee

    Insert

    IGHG1 (IGHG1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AbVec_IGKC

    Plasmid

    #183702

    Purpose

    Encode human Ig kappa chain constant region. For cloning variable region and mAb expression.

    Depositor

    David Nemazee

    Insert

    IGKC (IGKC )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TFORF3027

    Plasmid

    #144503

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SP6 (SP6 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. AbVec_IGLC

    Plasmid

    #183703

    Purpose

    Encode human Ig lambda chain constant region. For cloning variable region and mAb expression.

    Depositor

    David Nemazee

    Insert

    IGLC2 (IGLC2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOO2-GW

    Plasmid

    #135097

    Purpose

    Gateway vector for expression of proteins in Xenopus oocytes

    Depositor

    Wolf Frommer

    Article

    Chen et al Nature. 2010 Nov 25;468(7323):527-32. do

    Type

    Empty backbone

    Use

    Bacterial expression vector for generating sp6 tr…

    Tags

    Expression

    Mutation

    Promoter

    SP6

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge