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pYTK001_E2Aq(2nd)

Plasmid

#190911

Purpose

2A peptide E2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

E2A

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK001_O2Aq(2nd)

Plasmid

#190918

Purpose

2A peptide O2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

O2A

Use

Synthetic Biology

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK001_P2Aq(1st)

Plasmid

#190912

Purpose

2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use as the first 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

P2A

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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Dendra-SpVasa

Plasmid

#185583

Purpose

for photoconversion

Depositor

Mamiko Yajima

Article

Wavreil et al Dev Biol. 2021 Oct;478:13-24. doi: 10.10

Insert

Vssa

Use

In vitro transcription (mrna synthesis)

Tags

Dendra

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pEPYCα1KN0002

Plasmid

#154612

Purpose

GoldenBraid level α effector

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_108 effector [MinSyn_108::CPMV::GAL4_AD::ɸC31_BD::ocsT]

Use

Synthetic Biology

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPYCα1KN0007

Plasmid

#154614

Purpose

GoldenBraid level α effector

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_108 effector [MinSyn_108::TMV::AvrXa27::ocsT]

Use

Synthetic Biology

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPYCα2rKN0009

Plasmid

#154616

Purpose

GoldenBraid level α luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_066 Nanoluc expression cassette [MinSyn_066::LucN::Flag::nosT]

Use

Synthetic Biology

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPYCα2rKN0010

Plasmid

#154617

Purpose

GoldenBraid level α luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_064 Nanoluc expression cassette [MinSyn_064::LucN::Flag::nosT]

Use

Synthetic Biology

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPKKα2KN0091

Plasmid

#154618

Purpose

GoldenBraid level α luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_064 Nanoluc expression cassette [MinSyn_064::LucN::Flag::g7T]

Use

Synthetic Biology

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPKKα2KN0092

Plasmid

#154619

Purpose

GoldenBraid level α luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_065 Nanoluc expression cassette [MinSyn_065::LucN::Flag::g7T]

Use

Synthetic Biology

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPKKα2KN0093

Plasmid

#154620

Purpose

GoldenBraid level α luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_066 Nanoluc expression cassette [MinSyn_066::LucN::Flag::g7T]

Use

Synthetic Biology

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPKKα2KN0100

Plasmid

#154621

Purpose

GoldenBraid level α luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_067 Nanoluc expression cassette [MinSyn_067::LucN::Flag::g7T]

Use

Synthetic Biology

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPKKα2KN0101

Plasmid

#154622

Purpose

GoldenBraid level α luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_068 Nanoluc expression cassette [MinSyn_068::LucN::Flag::g7T]

Use

Synthetic Biology

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPKKα2KN0102

Plasmid

#154623

Purpose

GoldenBraid level α luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_069 Nanoluc expression cassette [MinSyn_069::LucN::Flag::g7T]

Use

Synthetic Biology

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPYC1CB0305

Plasmid

#154625

Purpose

MoClo Level 1 reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_285 reporters [MinSyn_285::CPMV::HiBit::GUS::YFP::ocsT]

Use

Synthetic Biology

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPYC1CB0503

Plasmid

#154628

Purpose

MoClo Level 1 reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_000 reporters [MinSyn_000::CPMV::HiBit::GUS::YFP::ocsT]

Use

Synthetic Biology

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPYC1CB0477

Plasmid

#154626

Purpose

MoClo Level 1 reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_104 reporters [MinSyn_104::CPMV::HiBit::GUS::YFP::ocsT]

Use

Synthetic Biology

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPYC1CB0478

Plasmid

#154627

Purpose

MoClo Level 1 reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_108 reporters [MinSyn_108::CPMV::HiBit::GUS::YFP::ocsT]

Use

Synthetic Biology

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pSP64-mCherry

Plasmid

#135597

Purpose

Expression of mCherry mRNA for microinjection into S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

mCherry

Use

In vitro transcription (mrna synthesis)

Promoter

Unknown

Available Since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pTS38.3

Plasmid

#106266

Purpose

Polycistronic expression of ekivR, esyn1, luc using P2A

Depositor

Vera Meyer

Article

Schuetze et al Microb Cell Fact. 2017 Sep 25;16(1):162.

Insert

ekivR-P2A-esyn1-P2A-luc

Use

Luciferase and Synthetic Biology

Promoter

Tet-On

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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