We narrowed to 8,596 results for: dykddddk
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Plasmid#137827PurposeMESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal WT TEV protease, M cleavage sequence, and tTA transcription factorDepositorInsertFRB-CD28TMD-CTEVp(WT)-CS(M)-tTA
Tags3x FLAGExpressionMammalianPromoterCMVAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
FLAG-IntCCCre
Plasmid#160506PurposeExpresses IntCCCre, tagged with FLAG, in mammalian cells in split intern-mediated split-Cre recombinase applicationsDepositorInsertIntCCCre
UseCre/LoxTagsFLAGExpressionMammalianPromoterCMVAvailable SinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
GPX8 rescue PLAK1
Plasmid#161517PurposeRescues the expression of GPX8 in GPX8 plenti-CRISPR KO cellsDepositorAvailable SinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-FLAG-human NPM1-C1
Plasmid#160693PurposeMammalian expression of FLAG-tagged NPM1 C1 deletion mutant lacking nucleic acid-binding domain (NBD).DepositorAvailable SinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A mKate2-hu_ncOGT K842M
Plasmid#154284PurposeEntry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; K842M variant, catalytic dead)DepositorInsertO-GlcNAc Transferase (OGT Human)
UseGateway CloningTags2x FLAG and mKate2MutationK842M mutation (Catalytic Inactive)Available SinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV3TAG8li-hSTT3A-3FLAG
Plasmid#159140PurposeHu STT3A -flag mammalian expression vector.DepositorInsertSTT3 oligosaccharyltransferase complex catalytic subunit A (STT3A Human)
Tags3x FLAGExpressionMammalianPromoterCMVAvailable SinceNov. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-SARS2-S-FKO (NC-flag)
Plasmid#159363PurposeMammalian expression of SARS-CoV-2 S protein (FKO: furin-cleavage site mutant) with the flag tag at N- and C-termini.DepositorInsertSARS-CoV-2 S protein (S SARS-CoV-2)
TagsFlag and FlagExpressionMammalianMutationFurin-cleavage site mutationPromoterCMVAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS_FLAG-Mma PylRS
Plasmid#154762Purposeplasmid with Mma PylRS for amber suppression in transient or stable, piggybac-mediated, integrationDepositorAvailable SinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-SARS2-S-G614 (NC-flag)
Plasmid#156419PurposeMammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.DepositorInsertSARS-CoV-2 S protein (S SARS CoV2)
TagsFlagExpressionMammalianMutationD614GPromoterCMVAvailable SinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
RRL.sin.cPPT.CMV/Flag-E2-crimson.IRES-puro.WPRE (MT06)
Plasmid#139448PurposeLentiviral vector to ectopically express Flag-tagged E2-crimson from CMV promoterDepositorInsertFLAG-tag E2-crimson
UseLentiviralTagsFLAGExpressionMammalianPromoterCMVAvailable SinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgL-FLAG (TB40/E)
Plasmid#136449PurposeMammalian expression plasmid for HCMV glycoprotein L (TB40/E strain) with FLAG tagDepositorInsertglycoprotein L (UL115 Human betaherpesvirus 5)
TagsShort SGGSG linker and FLAG tagExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
MmSphk1
Plasmid#118599PurposeExpresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate in miceDepositorInsertMus musculus sphingosine kinase 1
TagsFLAGExpressionYeastPromoterGAL1, 10Available SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCX-SponGee-Kras
Plasmid#134777Purposemammalian expression of SponGee-Kras, a plasma membrane-targeted but lipid raft-excluded variant of the cGMP buffer SponGee that prevents downstream signalingDepositorInsertSponGee-Kras
TagsFLAG and mRFPExpressionMammalianPromoterCAGAvailable SinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pB1H2 UV5 Zif268-5ala (2-hybrid bait plasmid)
Plasmid#128165PurposeUV5 promoter drives expression of Zif268-5alanine peptide fusion. Pair with pGHUC w-ERBIN as a negative control (doesn't turn on HIS3/GFP).DepositorInsert10 amino acid glycine/serine linker + 5alanines
UseSynthetic BiologyTagsFLAG and zif268ExpressionBacterialPromoterUV5Available SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-Flag-PKMYT1_IDG-K
Plasmid#135277PurposeGateway destination clone of PKMYT1 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomicsDepositorInsertFlag-PKMYT1 (PKMYT1 Human)
UseLentiviral; Gateway destinationTagsFlagExpressionMammalianPromoterCMVAvailable SinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-RNF168 delta RING
Plasmid#133982Purposemammalian expression vector of Flag tagged RNF168 where the N-terminus of RNF168 has been deleted (including RING domain)DepositorAvailable SinceNov. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA6-C-3XFLAG-h-Lmbr1l
Plasmid#123462DepositorAvailable SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
CaMKIV-DCTK75E
Plasmid#126423PurposeA kinase-dead mutant of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)DepositorInsertcalcium/calmodulin-dependent protein kinase IV kinase-dead mutant (CAMK4 Human)
TagsFLAGExpressionMammalianMutationchanged lysine 75 to glutamic acidAvailable SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-M-Flag-BAP1-H169Q
Plasmid#125847Purposeexpress mutant BAP1-H169QDepositorAvailable SinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HA-Flag-natT1R2 FS1
Plasmid#113955Purposemammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variantDepositorInsertT1R2 (TAS1R2 Human)
TagsFLAG and Signal/leader sequence from influenza he…ExpressionMammalianMutationdelete N-terminal 21 residues, one base pair dele…PromotercmvAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only