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pCS2z-tal1

Plasmid

#164656

Purpose

for the synthesis of zebrafish tal1 mRNA

Depositor

Didier Stainier

Article

Mattonet et al Sci Adv. 2022 Sep 2;8(35):eabn2082. doi:

Insert

tal1 (tal1 Zebrafish)

Use

Mrna synthesis

Promoter

SP6

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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p3E-DrCav1

Plasmid

#194290

Purpose

Multisite gateway entry clone for expression of codon optimised zebrafish caveolin1 with fusion tag at the N-terminus. Parton lab clone KRT

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

caveolin1 (cav1.S Frog)

Use

Multisite gateway entry vector

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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375 pME-fli1a

Plasmid

#49001

Purpose

full length coding sequence for zebrafish fli1a gene in gateway middle entry vector

Depositor

Nathan Lawson

Article

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

friend leukemia integration 1a (fli1 Zebrafish)

Use

Gateway middle entry

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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EGFP-Bin1a

Plasmid

#176013

Purpose

Mammalian expression of zebrafish EGFP-Bin1a. Parton lab clone HYA

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Bin1a

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+ oDam_f_GFPoNLS

Plasmid

#85819

Purpose

iDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

Depositor

Joachim Wittbrodt

Article

Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

Insert

oDam_f_GFPoNLS

Tags

oNLS (optimized nuclear localization signal) C te…

Expression

Bacterial, Insect, Mammalia…

Mutation

L122A. Codon optimization compatible to work in M…

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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p3E-DysF

Plasmid

#109544

Purpose

Multisite gateway vector for 3' tagging with Dysferlin

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Dysferlin (dysf Zebrafish)

Use

Zebrafish plasmids

Mutation

Please see Depositor Comments

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-Clta

Plasmid

#109585

Purpose

Multisite gateway vector for 3' tagging with Clathrin light chain a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Clathrin light chain a (clta Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pdU6-2_sgRNA-d5-HT1AR

Plasmid

#221820

Purpose

Plasmid to express gRNA (gaccagtccactaccgcagc) for editing at the end of Drosophila 5-HT1AR coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

d5-HT1AR gRNA (5-HT1A Fly)

Use

CRISPR

Expression

Insect

Promoter

zebrafish U6 promoter (U6b)

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pdU6-2_sgRNA1-d5-HT1BR

Plasmid

#221821

Purpose

Plasmid to express gRNA1 (gaaaatttgatttcaactga) for editing at the end of Drosophila 5-HT1BR coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

d5-HT1BR gRNA1 (5-HT1B Fly)

Use

CRISPR

Expression

Insect

Promoter

zebrafish U6 promoter (U6b)

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pdU6-2_sgRNA2-5-HT2AR-isoformD

Plasmid

#221824

Purpose

Plasmid to express gRNA2 (ctgaagacataattacgtgg) for editing at the end of Drosophila 5-HT2AR isoform D coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

d5-HT2AR isoform D gRNA2 (5-HT2 Fly)

Use

CRISPR

Expression

Insect

Promoter

zebrafish U6 promoter (U6b)

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pdU6-2_sgRNA1-5-HT2AR-isoformBFH

Plasmid

#221825

Purpose

Plasmid to express gRNA1 (cgctatcggtctgtgacaga) for editing at the end of Drosophila 5-HT2AR isoforms B, F and H coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

d5-HT2AR isoforms BFH gRNA1 (5-HT2 Fly)

Use

CRISPR

Expression

Insect

Promoter

zebrafish U6 promoter (U6b)

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pdU6-2_sgRNA-d5-HT7R

Plasmid

#221829

Purpose

Plasmid to express gRNA (ggcgagggagagctttctct) for editing at the end of Drosophila 5-HT1AR coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

d5-HT7R gRNA (5-HT7 Fly)

Use

CRISPR

Expression

Insect

Promoter

zebrafish U6 promoter (U6b)

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG3-drAgo2DY-EF

Plasmid

#221733

Purpose

Expression of zebrafish Ago2 with D651E and Y680F mutations and N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2DY-EF (ago2 Zebrafish)

Tags

3xFLAG

Expression

Mammalian

Mutation

D651E and Y680F in active site

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-hsAgo2F-Y

Plasmid

#221722

Purpose

Expression of human Ago2 with F666Y mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2F-Y (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Mutation

F666Y in active site

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-hsAgo2E-D

Plasmid

#221721

Purpose

Expression of human Ago2 with E637D mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2E-D (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Mutation

E637D in active site

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-hsAgo2D-A

Plasmid

#221720

Purpose

Expression of human Ago2 with D669A mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2D-A (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Mutation

D669A in active site

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pTol2zmiRFP-EF1-2xCMV-EF1-mClover3

Plasmid

#196112

Purpose

mammalian/zebrafish expression of zmiRFP-EF1-2xCMV-EF1-mClover3

Depositor

Reinhard Köster

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

zmiRFP-EF1-2xCMV-EF1-mClover3

Expression

Mammalian

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2zmiRFP2-EF1-2xCMV-EF1-mClover3

Plasmid

#196111

Purpose

mammalian/zebrafish expression of zmiRFP2-EF1-2xCMV-EF1-mClover3

Depositor

Reinhard Köster

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

zmiRFP2-EF1-2xCMV-EF1-mClover3

Expression

Mammalian

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2zTagRFP658-EF1-2xCMV-EF1-mClover3

Plasmid

#196109

Purpose

mammalian/zebrafish expression of zTagRFP658-EF1-2xCMV-EF1-mClover3

Depositor

Reinhard Köster

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

zTagRFP658-EF1-2xCMV-EF1-mClover3

Expression

Mammalian

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2zemiRFP2-EF1-2xCMV-EF1-mClover3

Plasmid

#196110

Purpose

mammalian/zebrafish expression of zemiRFP2-EF1-2xCMV-EF1-mClover3

Depositor

Reinhard Köster

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

zemiRFP2-EF1-2xCMV-EF1-mClover3

Expression

Mammalian

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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