We narrowed to 3,553 results for: lenti crispr cas9 plasmids

1. pCAT009

   Plasmid

   #171635

   Purpose

   Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting B2M and CAG promoter expressing mTagBFP2

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-B2M
   mTagBFP2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAT007

   Plasmid

   #171634

   Purpose

   Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and CAG promoter expressing mTagBFP2

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-TRAC
   mTagBFP2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJRH056

   Plasmid

   #171637

   Purpose

   Plasmid containing a U6 promoter expressing a spyCas9 tdTom sgRNA298 and CAG promoter expressing mTagBFP2

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-tdTomato
   mTagBFP2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAT024

   Plasmid

   #171636

   Purpose

   Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting BFP and CAG promoter expressing mTagBFP2

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-BFP
   mTagBFP2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. SGL40C-H1.EFS.RFP657

   Plasmid

   #69148

   Purpose

   Advanced lentiviral Vector for sgRNA (H1 Promoter) delivery with RFP657 expression (EFS Promoter)

   Depositor

   Dirk Heckl

   Article

   Reimer et al Haematologica. 2017 Jun 1. pii: haematol

   Inserts

   sgRNA
   EFS
   tagRFP657
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. SGL40C.EFS.RFP657

   Plasmid

   #69147

   Purpose

   Advanced lentiviral Vector for sgRNA (hU6 Promoter) delivery with RFP657 expression (EFS Promoter)

   Depositor

   Dirk Heckl

   Article

   Reimer et al Haematologica. 2017 Jun 1. pii: haematol

   Inserts

   sgRNA
   EFS
   tagRFP657
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Immune checkpoint (library 2) Backbone

   Plasmid

   #192363

   Purpose

   Plasmid vector for in vivo immune checkpoint screening library (for checkpoint library)

   Depositor

   Lijia Ma

   Article

   Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. T cell activation (library 1) Backbone

   Plasmid

   #192364

   Purpose

   Plasmid vector for in vitro Jurkat activation screening (for immune library)

   Depositor

   Lijia Ma

   Article

   Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLKO.1-puro U6 sgRNA BfuAI stuffer

   Plasmid

   #50920

   Purpose

   U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites

   Depositor

   Rene Maehr, Scot Wolfe

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLex307-UCOE-SFFV-puroR-MCP-MLLx3-HSF1

    Plasmid

    #218558

    Purpose

    Encodes SFFV promoter-driven MCP-MLLx3-HSF1 with an N-terminal bipartite SV40 NLS, along with puromycin resistance

    Depositor

    Alejandro Chavez

    Insert

    MCP-MLLx3-HSF1
    

    Use

    Lentiviral

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    Available since

    Aug. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLex307-UCOE-SFFV-puroR-MCP-P65-HSF1

    Plasmid

    #218559

    Purpose

    Encodes SFFV promoter-driven MCP-P65-HSF1 with an N-terminal bipartite SV40 NLS, along with puromycin resistance

    Depositor

    Alejandro Chavez

    Insert

    MCP-P65-HSF1
    

    Use

    Lentiviral

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    Available since

    Aug. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLEX307_CR-PDHA2S291D

    Plasmid

    #115200

    Purpose

    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

    Depositor

    Mohan Babu

    Article

    Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

    Insert

    CR (CRISPR/Cas9-resistant)-PDHA2S291D (PDHA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    c.321C>T (silent when translated to protein, c…

    Promoter

    EF1alpha

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLEX307_CR-PDHA2S293D

    Plasmid

    #115201

    Purpose

    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S293D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

    Depositor

    Mohan Babu

    Article

    Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

    Insert

    CR (CRISPR/Cas9-resistant)-PDHA2S293D (PDHA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    c.321C>T (silent when translated to protein, c…

    Promoter

    EF1alpha

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLEX307_CR-PDHA2S291D/S293D

    Plasmid

    #115202

    Purpose

    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291D/S293D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

    Depositor

    Mohan Babu

    Article

    Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

    Insert

    CR (CRISPR/Cas9-resistant)-PDHA2S291D/S293D (PDHA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    c.321C>T (silent when translated to protein, c…

    Promoter

    EF1alpha

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCG-scr

    Plasmid

    #229847

    Purpose

    Expresses wild-type Cas9 and scrambled gRNA.

    Depositor

    Natsuko Chiba

    Insert

    guide RNA with scrambled sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLex307-UCOE-SFFV-puroR-MCP-HSF1-VIRF2

    Plasmid

    #218557

    Purpose

    Encodes SFFV promoter-driven MCP-HSF1-VIRF2 with an N-terminal bipartite SV40 NLS, along with puromycin resistance

    Depositor

    Alejandro Chavez

    Insert

    MCP-HSF1-VIRF2
    

    Use

    Lentiviral

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    Available since

    Aug. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSB700

    Plasmid

    #64046

    Purpose

    Lentiviral vector for expressing U6 sgRNA and CAGGS Cerulean fluorescent protein

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

    Type

    Empty backbone

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, CAGGS

    Available since

    May 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAT001

    Plasmid

    #171061

    Purpose

    Plasmid for expressing HIV-1 envelope glycoprotein

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Insert

    HIV-1 Env
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Nme2sgRNA_pLKO

    Plasmid

    #119926

    Purpose

    Nme2Cas9 U6-driven sgRNA cassette

    Depositor

    Erik Sontheimer

    Article

    Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

    Insert

    NmeCas9 sgRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCG-ACTB-C4

    Plasmid

    #229846

    Purpose

    Expresses wild-type Cas9 and gRNA for ACTB gene.

    Depositor

    Natsuko Chiba

    Insert

    guide RNA for ACTB gene
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge