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pET28-hisSumo-KE-Des27.4

Plasmid

#248000

Purpose

Expression of synthetic Kemp eliminase (Des27.4)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.4

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-hisSumo-KE-Des27.12

Plasmid

#248007

Purpose

Expression of synthetic Kemp eliminase (Des27.12)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.12

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des61.1

Plasmid

#248010

Purpose

Expression of synthetic Kemp eliminase (Des61.1)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des61.1

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.5

Plasmid

#248001

Purpose

Expression of synthetic Kemp eliminase (Des27.5)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.5

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des61

Plasmid

#248009

Purpose

Expression of synthetic Kemp eliminase (Des61)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des61

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-hisSumo-KE-Des27.2

Plasmid

#247998

Purpose

Expression of synthetic Kemp eliminase (Des27.2)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.2

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.6

Plasmid

#248002

Purpose

Expression of synthetic Kemp eliminase (Des27.6)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.6

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.11

Plasmid

#248006

Purpose

Expression of synthetic Kemp eliminase (Des27.11)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.11

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.7

Plasmid

#248003

Purpose

Expression of synthetic Kemp eliminase (Des27.7)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.7

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.9

Plasmid

#248004

Purpose

Expression of synthetic Kemp eliminase (Des27.9)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.9

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.13

Plasmid

#248008

Purpose

Expression of synthetic Kemp eliminase (Des27.13)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.13

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-gLuc-DR_pCMV-RedFireFly.gb

Plasmid

#242435

Purpose

Gaussia Luciferse with a Direct Repeat sequence in the 3’UTR recognised by the CasRx under the control of the CMV promoter, and a RedFirefly Luciferase driven by a CMV promoter.

Depositor

Diego Di Bernardo

Article

De Carluccio et al Nat Commun. 2024 Apr 17;15(1):3311. doi:

Insert

Gaussia Luciferase & Red Firefly Luciferase

Use

CRISPR and Luciferase

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-TO-CasRx(NLS)-T2A-mCherry

Plasmid

#242433

Purpose

CasRx (NLS-RfxCas13d-NLS) for RNA targeting under the CMV-TO promoter (CMV with two Tet-Operators)

Depositor

Diego Di Bernardo

Article

De Carluccio et al Nat Commun. 2024 Apr 17;15(1):3311. doi:

Insert

CasRx

Use

CRISPR

Tags

Tag / Fusion Protein: T2A mCherry

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET-hisTdT

Plasmid

#216674

Purpose

Expresses his-tagged N-terminus truncated TdT in E. coli hosts

Depositor

Po-Min Chiang

Article

Hsueh et al PLoS Biol. 2025 Dec 15;23(12):e3003537.

Insert

terminal deoxynucleotidyl transferase

Tags

his-tag

Expression

Bacterial

Mutation

1-138 truncation

Promoter

T7

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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HisH2A-InteinCBD-H2B

Plasmid

#240442

Purpose

Expresses H2A-Intein-Chitinbinding domain bound to H2B

Depositor

Mark Glover

Article

Panigrahi et al Nucleic Acids Res. 2025 Oct 14;53(19):gk

Inserts

HisH2A-Intein-CBD
H2B (H2B )

Tags

C-terminal fusion with intein-chitin binding doma…

Expression

Bacterial

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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p15GFPkv

Plasmid

#239039

Purpose

Expresses a + positively charged GFP variant (+15GFPKv) in mammalian cells

Depositor

Felipe Quiroz

Article

Avecilla et al ACS Sens. 2025 Mar 28;10(3):1857-1869. d

Insert

p15GFPKv-NES

Expression

Mammalian

Mutation

All surface-exposed Arg residues changed to Lysine

Promoter

CMV

Available Since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA-Donor_CBh

Plasmid

#241376

Purpose

Bxb1-GA donor plasmid for cloning of payloads <20 kb with CBh promoter for expressing landing pad enrichment marker

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.SynFLEX.(cyto).cpSFGFP.mIRFP670nano3

Plasmid

#244918

Purpose

cpSFGFP control with non-responsive mIRFP670nano3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

cpSFGFP.mIRFP670nano3

Use

AAV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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AGG1829 OpIE2-Bn

Plasmid

#242438

Purpose

Insect OpIE2 promoter expressing barnase

Depositor

Luke Alphey

Article

Nevard et al Commun Biol. 2025 Aug 4;8(1):1154. doi:

Insert

Barnase (J5X95_RS06160 Bacillus amyloliquefaciens)

Expression

Insect

Promoter

OpIE2

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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AGG1767 PUb-Bs-TRE-Bn

Plasmid

#242441

Purpose

Ae aegypti PUb promoter expressing barstar, and TRE expressing barnase

Depositor

Luke Alphey

Article

Nevard et al Commun Biol. 2025 Aug 4;8(1):1154. doi:

Inserts

Barstar
DsRED
barnase

Expression

Insect

Promoter

AePUb, Hr5/IE1, and TRE

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2197_pPV2-ZF10-NS3-p65

Plasmid

#239700

Purpose

BsaI Golden Gate assembly plasmid encoding the SynZiFTR ZF10-NS3-p65 CDS

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

ZF10-NS3-p65

Use

Synthetic Biology

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-U6-epegRNA eBAR3-SV40-puro

Plasmid

#240537

Purpose

Lentiviral eBAR3 backbone plasmid to insert epegRNAs (tevopreq1) of interest with puromycin resistance. The cloning site is BsmBI.

Depositor

Wensheng Wei

Article

Niu et al Nat Biotechnol. 2025 Jun 24. doi: 10.103

Insert

epegRNA-eBAR3

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-U6-epegRNA eBAR2-SV40-puro

Plasmid

#240536

Purpose

Lentiviral eBAR2 backbone plasmid to insert epegRNAs (tevopreq1) of interest with puromycin resistance. The cloning site is BsmBI.

Depositor

Wensheng Wei

Article

Niu et al Nat Biotechnol. 2025 Jun 24. doi: 10.103

Insert

epegRNA-eBAR2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Bxb1-GA-Bactin-mStayGold

Plasmid

#241380

Purpose

Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with B-actin promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mStayGold

Expression

Mammalian

Promoter

Bactin

Available Since

Sept. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Bxb1-GA-RSV-mStayGold

Plasmid

#241381

Purpose

Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with RSV promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mStayGold

Expression

Mammalian

Promoter

RSV

Available Since

Sept. 15, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GT-CBh-mStayGold

Plasmid

#241390

Purpose

Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with CBh promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mStayGold

Expression

Mammalian

Promoter

CBh

Available Since

Sept. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Bxb1-GT-Bactin-mStayGold

Plasmid

#241391

Purpose

Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with B-actin promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mStayGold

Expression

Mammalian

Promoter

Bactin

Available Since

Sept. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Bxb1-GT-PGK-mStayGold

Plasmid

#241392

Purpose

Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with PGK promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mStayGold

Expression

Mammalian

Promoter

PGK

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Bxb1-GT-CAG Donor

Plasmid

#241373

Purpose

Bxb1-GT donor plasmid with CAG promoter, WPRE and polyA signal for constitutive transgene expression (empty)

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Bxb1-GA-hEF1a-mStayGold

Plasmid

#241377

Purpose

Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with hEF1a promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mStayGold

Expression

Mammalian

Promoter

hEF1a

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Bxb1-GA-ShortCAG-mStayGold

Plasmid

#241385

Purpose

Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with ShortCAG promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mStayGold

Expression

Mammalian

Promoter

ShortCAG

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Bxb1-GA-PGK-mStayGold

Plasmid

#241384

Purpose

Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with PGK promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mStayGold

Expression

Mammalian

Promoter

PGK

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Bxb1-GA-Donor_UbC

Plasmid

#241375

Purpose

Bxb1-GA donor plasmid for cloning of payloads <20 kb with UbC promoter for expressing landing pad enrichment marker

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Bxb1-GA-SV40-mStayGold

Plasmid

#241382

Purpose

Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with SV40 promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mStayGold

Expression

Mammalian

Promoter

SV40

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
C-term_C-myc (4a)

Plasmid

#238907

Purpose

Plasmid insert contains the C-myc C-terminal tag sequence for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

C-term_C-myc

Use

Synthetic Biology

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET50del_SynPspA_H1-5

Plasmid

#223820

Purpose

Bacterial expression of SynPspA H1-5 with N-terminal 6xHis-Tag and 3C cleavage site

Depositor

Carsten Sachse

Article

Junglas et al Nat Struct Mol Biol. 2025 Jan;32(1):23-3

Insert

SynPspA

Tags

6xHis

Expression

Bacterial

Mutation

truncation of helix 0

Available Since

Sept. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET50del_SynPspA

Plasmid

#223818

Purpose

Bacterial expression of SynPspA with N-terminal 6xHis-Tag and 3C cleavage site

Depositor

Carsten Sachse

Article

Junglas et al Nat Struct Mol Biol. 2025 Jan;32(1):23-3

Insert

SynPspA

Tags

6xHis

Expression

Bacterial

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG3777_pGEEC584_pShip-EFS(promoter)-CMV(UTR)-mRuby2-bGH

Plasmid

#239746

Purpose

Plasmid expressing mRuby2 from the EFS canonical promoter with the CMV 5' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Tad-CBE(CCA2)

Plasmid

#235069

Purpose

Expresses Tad-CBE(CCA2) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(CCA2)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Tad-CBE(GCT1)

Plasmid

#235076

Purpose

Expresses Tad-CBE(GCT1) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(GCT1)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Tad-CBE(GCT2)

Plasmid

#235077

Purpose

Expresses Tad-CBE(GCT2) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(GCT2)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Tad-CBE(GCG1)

Plasmid

#235079

Purpose

Expresses Tad-CBE(GCG1) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(GCG1)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Tad-CBE(ACG1)

Plasmid

#235063

Purpose

Expresses Tad-CBE(ACG1) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(ACG1)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG3668_pGEEC578_pShip-EFS-rtTA-P2A-iRFP720-bGH

Plasmid

#239741

Purpose

Plasmid expressing a doxycycline-inducible transcriptional activator

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

rtTA-P2A-iRFP720

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG3831_pGEEC579_pShip-UbC(no_intron)-mRuby2-bGH

Plasmid

#239742

Purpose

Plasmid expressing mRuby from a UbC promoter lacking an intron, used for modRNA production

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG3774_pGEEC580_pShip-EF1a(no_intron)-mRuby2-bGH

Plasmid

#239743

Purpose

Plasmid expressing mRuby from an EF1a promoter lacking an intron, used for modRNA production

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG3775_pGEEC582_pShip-hPGK(promoter)-CMV(UTR)-mRuby2-bGH

Plasmid

#239744

Purpose

Plasmid expressing mRuby2 from the hPGK canonical promoter with the CMV 5' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG3776_pGEEC583_pShip-hPGK(promoter)-EFS(UTR)-mRuby2-bGH

Plasmid

#239745

Purpose

Plasmid expressing mRuby2 from the hPGK canonical promoter with the EFS 5' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG3778_pGEEC585_pShip-EFS(promoter)-hPGK(UTR)-mRuby2-bGH

Plasmid

#239747

Purpose

Plasmid expressing mRuby2 from the EFS canonical promoter with the hPGK 5' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG3779_pGEEC586_pShip-CMV(promoter)-EFS(UTR)-mRuby2-bGH

Plasmid

#239748

Purpose

Plasmid expressing mRuby2 from the CMV canonical promoter with the EFS 5' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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