We narrowed to 9,836 results for: pET

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pET28a-SnoopTag-AffiHER2-SpyTag

Plasmid

#216280

Purpose

Expresses an affibody to HER2 fused to SnoopTag and SpyTag, for bacterial expression

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

SnoopTag-AffiHER2-SpyTag

Use

Affinity Reagent/ Antibody

Tags

His6, SnoopTag, SpyTag, and Thrombin cleavage sit…

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET21b GFP-Rab10 Q68LHis

Plasmid

#186015

Purpose

GFP-Rab10 Q68LHis

Depositor

Suzanne Pfeffer

Article

Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

Insert

GFP-Rab10 Q68LHis (RAB10 Human)

Expression

Bacterial

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET15b+His SUMO PPM1H

Plasmid

#207333

Purpose

Bacterial expression of His SUMO PPM1H

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Tags

His-SUMO

Expression

Bacterial

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-YPet-WPRE

Plasmid

#193342

Purpose

AAV-mediated expression of YPet under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

YPet

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a_DmKHC[1-421]-SNAP-6xHis

Plasmid

#196975

Purpose

Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal SNAP tag and C-terminal 6xHis tag

Depositor

Lukas Kapitein

Article

Deguchi et al Science. 2023 Mar 10;379(6636):1010-1015

Insert

Kinesin Heavy Chain (Khc Fly)

Tags

6xHis and SNAP

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pET28-sfGFP[D134TAG+N150TAA]

Plasmid

#164582

Purpose

superfolder GFP with D134TAG and N150TAA mutations

Depositor

Ryan Mehl

Article

Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

Insert

sfGFP-134TAG+150TAA

Tags

6xHis

Expression

Bacterial

Mutation

D134TAG + N150TAA Mutations

Promoter

T7

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pETDuet-1_VNp-mNeongreen_CydAB-mCherry

Plasmid

#182391

Purpose

Bacterial expression of Vesicle Nucleating peptide-mNeongreen and cydAB-mCherry fusions

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Inserts

VNp-mNeongreen-His
cydAB-mCherry

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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Pet15b-Endosomal Escape Control

Plasmid

#186552

Purpose

Negative Control confirming the need for a CPP peptide in mediating internalization

Depositor

Jean Gariepy

Article

Mohammed et al J Control Release. 2012 Nov 28;164(1):58

Insert

6H-ETA-GFP

Tags

6xHis and EGFP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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Pet15b-Internalization and Endosomal Escape

Plasmid

#186551

Purpose

Internalization and Endosomal Escape to effectively route a protein cargo to the cytosol of cells

Depositor

Jean Gariepy

Article

Mohammed et al J Control Release. 2012 Nov 28;164(1):58

Insert

10R-8His-ETA-EGFP

Tags

8xHis and EGFP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-His6-Ubc9-K14R

Plasmid

#185759

Purpose

Bacterial expression of His6-Ubc9-K14R

Depositor

Jeffrey Bode

Article

Akimoto et al ACS Cent Sci. 2022 Feb 23;8(2):275-281.

Insert

SUMO-conjugating enzyme UBC9 (UBE2I Human)

Tags

Thrombin-cleavable His6-tag

Expression

Bacterial

Mutation

K14R

Promoter

T7

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2396-Tier1-PhCMV-4xCOX8_Ypet

Plasmid

#169534

Purpose

Tier-1 vector encoding PhCMV-driven yellow fluorescent protein YPet localized to the mitochondria (PhCMV-4xCOX8-YPet-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven miochondria-localized yellow fluorescent protein Ypet

Expression

Mammalian

Promoter

PhCMV

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-MBP-CBX1-eReader

Plasmid

#135089

Purpose

Bacterial expression of engineered CBX1 with enhanced binding to H3K9me

Depositor

Marcey Waters

Article

Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

Insert

CBX1 chromobox protein 1, engineered

Tags

6xhis-tag and MBP

Expression

Bacterial

Mutation

lysine 43 mutated to alanine; aspartic acid 59 mu…

Promoter

T7

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pETDuet-PcPT-ximD-ximE

Plasmid

#172654

Purpose

Production of decursinol in E. coli

Depositor

Minjuan Xu

Article

Bu et al ACS Synth Biol. 2020 Sep 18;9(9):2282-22

Insert

PcPT, XimD and XimE

Use

Synthetic Biology

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pET28-sfGFP-DogTag Loop B

Plasmid

#171780

Purpose

Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Asp102 and Asp103

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

sfGFP-DogTag Loop B

Tags

His6

Expression

Bacterial

Mutation

DogTag and linkers inserted between residues Asp1…

Promoter

T7

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pET28-sfGFP-SpyTag003 Loop A

Plasmid

#171776

Purpose

Expresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Val22 and Asn23

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

sfGFP-SpyTag003 Loop A

Tags

His6

Expression

Bacterial

Mutation

SpyTag003 and linkers inserted between residues V…

Promoter

T7

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pET11a_3xFlag-nsp10 (SARS-CoV-2)

Plasmid

#169157

Purpose

To express SARS-CoV-2 nsp10 in E. coli

Depositor

John Diffley

Article

Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

Insert

3xFlag-nsp5CS[VRLQ]-nsp10 (ORF1ab Synthetic, SARS-CoV-2)

Tags

3xFlag and nsp5CS[VRLQ]: predicted nsp5 protease …

Expression

Bacterial

Mutation

Codon optimised for E. coli

Promoter

T7

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-HaloTag7_dead_mt

Plasmid

#167267

Purpose

Overexpression of the dead variant (D106A) of HaloTag7 in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

HaloTag7

Tags

His x10

Expression

Bacterial

Mutation

D106A

Promoter

T7

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pET51b-Strep-hAGT-His

Plasmid

#167276

Purpose

Overexpression of the human o_-alkylguanine-dna alkyltransferase in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

hAGT (AGT Human)

Tags

His x10 and Strep-tag

Expression

Bacterial

Promoter

T7

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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WT N-His Ana GvpC in pET28a

Plasmid

#153294

Purpose

Expresses N-terminal His-tagged GvpC from Anabaena flos-aquae in E.coli

Depositor

Mikhail Shapiro

Article

Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

Insert

Gas vesicle protein C (N-terminal his-tagged)

Tags

His-tag

Expression

Bacterial

Mutation

Added a hexahistidine tag after the methionine at…

Promoter

T7

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pET28-His6-Ubc9-C93A

Plasmid

#133910

Purpose

Bacterial expression of His6-Ubc9-C93A

Depositor

Jeffrey Bode

Article

Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

Insert

SUMO-conjugating enzyme UBC9 (UBE2I Human)

Tags

Thrombin-cleavable His6-tag

Expression

Bacterial

Mutation

Cysteine 93 to Alanine

Promoter

T7

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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