We narrowed to 12,311 results for: pET

Showing: 3441 - 3460 of 12311 results

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pET28_mouse-pro-IL1B

Plasmid

#183386

Purpose

Overexpression of catalytic domain of inflammatory caspase in bacteria

Depositor

Jonathan Kagan

Publication

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

pro-IL1B (Il1b Mouse)

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pET28b-miniR-DBCO

Plasmid

#181984

Purpose

Used to label proteins with the MiniE genetically encoded tag

Depositor

Kimberly Beatty

Publication

Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

Insert

MiniR-DBCO

Tags

DBCO-Tag, His-Tag

Expression

Bacterial

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-E988K

Plasmid

#173945

Purpose

Bacterial expression of inactive PARP1 mutant (E988K attenuates catalytic and PAR-branching activity of PARP1)

Depositor

Joan Conaway

Publication

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-E988K (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

V762A, E988K

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-CATdeltaHD

Plasmid

#174811

Purpose

Bacterial expression of a hyperactive PARP1 CAT lacking the autoinhibitory HD subdomain

Depositor

Joan Conaway

Publication

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-CATdeltaHD (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Residues 678-787 replaced by eight-residue linker…

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaM-6

Plasmid

#172771

Purpose

Bacterial expression of anti-mCherry nanobody LaM-6, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-6

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-18

Plasmid

#172763

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-18; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Publication

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-18

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-11

Plasmid

#172762

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-11; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Publication

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-11

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-TEV-U1AF37MF77M

Plasmid

#168267

Purpose

Expresses human dmU1A with the F37M/F77M double mutant

Depositor

Joseph Wedekind

Publication

Srivastava et al Crystals (Basel). 2021 Mar;11(3). doi: 1

Insert

U1A RRM1 crystallization module (SNRPA Human)

Tags

6xHis tag N-terminus, TEV protease cleavage site

Expression

Bacterial

Mutation

changed F37M and F77M

Promoter

T7 promotor

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pET15-His-∆NGlpG

Plasmid

#155131

Purpose

Expresses N-terminally His-tagged E. coli GlpG (residues 87-276) from pET15b

Depositor

Sinisa Urban

Publication

Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

Insert

GlpG (glpG Escherichia coli)

Tags

His

Expression

Bacterial

Mutation

insert codes for GlpG residues 87-276

Promoter

T7

Available since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pET19b+H2B(T116C)

Plasmid

#157808

Purpose

Expression of human histone H2B with T116C mutation to facilitate maleimide chemistry

Depositor

Michael Rosen

Publication

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

H2B(T116C)

Expression

Bacterial

Mutation

T116C

Promoter

T7

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a His6Flag2AUF1(p45)

Plasmid

#135942

Purpose

Expresses AUF1p45 in E. coli

Depositor

Yimon Aye

Publication

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)

Tags

Flag2 and His6

Expression

Bacterial

Promoter

T7

Available since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-PKM2-R399E

Plasmid

#122691

Purpose

Express His-tagged PKM2 (R399E) protein in bacteria.

Depositor

Wen-Ching Wang

Publication

Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

Insert

Pyruvate kinase M2 isoform (PKM Human)

Tags

6His-tag

Expression

Bacterial

Mutation

R399E

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-PKM2-G415R

Plasmid

#122693

Purpose

Express His-tagged PKM2 (G415R) protein in bacteria.

Depositor

Wen-Ching Wang

Publication

Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

Insert

Pyruvate kinase M2 isoform (PKM Human)

Tags

6His-tag

Expression

Bacterial

Mutation

G415R

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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Brg1 Del4 pET28C

Plasmid

#122118

Purpose

Expression Brg1 amino acids 1446-1647 in bacteria

Depositor

Paul Herring

Publication

Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

Insert

Brg1 (SMARCA4 Human)

Tags

His-T7

Expression

Bacterial

Available since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pFS_0143-pET30-Fusicatenibacter_saccharivorans

Plasmid

#116952

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1, compatible with classic acquisition readout

Depositor

Randall Platt

Publication

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pET24a-VHH-2xTS

Plasmid

#109419

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to two tyrosine sulfation (TS) motifs. VHH-2xTS also contains a T7, HA, BAP and His6 epitope

Depositor

Martin Spiess

Publication

Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

Insert

anti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope

Tags

BAP, HA, His6, T7, and Tyrosine sulfation (TS) se…

Expression

Bacterial

Promoter

T7

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET28b-NmCas9-Flag

Plasmid

#109030

Purpose

Wild type Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

Depositor

Yan Zhang

Publication

Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

Insert

CRISPR-associated endonuclease Cas9

Tags

Flag Tag

Expression

Bacterial

Promoter

T7

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pET11a-dH3-dH4

Plasmid

#105502

Purpose

Co-expression of Drosophila histones H3 and H4

Depositor

James Kadonaga

Publication

Khuong et al J Biol Chem. 2017 Nov 24;292(47):19478-1

Insert

dH3-dH4

Available since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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pETM6-4F2-mCherry

Plasmid

#73421

Purpose

Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4F2.

Depositor

Mattheos Koffas

Publication

Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

Insert

Promoter 4F2 (orthogonal T7-lac variant)

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

P4F2 (orthogonal T7-lac variant)

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pET28-MRPP3-His

Plasmid

#67866

Purpose

bacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. His tag

Depositor

Walter Rossmanith

Publication

Vilardo et al Nucleic Acids Res. 2012 Dec;40(22):11583

Insert

MRPP3 (KIAA0391 Human)

Tags

His

Expression

Bacterial

Mutation

Amino acid 46 of recombinant MRPP3 is preceded by…

Promoter

T7

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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