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pX330-Flag-SpCas9-HF1 (without sgRNA)

Plasmid

#92102

Purpose

Expression plasmid for human codon-optimized high-fidelity SpCas9-HF1, px330-like backbone (without U6-sgRNA coding sequence)

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

3xFlag-NLS-Streptococcus pyogenes Cas9-HF1-NLS

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

N497A, R661A, Q695A, Q926A

Promoter

Cbh

Available Since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO hChR2(C128S/D156A)-mCherry

Plasmid

#35504

Purpose

AAV expression of Ef1a-driven, cre-dependent, stabilized step function opsin (SSFO) for optogenetic activation.

Depositor

Karl Deisseroth

Article

Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

Insert

hChR2(C128S/D156A)-mCherry

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

C128S and D156A

Promoter

Ef1a

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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CMV-VPR-S-L1-dCjCas9 MiniCAFE

Plasmid

#169910

Purpose

Expression of a truncated VPR-dCjCas9 fusion protein to activate gene expression.

Depositor

Zhili Rong

Article

Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

Insert

MiniCAFE

Use

CRISPR

Expression

Mammalian

Mutation

D8A, HNH-truncation (Δ495–609 aa) in CjCas9

Promoter

CMV

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pDestTol2-actinb-kcnj13-EGFP

Plasmid

#164940

Purpose

Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj13 fused with EGFP at the C terminus

Depositor

GuangJun Zhang

Article

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcnj13 (kcnj13 Zebrafish)

Use

Tol2 transposon destination vector

Tags

EGFP

Mutation

Stop codon deleted

Promoter

actinb (actb2) promoter

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-β-catenin_S23A,T40A,T41A,T112A

Plasmid

#190824

Purpose

For mammalian expression of β-catenin with 4 O-GlcNAc sites mutated (S23A, T40A, T41A and T112A) and GFP-tag on its N-terminus.

Depositor

Gerald Hart

Article

Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

Insert

β-catenin (CTNNB1 Chicken)

Tags

GFP

Expression

Mammalian

Mutation

S23A, T40A, T41A, T112A

Promoter

CMV

Available Since

Jan. 9, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pHH0103_DOCK5-1/1-IS1/2

Plasmid

#91303

Purpose

Protein expression and purification of human SH3 domain construct DOCK5-1/1-IS1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DOCK5-1/1-IS1/2 (DOCK5 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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prhaBAD-anti-mouse-Nb-Hia5

Plasmid

#239626

Purpose

Rhamnose inducible expression plasmid for anti-mouse nanobody fusion to Hia5 DNA adenine methyltransferase

Depositor

Aaron Straight

Article

Gamarra et al bioRxiv [Preprint]. 2025 Mar 14:2025.03.

Insert

Hia5

Tags

6HIS, MBP, TEV, and anti-Mouse Nanobody

Expression

Bacterial

Mutation

Codon optimized for bacterial expression

Promoter

rhaBAD

Available Since

July 7, 2025

Availability

Academic Institutions and Nonprofits only

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pKH3-TRIM28-C65A/C68A

Plasmid

#92199

Purpose

Mammalian overexpression vector for TRIM28; catalytically inactive (mutated on C65A and C68A)

Depositor

Huda Zoghbi

Article

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

Tif1b (TRIM28 Synthetic, Human)

Tags

HA

Expression

Mammalian

Mutation

Changed Cysteines 65 and 68 to Alanines.

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-Synapto-pHluorin

Plasmid

#168510

Purpose

Expression of Synapto-pHluorin for optical monitoring of presynaptic release in neurons.

Depositor

Franck Polleux

Article

Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

Insert

Synaptophysin-pHluorin (Syp Mouse)

Tags

pHluorin

Expression

Mammalian

Promoter

CAG

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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NA20 LN16

Plasmid

#101870

Purpose

Expresses HIV Env gene when transfected into 293-T cells for production of pseudovirus

Depositor

Paul Clapham

Article

Peters et al J Virol. 2004 Jul;78(13):6915-26.

Insert

Env (env )

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only
pLVX NPC1-FLAG-TiD

Plasmid

#199748

Purpose

Lentiviral construct to stably express NPC1 TurboID

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

NPC1 (NPC1 Human)

Tags

Flag and TurboID

Expression

Mammalian

Promoter

EF1a

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2-FUS-1-526aa-V5

Plasmid

#29609

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Tags

V5

Expression

Mammalian

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn FLEx-FRT Kir2.1-2A-GFP

Plasmid

#161577

Purpose

Expression of Kir2.1-2A-GFP in a Flp-dependent fashion

Depositor

Kevin Beier, Robert Malenka

Article

Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

Insert

Kir2.1-2A-GFP (Kcnj2 Mouse)

Use

AAV

Mutation

NA

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Kir2.1WT-2A-mScarlet-KASH

Plasmid

#203840

Purpose

Kir2.1 expression for silencing neurons and mScarlet-KASH expression

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Has Service

AAV8

Insert

Kir2.1 (Kcnj2 Mouse)

Use

AAV

Tags

2A-mScarlet-KASH

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pLenSyn2-EGFP-OMP25C

Plasmid

#71427

Purpose

Lentiviral expression of EGFP-OMP25C. For visualization of neuronal mitochondria.

Depositor

Henri Huttunen

Article

Kysenius et al PLoS One. 2014 Sep 5;9(9):e107129. doi:

Insert

OMP25 (172-‐206) (Synj2bp Rat)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 172-206 only

Promoter

synapsin

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-Syn-ChrimsonR-GFP

Plasmid

#58806

Purpose

AAV expression of ChrimsonR-GFP under the Syn promoter

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

K176R

Promoter

Syn

Available Since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pLEX307-SARS-CoV-2-3CL

Plasmid

#160278

Purpose

Allows for constituitive mammalian expression of the SARS-CoV-2 3CL protease via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pES001 MBP-GSK3β-HA-His

Plasmid

#154010

Purpose

Expresses MBP-GSK3β-HA-His (human Glycogen Synthase Kinase 3β as a fusion protein with MBP, HA, and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

GSK3B (GSK3B Human)

Tags

HA tag, His6, and MBP

Expression

Bacterial

Promoter

Tac

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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AAV hSYN DIO mGluR1-myc-FLAG

Plasmid

#166227

Purpose

AAV expressing cre-dependent mGluR1-myc-FLAG

Depositor

Jason Aoto

Article

Cai et al Neuron. 2021 Apr 7;109(7):1137-1149.e5.

Insert

metabotropic glutamate receptor 1 (Grm1 Mouse)

Use

AAV and Cre/Lox

Tags

Myc and FLAG

Expression

Mammalian

Promoter

human Synapsin

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_CDK4(R24C)-P2A-Hygro_Barcode

Plasmid

#170222

Purpose

Barcoded lentiviral vector to express CDK4 (R24C) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

CDK4 (CDK4 Human)

Use

Lentiviral

Mutation

R24C

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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