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Halo-BASU-His

Plasmid

#218451

Purpose

E coli expression vector for Halo-BASU-His biotin ligase

Depositor

Thorsten Stafforst

Article

Hanswillemenke et al Nat Chem Biol. 2024 Jan 17. doi: 10.1038

Insert

HaloTag-BASU-His fusion protein

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAS458-U6_CBA-mCherry

Plasmid

#195566

Purpose

Transient expression of mCherry; U6 with scaffold for sgRNA cloning in mammalian cells.

Depositor

Joanna Wysocka

Article

Spencley et al Mol Cell. 2023 May 18;83(10):1623-1639.e

Insert

mCherry

Expression

Mammalian

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pETM6-T7-VHH-C9BoNT-A

Plasmid

#202490

Purpose

Expresses the nanobody C9 BoNT/A with T7 induction

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody C9 BoNT/A

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pETM6-Amp-T7-GFPuv

Plasmid

#202463

Purpose

Expresses GFPuv with T7 induction

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

GFPuv

Expression

Bacterial

Promoter

T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAM211 His-Ubp6

Plasmid

#226359

Purpose

Expression of yeast Ubp6

Depositor

Andreas Martin

Article

Bashore et al Nat Struct Mol Biol. 2015 Sep;22(9):712-

Insert

ubp6 (UBP6 Budding Yeast)

Tags

His

Expression

Bacterial

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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MAC3-C-GFP-NLS

Plasmid

#222604

Purpose

Expression of MAC3-tagged GFP-NLS (C-terminal)

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

EGFP and 5' nuclear localizaition sequence (NLS) (PPKTTRKVED)

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 6, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC3-N-GFP-NES

Plasmid

#222606

Purpose

Expression of MAC3-tagged GFP-NES (N-terminal)

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

EGFP and 3' nuclear exclusion sequence (NES)

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC3-NTRK3

Plasmid

#222612

Purpose

Expression of MAC3-tagged NTRK3

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

NTRK3 (NTRK3 Human)

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC3-N-GFP-NLS

Plasmid

#222607

Purpose

Expression of MAC3-tagged GFP-NLS (N-terminal)

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

EGFP and 3' nuclear localizaition sequence (NLS)

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC3-C-GFP-NES

Plasmid

#222603

Purpose

Expression of MAC3-tagged GFP-NES (C-terminal)

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

EGFP and 5' nuclear exclusion sequence (NES)

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC3-N-GFP-Myr

Plasmid

#222608

Purpose

Expression of MAC3-tagged GFP-Myr (N-terminal)

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

EGFP and 3' myristoylation sequence (Myr)

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC3-C-GFP-Myr

Plasmid

#222605

Purpose

Expression of MAC3-tagged GFP-Myr (C-terminal)

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

EGFP and 5' myristoylation sequence (Myr)

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pSCL194

Plasmid

#222225

Purpose

Expresses site 1/Cas9/Eco1 RT in HIS3 locus.

Depositor

Seth Shipman

Article

Crawford et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

site 1/Cas9/Eco1 RT

Use

CRISPR

Expression

Yeast

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pSCL195

Plasmid

#222226

Purpose

Expresses site 2/Cas9/Eco1 RT in HIS3 locus.

Depositor

Seth Shipman

Article

Crawford et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

site 2/Cas9/Eco1 RT

Use

CRISPR

Expression

Yeast

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pSCL368

Plasmid

#222227

Purpose

Expresses site 3/Cas9/Eco1 RT in HIS3 locus.

Depositor

Seth Shipman

Article

Crawford et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

site 3/Cas9/Eco1 RT

Use

CRISPR

Expression

Yeast

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pET3a-Xl-H3∆Nterm[R42]

Plasmid

#221201

Purpose

Xenopus laevis histone H3 with N-terminal tail deleted.

Depositor

Gregory Bowman

Article

Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

Insert

Xl-H3∆Nterm[R42]

Expression

Bacterial

Available Since

July 11, 2024

Availability

Academic Institutions and Nonprofits only

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pPtet-1_mNeonGreen_Ptet-2_mCherry

Plasmid

#213839

Purpose

Expression of mNeonGreen and mCherry from aTc-inducible Ptet promoters.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen, mCherry

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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SNAPf-BASU-His

Plasmid

#218450

Purpose

E coli expression vector for SNAPf-BASU-His biotin ligase

Depositor

Thorsten Stafforst

Article

Hanswillemenke et al Nat Chem Biol. 2024 Jan 17. doi: 10.1038

Insert

SNAPf-BASU-His fusion protein

Expression

Bacterial

Promoter

T7

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC25A19

Plasmid

#166209

Purpose

Gateway entry vector for N. furzeri SLC25A19

Depositor

Daniel Jarosz

Article

Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

Insert

SLC25A19

Use

Gateway entry vector

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-SMPDL3B

Plasmid

#166211

Purpose

Gateway entry vector for N. furzeri SMPDL3B

Depositor

Daniel Jarosz

Article

Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

Insert

SMPDL3B

Use

Gateway entry vector

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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