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EcM2.1

Bacterial Strain

#64052

Purpose

Optimized strain for high-efficiency genome manipulations using CoS-MAGE and tolC cycling

Depositor

George Church

Article

Gregg et al Nucleic Acids Res. 2014 Apr;42(7):4779-9

Bacterial Resistance

Ampicillin

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
S4197

Bacterial Strain

#200839

Purpose

wildtype strain MG1655 rph+, ilvG+, ΔlacZ

Depositor

Karin Schnetz

Article

Venkatesh et al J Bacteriol. 2010 Dec;192(24):6456-64. d

Bacterial Resistance

None

Species

Escherichia coli str. k-12 substr. mg1655

Available Since

July 25, 2023

Availability

Academic Institutions and Nonprofits only
EcM2.1.dtolC

Bacterial Strain

#64053

Purpose

Optimized strain for high-efficiency genome manipulations using CoS-MAGE and tolC cycling

Depositor

George Church

Article

Gregg et al Nucleic Acids Res. 2014 Apr;42(7):4779-9

Bacterial Resistance

Ampicillin

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
EcM2.1 dtolC bla::zeoR

Bacterial Strain

#64054

Purpose

Optimized strain for high-efficiency genome manipulations using CoS-MAGE and tolC cycling

Depositor

George Church

Article

Gregg et al Nucleic Acids Res. 2014 Apr;42(7):4779-9

Bacterial Resistance

Bleocin (Zeocin)

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
BL21 Rosetta2 ΔrecBCD

Bacterial Strain

#176583

Purpose

E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 plasmid.

Depositor

Jean-Loup Faulon

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Bacterial Resistance

Chloramphenicol

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only
BL21 ΔrecBCD

Bacterial Strain

#176581

Purpose

E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression.

Depositor

Chase Beisel, Jean-Loup Faulon

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Bacterial Resistance

None

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only
BL21 Rosetta2 ΔrecB

Bacterial Strain

#176582

Purpose

E. coli BL21 strain (with recB genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 plasmid.

Depositor

Jean-Loup Faulon

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Bacterial Resistance

Chloramphenicol

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only
BL21 ΔrecB

Bacterial Strain

#176580

Purpose

E. coli BL21 strain (with recB genomic locus deleted) for making cell-free extracts for Linear DNA expression.

Depositor

Chase Beisel, Jean-Loup Faulon

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Bacterial Resistance

None

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only
ML14

Bacterial Strain

#61911

Purpose

C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. tyrA gene deleted.

Depositor

Robert Gennis, Toshio Iwasaki

Article

Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

Bacterial Resistance

None

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
ML17

Bacterial Strain

#61912

Purpose

C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. glnA gene deleted.

Depositor

Robert Gennis, Toshio Iwasaki

Article

Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

Bacterial Resistance

None

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
RF6

Bacterial Strain

#62074

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. proC gene deleted.

Depositor

Robert Gennis, Toshio Iwasaki

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
C321.∆A.opt

Bacterial Strain

#87359

Purpose

This is C321.∆A with additional mutations engineered by Kuznetsov et al. to improve doubling time.

Depositor

George Church

Article

Kuznetsov et al Genome Biol. 2017 May 25;18(1):100. doi:

Bacterial Resistance

Gentamicin

Species

Synthetic

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only
RF1

Bacterial Strain

#61962

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. glyA gene deleted

Depositor

Robert Gennis, Toshio Iwasaki

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
RF2

Bacterial Strain

#62070

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. thrC gene deleted

Depositor

Robert Gennis, Toshio Iwasaki

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
BL21 Rosetta2 ΔrecBCD GamS

Bacterial Strain

#176586

Purpose

E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids.

Depositor

Jean-Loup Faulon

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Bacterial Resistance

Chloramphenicol and Ampicillin

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only
RF11

Bacterial Strain

#61961

Purpose

C43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. metA gene deleted

Depositor

Robert Gennis, Toshio Iwasaki

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
YM138

Bacterial Strain

#61953

Purpose

C43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. cysE gene deleted.

Depositor

Robert Gennis, Toshio Iwasaki

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
RF10

Bacterial Strain

#62076

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. lysA genes deleted.

Depositor

Robert Gennis, Toshio Iwasaki

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
ML21

Bacterial Strain

#61914

Purpose

C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. hisG tyrA genes deleted.

Depositor

Robert Gennis, Toshio Iwasaki

Article

Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

Bacterial Resistance

None

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
RF8

Bacterial Strain

#62075

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. asnA asnB genes deleted.

Depositor

Robert Gennis, Toshio Iwasaki

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only

Showing: 1 - 20 of 99 results

Results per page:

Sort results by:

We narrowed to 99 results for: ache

EcM2.1

S4197

EcM2.1.dtolC

EcM2.1 dtolC bla::zeoR

BL21 Rosetta2 ΔrecBCD

BL21 ΔrecBCD

BL21 Rosetta2 ΔrecB

BL21 ΔrecB

ML14

ML17

RF6

C321.∆A.opt

RF1

RF2

BL21 Rosetta2 ΔrecBCD GamS

RF11

YM138

RF10

ML21

RF8

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