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Showing: 1 - 20 of 881 results
  1. Gersbach Lab CRISPR Plasmids

    Type
    Collection
    ... CRISPR Gersbach Lab CRISPR Plasmids Gersbach Lab CRISPR Plasmids ...plasmids from Dr. Charles Gersbach's lab. CRISPR...DG, Leong KW, Guilak F, Crawford GE, Reddy TE, Gersbach CA. Nat. Methods . 2013 July. PubMed PMID 23892895...lentiviral vector. Kabadi AM, Ousterout DG, Hilton IB, Gersbach CA. Nucleic Acids Res. . 2014. PubMed PMID 25122746...
  2. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Description Article PI Acetylcholine Family of ACh sensors (GACh) based on G-protein-coupled receptors A genetically...sensor for monitoring in vivo cholinergic activity (GACh3.0) (Constitutive or Cre-dependent) An optimized acetylcholine...Fluorescent sensor for in vivo acetylcholine detection (iAchSnFR) (Constitutive or Cre-dependent) A fast genetically...a Selective and Sensitive Serotonin Sensor via Machine Learning. Cell. 2020 Dec 12. pii: S0092-8674(20... A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters. Nat ...Positron (Constitutive or Cre-dependent) A general approach to engineer positive-going eFRET voltage indicators...
  3. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...colonies, mini-prep each one, and subject each clone to Sanger sequencing to characterize each deletion allele...mini-prep culture. Perform mini-prep for each sample and sequence each colony using a U6 promoter forward primer...steps in step 7 . Transfer 50 μl from each well (leaving 100 μl in each well) to a 96-well PCR plate using...trypsin-EDTA to each well with a clone present. Resuspend cells in 200 μl of media. Pipette mix to detach cells....other plate to screen each clone for deletions. Add an additional 100 μl to each well for a total volume...utility for the study of non-coding elements. This approach can be used for efficient loss-of-function studies...bottom strand. Determine the reverse complement of each guide sequence. Example reverse complement sequences...
  4. Antibody Guide

    Type
    Collection
    ...applications common to each category, including an outline of the basic steps needed for each application, important...different isotypes for each primary antibody and secondary antibodies specific to each isotype. Overview of... data. Check that your machine has appropriate lasers and filters for each fluorophore you select. Suggested... itself prior to antibody production; therefore, each B cell only produces one type of antibody. Monocultures...enable higher production of a single antibody, but each B cell can only produce one variable region. Isotypes...-antibody units, while others exist as monomers. Each antibody class therefore has a valency, or number...typically the number of antibodies multiplied by two, as each antibody has two binding sites. Isotype Description...
  5. CRISPR Guide

    Type
    Collection
    ...specificity. Dual-nickase approach increases specificity but is less efficient. Each putative knockout allele...engineering . Prior to CRISPR, genome engineering approaches like zinc finger nucleases (ZFNs) or transcription-activator-like...rapidly become the most popular genome engineering approach. Engineered CRISPR systems contain two components...experiment, you might consider using the dual nickase approach to create a double nick-induced DSB. The nickase...have been engineered using cell-based selection approaches. Yeast-based screening systems resulted in evolved...termed left & right homology arms .) The length of each homology arm is dependent on the size of the change...several gRNAs from the same plasmid ensures that each cell containing the plasmid will express all of ...
  6. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...two homology arms on each side flanking your desired insertion or mutation. Each arms is around 800bp....two homology arms on each side flanking your desired insertion or mutation. Each arm is around 800bp. ...is to co-transfect several plasmid together, with each plasmid having a targeting spacer cloned into the...genomic loci with wildtype Cas9, clone two spacers for each locus into PX330, then co-transfect or delivery ...with PX335 vector, you need to find two guides for each cleavage site, but this system probably have better...Typical design is to have 50-80bp of homology arms on each side flanking the change you'd like to introduce...transfection, you could use 1ul of 10uM oligo stock for each well on 24-well plate, co-deliver into the cells...
  7. Michael Davidson Fluorescent Protein Collection

    Type
    Collection
    ...Description Industry Attachments mEmeralds Plasmid Description Industry Attachments Reds Plasmid Description...Description Industry Attachments Blues Plasmid Description Industry Attachments Cyans Plasmid Description ...Description Industry Attachments Oranges Plasmid Description Industry Attachments Greens Plasmid Description Industry...Industry Attachments Yellows Plasmid Description Industry Attachments Photoactivatables Plasmid Description...Description Industry Attachments Do you have suggestions for other plasmids that should be added to this list...
  8. Fluorescent Protein Guide: FRET

    Type
    Collection
    ... amino acid linker mGFP-10-sREACh-N3 Monomeric EGFP attached to super-REACh via a 10 amino acid linker...Pair C5V Cerulean attached to Venus via a 5 amino acid linker C17V Cerulean attached to Venus via a 17...linker C32V Cerulean attached to Venus via a 32 amino acid linker C5A Cerulean attached to Amber via a 5 ...linker C17A Cerulean attached to Amber via a 17 amino acid linker C32A Cerulean attached to Amber via a 32... to study (1) protein-protein interactions where each protein is separately fused to a donor or acceptor...
  9. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...MDH-related research into their teaching laboratories. They encourage instructors teaching different types of courses...projects, find teaching resources and protocols, learn about regional hubs, access a teaching mentor, and...development, membership to a local hub of educators teaching MDH CUREs, and opportunities to mentor future ...Clone Information Sheet, available to download from each plasmid page. Use the search bar or sort buttons...and more. References for teachers Using Bioinformatics and Molecular Visualization to Develop Student Hypotheses...
  10. Deisseroth INTRSECT Collection

    Type
    Collection
    ...proof-of-concept targeting approach in 2014 1 (using EYFP and ChR2-EYFP as payloads). This approach has been broadly...137145 pAAV-nEF-Con/Fon-bREACHes-EYFP Cre AND Flp 137146 pAAV-nEF-Con/Foff 2.0-bREACHes-EYFP Cre AND NOT Flp...gene or projection). Intersectional expression approaches combine multiplexed recombinases, including Cre...Flp FRT/F5 137147 pAAV-nEF-Coff/Fon-bREACHes-EYFP Flp AND NOT Cre 137158 pAAV-nEF-ChRmine-mScarlet None...PMID:24908100 Chuhma N, Mingote S, Yetnikoff L, Kalmbach A, Ma T, Ztaou S, Sienna AC, Tepler S, Poulin ... neuron subtypes using intersectional genetic approaches. Nat Neurosci. 21(9):1260-1271. PMID:30104732...
  11. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... none S. pyogenes Gersbach pmU6-gRNA 53187 Mammalian BbsI none S. pyogenes Gersbach phU6-gRNA 53188 Mammalian... none S. pyogenes Gersbach ph7SK-gRNA 53189 Mammalian BbsI none S. pyogenes Gersbach PX552 60958 Mammalian... pSPgRNA 47108 Mammalian BbsI none S. pyogenes Gersbach pUC57-sgRNA expression vector 51132 Mammalian ...gRNA-hU6 61366 Mammalian U6 none N. meningitidis Gersbach pSPneogRNAH 63556 Other/Leishmania none S. pyogenes...Lentiviral hU6 yes, interfere S. pyogenes Puro Gersbach pHEE401 71286 Plant U6-26p yes, cut S. pyogenes...into one construct using Golden Gate assembly. Gersbach MuLE System Mammalian MultiSite Gateway recombination-based...Drosophila Utilizes endogenous tRNA processing machinery to excise multiple functional gRNAs from a single...
  12. Antibody Production

    Type
    Collection
    ...shipping conditions for each order based on the total items in the order, ensuring each item ships safely and...using standard methods individually optimized for each specific antibody in order to generate high quality...with >90% purity are distributed. Quality Control Each lot of antibody undergoes testing, either in-house...The specific QC experiments performed varies for each antibody catalog item. Whenever possible, the experiment...preparing single-use aliquots before storing at -20 °C. Each antibody catalog item is tested for shipping stability...
  13. AAVED

    Type
    Collection
    ...using combinations of AAV-based molecular tools to achieve targeted neuronal manipulation (aka intersectional...Harvard Medical School Dr. Melina Fan, Dr. Susanna Bachle, Dr. Karen Guerin, Dr. Leila Haery, Dr. Meghan ...:00 PM Adjourn Meeting Preparation For Speakers: Each speaker will give a 20 minute presentation, followed...Potential combination with other technologies to achieve different experimental goals. How does this method... (that your group is presenting on) is used to achieve targeted neuronal manipulation. Figures, tables...Woodard K, Cepko C, Guerin KI, Rego MA, Ersing I, Bachle SM, Kamens J, Fan M. Front Neuroanat. 2019 Nov ...
  14. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...Neuroligin-1 Rat Mouse IgG2a 114555 Anti-MMACHC [N289/16R] MMACHC Human Mouse IgG2a 114556 Anti-SUR1 [N212...NGL-1/LRRC4C Human Mouse IgG2a 182117 Anti-VAChT [N6/38R] VAChT Human Mouse IgG2a 182118 Anti-TrpC5 [N67...hormone receptor Mouse IgG2a 190326 Anti-VAChT [N425/45] VAChT Rat Mouse IgG2a 190327 Anti-ANO6/TMEM16F...potassium channel Rat Mouse IgG2b 206672 Anti-VAChT [N425/45R-2b] VAChT vesicular acetylcholine transporter Rat...Rat Mouse IgG2b 206673 Anti-VAChT [N6/38R-2b] VAChT Human Mouse IgG2b 206674 Anti-Nav1.7 Na+ channel [...glutamate receptor Rat Mouse IgG1 206716 Anti-VAChT [N6/38R-1] VAChT Human Mouse IgG1 206718 Anti-HCN1 ion channel... K+ channel Rat Mouse IgG1 211600 Anti-VAChT [N425/45R-1] VAChT Rat Mouse IgG1 Addgene distributes ready-to-use...
  15. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...titers are listed on each item's material page. Actual titers are reported with each shipment. Find Available... Vector Core catalog items. Virus associated DNA Each viral service request will also include virus associated...
  16. Serotype Testing AAV

    Type
    Collection
    ...expression from the CAG promoter. For information about each catalog item, including the titer and quality control...control, visit the material page (linked for each catalog ID below). Serotype Catalog ID Volume Price*...the human synpasin promoter. For information about each catalog item, including the titer and quality control...Retrograde 50465-AAVrg.T 20 µL $ 140 Add to Cart *Each viral service request will also include virus associated...
  17. Arf GTPase Family

    Type
    Collection
    ...family, information on each gene, and examples of plasmid types available for each gene. Click the gene ... GAP set is referred to as a minimal triad, with each component involved in GTPase signaling activity....
  18. Biosensor AAV Preps

    Type
    Collection
    ... 137950 pAAV.hSynap.iAChSnFR Syn iAChSnFR none Constitutive 1 Looger 137955 pAAV.CAG.iAChSnFR CAG iAChSnFR...Sensors HaloCaMP1a HaloCaMP1b Acetylcholine Sensors iAChSnFR Dopamine Sensors dLight RdLight GRAB_DA GRAB_rDA...
  19. mTOR Pathway

    Type
    Collection
    ... found in two complexes, mTORC1 and mTORC2. Each complex contains a unique subunit: raptor for...multiple isoforms or subunits, individual links to each gene page are provided below. Color is used for ...multiple isoforms or subunits, individual links to each gene page are provided below. mTORC1 Complex Symbol...PubMed PMID: 24508508 . Targeting the translation machinery in cancer. Bhat M, Robichaud N, Hulea L, Sonenberg...
Showing: 1 - 20 of 881 results