...Description Article PI Acetylcholine Family of ACh sensors (GACh) based on G-protein-coupled receptors A genetically...sensor for monitoring in vivo cholinergic activity (GACh3.0) (Constitutive or Cre-dependent) An optimized acetylcholine...Fluorescent sensor for in vivo acetylcholine detection (iAchSnFR) (Constitutive or Cre-dependent) A fast genetically...a Selective and Sensitive Serotonin Sensor via Machine Learning. Cell. 2020 Dec 12. pii: S0092-8674(20... A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters. Nat ...Positron (Constitutive or Cre-dependent) A general approach to engineer positive-going eFRET voltage indicators...

...colonies, mini-prep each one, and subject each clone to Sanger sequencing to characterize each deletion allele...mini-prep culture. Perform mini-prep for each sample and sequence each colony using a U6 promoter forward primer...steps in step 7 . Transfer 50 μl from each well (leaving 100 μl in each well) to a 96-well PCR plate using...trypsin-EDTA to each well with a clone present. Resuspend cells in 200 μl of media. Pipette mix to detach cells....other plate to screen each clone for deletions. Add an additional 100 μl to each well for a total volume...utility for the study of non-coding elements. This approach can be used for efficient loss-of-function studies...bottom strand. Determine the reverse complement of each guide sequence. Example reverse complement sequences...

...applications common to each category, including an outline of the basic steps needed for each application, important...different isotypes for each primary antibody and secondary antibodies specific to each isotype. Overview of...confusing data. Check that your machine has appropriate lasers and filters for each fluorophore you select. Suggested... itself prior to antibody production; therefore, each B cell only produces one type of antibody. Monocultures...enable higher production of a single antibody, but each B cell can only produce one variable region. Isotypes...-antibody units, while others exist as monomers. Each antibody class therefore has a valency, or number...typically the number of antibodies multiplied by two, as each antibody has two binding sites. Isotype Description...

...technologies. Before CRISPR, genome engineering approaches like zinc finger nucleases (ZFNs) or transcription-activator-like...rapidly became the most popular genome engineering approach. Figure 1: Overview of the basic CRISPR mechanism...the ways the CRISPR components are leveraged to achieve specific goals, such as targeting multiple genomic...gRNAs into a single plasmid — though some have achieved targeting in the double digits. These multiplex...nickase system is utilized in many CRISPR systems to achieve specific genetic edits. The dCas9 molecule binds...termed left and right homology arms). The length of each homology arm depends on the size of the change being...system, the Cas effectors lack nuclease activity. Each system also includes Tn7-like transposase components...

...two homology arms on each side flanking your desired insertion or mutation. Each arms is around 800bp....two homology arms on each side flanking your desired insertion or mutation. Each arm is around 800bp. ...is to co-transfect several plasmid together, with each plasmid having a targeting spacer cloned into the...genomic loci with wildtype Cas9, clone two spacers for each locus into PX330, then co-transfect or delivery ...with PX335 vector, you need to find two guides for each cleavage site, but this system probably have better...Typical design is to have 50-80bp of homology arms on each side flanking the change you'd like to introduce...transfection, you could use 1ul of 10uM oligo stock for each well on 24-well plate, co-deliver into the cells...

... amino acid linker mGFP-10-sREACh-N3 Monomeric EGFP attached to super-REACh via a 10 amino acid linker...Pair C5V Cerulean attached to Venus via a 5 amino acid linker C17V Cerulean attached to Venus via a 17...linker C32V Cerulean attached to Venus via a 32 amino acid linker C5A Cerulean attached to Amber via a 5 ...linker C17A Cerulean attached to Amber via a 17 amino acid linker C32A Cerulean attached to Amber via a 32... to study (1) protein-protein interactions where each protein is separately fused to a donor or acceptor...

...MDH-related research into their teaching laboratories. They encourage instructors teaching different types of courses...projects, find teaching resources and protocols, learn about regional hubs, access a teaching mentor, and...development, membership to a local hub of educators teaching MDH CUREs, and opportunities to mentor future ...Clone Information Sheet, available to download from each plasmid page. Use the search bar or sort buttons...and more. References for teachers Using Bioinformatics and Molecular Visualization to Develop Student Hypotheses...

...proof-of-concept targeting approach in 2014 1 (using EYFP and ChR2-EYFP as payloads). This approach has been broadly... pAAV-nEF-Con/Fon-bREACHes-EYFP Cre AND Flp No 137146 pAAV-nEF-Con/Foff 2.0-bREACHes-EYFP Cre AND NOT ...gene or projection). Intersectional expression approaches combine multiplexed recombinases, including Cre...NOT Flp FRT/F5 No 137147 pAAV-nEF-Coff/Fon-bREACHes-EYFP Flp AND NOT Cre No 137158 pAAV-nEF-ChRmine-mScarlet... new window) Chuhma N, Mingote S, Yetnikoff L, Kalmbach A, Ma T, Ztaou S, Sienna AC, Tepler S, Poulin ... neuron subtypes using intersectional genetic approaches. Nat Neurosci. 21(9):1260-1271. PubMed (Link ...

... 137950 pAAV.hSynap.iAChSnFR Syn iAChSnFR none Constitutive 1 Looger 137955 pAAV.CAG.iAChSnFR CAG iAChSnFR...HaloCaMP1b NIR-GECO Acetylcholine Sensors iAChSnFR GRAB_ACh Dopamine Sensors dLight RdLight GRAB_DA GRAB_rDA...Constitutive 1, 9 Looger 121922 pAAV-hSyn-GRAB_ACh3.0 Syn GRAB_ACh3.0 none Constitutive 9 Li Calcium Sensor...

... none S. pyogenes Gersbach pmU6-gRNA 53187 Mammalian BbsI none S. pyogenes Gersbach phU6-gRNA 53188 Mammalian... none S. pyogenes Gersbach ph7SK-gRNA 53189 Mammalian BbsI none S. pyogenes Gersbach PX552 60958 Mammalian... pSPgRNA 47108 Mammalian BbsI none S. pyogenes Gersbach pUC57-sgRNA expression vector 51132 Mammalian ...gRNA-hU6 61366 Mammalian U6 none N. meningitidis Gersbach pSPneogRNAH 63556 Other/Leishmania none S. pyogenes...Lentiviral hU6 yes, interfere S. pyogenes Puro Gersbach pHEE401 71286 Plant U6-26p yes, cut S. pyogenes...into one construct using Golden Gate assembly. Gersbach MuLE System Mammalian MultiSite Gateway recombination-based...Drosophila Utilizes endogenous tRNA processing machinery to excise multiple functional gRNAs from a single...

...shipping conditions for each order based on the total items in the order, ensuring each item ships safely and...using standard methods individually optimized for each specific antibody in order to generate high quality...with >90% purity are distributed. Quality Control Each lot of antibody undergoes testing, either in-house...The specific QC experiments performed varies for each antibody catalog item. Whenever possible, the experiment...preparing single-use aliquots before storing at -20 °C. Each antibody catalog item is tested for shipping stability...

...specificity. Dual-nickase approach increases specificity but is less efficient. Each putative knockout allele...reagents may be sufficient to express the CRISPR machinery. For more difficult cells (e.g. primary cells)...result in reduced cleavage. The number of alleles for each gene may vary depending on the specific cell line...essential protein domains. The benefit of this approach is that even non-frameshift alleles may alter ...Furthermore, in cells containing two mutated alleles, each mutated allele may be different owing to the error-prone... examine off-target effects. More information on each of these techniques can be found in our blog post...

...using combinations of AAV-based molecular tools to achieve targeted neuronal manipulation (aka intersectional...Harvard Medical School Dr. Melina Fan, Dr. Susanna Bachle, Dr. Karen Guerin, Dr. Leila Haery, Dr. Meghan ...:00 PM Adjourn Meeting Preparation For Speakers: Each speaker will give a 20 minute presentation, followed...Potential combination with other technologies to achieve different experimental goals. How does this method... (that your group is presenting on) is used to achieve targeted neuronal manipulation. Figures, tables...Woodard K, Cepko C, Guerin KI, Rego MA, Ersing I, Bachle SM, Kamens J, Fan M. Front Neuroanat. 2019 Nov ...

...Neuroligin-1 Rat Mouse IgG2a 114555 Anti-MMACHC [N289/16R] MMACHC Human Mouse IgG2a 114556 Anti-SUR1 [N212...NGL-1/LRRC4C Human Mouse IgG2a 182117 Anti-VAChT [N6/38R] VAChT Human Mouse IgG2a 182118 Anti-TrpC5 [N67...hormone receptor Mouse IgG2a 190326 Anti-VAChT [N425/45] VAChT Rat Mouse IgG2a 190327 Anti-ANO6/TMEM16F...+ channel Rat Mouse IgG2b 206672 Anti-VAChT [N425/45R-2b] VAChT vesicular acetylcholine transporter Rat...Rat Mouse IgG2b 206673 Anti-VAChT [N6/38R-2b] VAChT Human Mouse IgG2b 206674 Anti-Nav1.7 Na+ channel [...glutamate receptor Rat Mouse IgG1 206716 Anti-VAChT [N6/38R-1] VAChT Human Mouse IgG1 206717 Anti-Nav1.7 Na+... K+ channel Rat Mouse IgG1 211600 Anti-VAChT [N425/45R-1] VAChT Rat Mouse IgG1 219442 Anti-Kv1.1 K+ channel...

...collection of 20 CRISPR plasmids. Each point represents a single cell, and each color represents the population...expression levels. This collection comprises 20 plasmids, each carrying two CRISPR guides. The first one binds ...integration of the aTc-inducible pTet-dCas9 in the attachment site of the phage 186. Detailed information about...promoter integrated in the chromosome at phage attachment sites. The levels of the two reporters, mCherry...

...expression from the CAG promoter. For information about each catalog item, including the titer and quality control...control, visit the material page (linked for each catalog ID below). Serotype Catalog ID Volume Price*...the human synpasin promoter. For information about each catalog item, including the titer and quality control...Retrograde 50465-AAVrg.T 20 µL $ 140 Add to Cart *Each viral service request will also include virus associated...

...family, information on each gene, and examples of plasmid types available for each gene. Click the gene ... GAP set is referred to as a minimal triad, with each component involved in GTPase signaling activity....

...sensitivity, wide dynamic range, and resistance to photobleaching has made luciferase a common choice in assays...optimized ( CBR2opt ) for mammalian expression systems. Each type of luciferase has advantages and disadvantages...luciferase desined de novo using deep-learning approaches. Split-Luciferase and Protein-Protein Interaction... using Gateway cloning. Use with 174051 Alberto Macho 174051 pGWB-cLUC Firefly Creation of C-terminal ... using Gateway cloning. Use with 174050 Alberto Macho 136010 psi-CHECK3 Firefly/Renilla Insertion of 3...

... found in two complexes, mTORC1 and mTORC2. Each complex contains a unique subunit: raptor for...multiple isoforms or subunits, individual links to each gene page are provided below. Color is used for ...multiple isoforms or subunits, individual links to each gene page are provided below. mTORC1 Complex Symbol...PubMed PMID: 24508508 . Targeting the translation machinery in cancer. Bhat M, Robichaud N, Hulea L, Sonenberg...

..., TK25 FGFR3 fibroblast growth factor receptor 3 ACH, CD333, CEK2, HSFGFR3EX, JTK4 FGFR4 fibroblast growth...table for each category. The gene names in these tables link to the available plasmids for each gene. Please...STC-2, STCRP TAC1 tachykinin, precursor 1 Hs.2563, NK2, NKNA, NPK, TAC2 TACR1 tachykinin receptor 1 NK1R...organisms are exposed to millions of potential pathogens each day. Their ability to protect against infection ...enable cells of the immune system to communicate with each other across long distances. During infection and... these tables link to the available plasmids for each gene. Please note that not all genes have plasmids...PIG4, SAA, TP53I4 SAA2 serum amyloid A2 - SBDS Shwachman-Bodian-Diamond syndrome CGI-97, FLJ10917, SDS,...