We narrowed to 958 results for: ache

...Description Article PI Acetylcholine Family of ACh sensors (GACh) based on G-protein-coupled receptors A genetically...sensor for monitoring in vivo cholinergic activity (GACh3.0) (Constitutive or Cre-dependent) An optimized acetylcholine...Fluorescent sensor for in vivo acetylcholine detection (iAchSnFR) (Constitutive or Cre-dependent) A fast genetically...a Selective and Sensitive Serotonin Sensor via Machine Learning. Cell. 2020 Dec 12. pii: S0092-8674(20... A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters. Nat ...Positron (Constitutive or Cre-dependent) A general approach to engineer positive-going eFRET voltage indicators...

...applications common to each category, including an outline of the basic steps needed for each application, important...different isotypes for each primary antibody and secondary antibodies specific to each isotype. Overview of...confusing data. Check that your machine has appropriate lasers and filters for each fluorophore you select. Suggested... itself prior to antibody production; therefore, each B cell only produces one type of antibody. Monocultures...enable higher production of a single antibody, but each B cell can only produce one variable region. Isotypes...-antibody units, while others exist as monomers. Each antibody class therefore has a valency, or number...typically the number of antibodies multiplied by two, as each antibody has two binding sites. Isotype Description...

...technologies. Before CRISPR, genome engineering approaches like zinc finger nucleases (ZFNs) or transcription-activator-like...rapidly became the most popular genome engineering approach. Engineered CRISPR systems contain two components...the ways the CRISPR components are leveraged to achieve specific goals, such as targeting multiple genomic...gRNAs into a single plasmid — though some have achieved targeting in the double digits. These multiplex...nickase system is utilized in many CRISPR systems to achieve specific genetic edits. The dCas9 molecule binds...termed left and right homology arms). The length of each homology arm depends on the size of the change being...system, the Cas effectors lack nuclease activity. Each system also includes Tn7-like transposase components...

...mTurquoise2 sREACh** (super-REACh, aka Nữ) 434 0.93 531** 115,000 0.07 6.1 3.0 pmTurquoise2-N1 , sREACh-C1 , ..., sReach-mTurquoise2 EGFP sREACh** (super-REACh, aka Nữ) 488 0.60 531** 115,000 0.07 6.0 4.1 mEGFP-N1 ...mEGFP-N1 , sREACh-C1 , mGFP-10-sREACh-N3 EGFP ShadowY** 488 0.6 531** 136,000 0.01 6.1 4.5 mEGFP-N1 , CMV-ShadowY... to study (1) protein-protein interactions where each protein is separately fused to a donor or acceptor...

...MDH-related research into their teaching laboratories. They encourage instructors teaching different types of courses...projects, find teaching resources and protocols, learn about regional hubs, access a teaching mentor, and...development, membership to a local hub of educators teaching MDH CUREs, and opportunities to mentor future ...Clone Information Sheet, available to download from each plasmid page. Use the search bar or sort buttons...and more. References for teachers Using Bioinformatics and Molecular Visualization to Develop Student Hypotheses...

...proof-of-concept targeting approach in 2014 1 (using EYFP and ChR2-EYFP as payloads). This approach has been broadly... pAAV-nEF-Con/Fon-bREACHes-EYFP Cre AND Flp No 137146 pAAV-nEF-Con/Foff 2.0-bREACHes-EYFP Cre AND NOT ...gene or projection). Intersectional expression approaches combine multiplexed recombinases, including Cre...NOT Flp FRT/F5 No 137147 pAAV-nEF-Coff/Fon-bREACHes-EYFP Flp AND NOT Cre No 137158 pAAV-nEF-ChRmine-mScarlet... new window) Chuhma N, Mingote S, Yetnikoff L, Kalmbach A, Ma T, Ztaou S, Sienna AC, Tepler S, Poulin ... neuron subtypes using intersectional genetic approaches. Nat Neurosci. 21(9):1260-1271. PubMed (Link ...

...specificity Dual-nickase approach increases specificity but is less efficient Each putative knockout allele... strategies for designing the gRNA component for each category. Genetic Manipulation Application Cas Enzyme...SAM system Activate Design and Clone Your gRNA Achieving your desired edit comes down to location, location...transient or stable). For plasmid and viral-based approaches, the species and expression pattern of the promoter...cationic lipids, electroporation, or an endosomal approach. Similar to the mRNA method described above, this...Furthermore, in cells containing two mutated alleles, each mutated allele may be different owing to the error-prone...verify that your cells contain your desired edit. Each of these methods has their own considerations. Which...

...shipping conditions for each order based on the total items in the order, ensuring each item ships safely and...using standard methods individually optimized for each specific antibody in order to generate high quality...with >90% purity are distributed. Quality Control Each lot of antibody undergoes testing, either in-house...The specific QC experiments performed varies for each antibody catalog item. Whenever possible, the experiment...preparing single-use aliquots before storing at -20 °C. Each antibody catalog item is tested for shipping stability...

...137950 pAAV.hSynap.iAChSnFR Syn iAChSnFR none Constitutive 1 Loren Looger 137955 pAAV.CAG.iAChSnFR CAG iAChSnFR... iAChSnFR none Constitutive 1, 9 Loren Looger 121922 pAAV-hSyn-GRAB_ACh3.0 Syn GRAB_ACh3.0 none Constitutive...HaloCaMP1b NIR-GECO Acetylcholine Sensors iAChSnFR GRAB_ACh Adenosine Sensors GRAB Ado Dopamine Sensors...

...Neuroligin-1 Rat Mouse IgG2a 114555 Anti-MMACHC [N289/16R] MMACHC Human Mouse IgG2a 114556 Anti-SUR1 [N212...NGL-1/LRRC4C Human Mouse IgG2a 182117 Anti-VAChT [N6/38R] VAChT Human Mouse IgG2a 182118 Anti-TrpC5 [N67...hormone receptor Mouse IgG2a 190326 Anti-VAChT [N425/45] VAChT Rat Mouse IgG2a 190327 Anti-ANO6/TMEM16F...+ channel Rat Mouse IgG2b 206672 Anti-VAChT [N425/45R-2b] VAChT vesicular acetylcholine transporter Rat...Rat Mouse IgG2b 206673 Anti-VAChT [N6/38R-2b] VAChT Human Mouse IgG2b 206674 Anti-Nav1.7 Na+ channel [...glutamate receptor Rat Mouse IgG1 206716 Anti-VAChT [N6/38R-1] VAChT Human Mouse IgG1 206717 Anti-Nav1.7 Na+... K+ channel Rat Mouse IgG1 211600 Anti-VAChT [N425/45R-1] VAChT Rat Mouse IgG1 219442 Anti-Kv1.1 K+ channel...

...collection of 20 CRISPR plasmids. Each point represents a single cell, and each color represents the population...expression levels. This collection comprises 20 plasmids, each carrying two CRISPR guides. The first one binds ...integration of the aTc-inducible pTet-dCas9 in the attachment site of the phage 186. Detailed information about...promoter integrated in the chromosome at phage attachment sites. The levels of the two reporters, mCherry...

...expression from the CAG promoter. For information about each catalog item, including the titer and quality control...control, visit the material page (linked for each catalog ID below). Serotype Catalog ID Volume Price*...the human synpasin promoter. For information about each catalog item, including the titer and quality control...Retrograde 50465-AAVrg.T 20 µL $ 150 Add to Cart *Each viral service request will also include virus associated...

...sensitivity, wide dynamic range, and resistance to photobleaching have made luciferase a popular tool for scientists... Cypridina luciferase , and Luciola luciferase . Each type of luciferase has advantages and disadvantages... using Gateway cloning. Use with 174051 Alberto Macho 174051 pGWB-cLUC Firefly Creation of C-terminal ... using Gateway cloning. Use with 174050 Alberto Macho 136010 psi-CHECK3 Firefly, Renilla Insertion of ...luciferases desined de novo using deep-learning approaches. Split Luciferase Complementation assays can ...fragments for protein-protein interaction assays. Each target protein is fused to either LgBiT or SmBiT...

...applications as vaccines. Selecting a Viral Vector Type Each viral vector type exhibits different properties ...cells in an organism and use the host cellular machinery to produce more virions. B) Viral plasmids have...to packaging cell lines and use their cellular machinery to generate viral vectors for research and gene...viral genome contains only the necessary genes to achieve this goal. When a virus recognizes a host cell,...genome into the cytoplasm and hijacks the host’s machinery to produce more viruses. In this way, viruses ...

...family, information on each gene, and examples of plasmid types available for each gene. Click the gene ... GAP set is referred to as a minimal triad, with each component involved in GTPase signaling activity....

...simplicity, other promoter elements and transcription machinery are not shown. Tetracycline Off (Tet-Off) The ...doxycycline (Zhou et al., 2006). Tet-On 3G systems can achieve >10,000-fold induction of the gene of interest....cell lines or transgenic animals can be made to achieve greater control over expression with flexibility... of interest from one plasmid. A single vector approach may not have as much flexibility and control as...lineages and transcriptional and proliferative state of each cell in a population. Proliferation history is monitored...7792603 (Link opens in a new window) Heinz, N., Schambach, A., Galla, M., Maetzig, T., Baum, C., Loew, R...

...expression of specific genes in cells or organisms. Each recombinase enzyme recognizes and binds pairs of... or translocation) depends on the orientation of each target site (see Figure 1). Figure 1: Recombination...Experimental Considerations Recombinase Recognition Sites Each enzyme functions analogously but interacts only ...tetracycline-regulated or other drug-inducible approach may also be used. Split proteins: The recombinase... for Site-specific Recombination in Drosophila Teaching an Old DOG New Tricks: Controlling Protein Activity...

...tissue, these protein barcodes allow for efficient machine-learning segmentation of neurons and proofreading...packaging representation. To read out the barcodes, each protein tag can be imaged with a corresponding primary...been used in expansion microscopy workflows to achieve high-resolution optical imaging down to individual... microscopy and reconstruction of neurons with machine learning. Adapted from Park & Sheridan et al. (...

... found in two complexes, mTORC1 and mTORC2. Each complex contains a unique subunit: raptor for...multiple isoforms or subunits, individual links to each gene page are provided below. Color is used for ...multiple isoforms or subunits, individual links to each gene page are provided below. mTORC1 Complex Symbol...PubMed PMID: 24508508 . Targeting the translation machinery in cancer. Bhat M, Robichaud N, Hulea L, Sonenberg...

..., TK25 FGFR3 fibroblast growth factor receptor 3 ACH, CD333, CEK2, HSFGFR3EX, JTK4 FGFR4 fibroblast growth...table for each category. The gene names in these tables link to the available plasmids for each gene. Please...STC-2, STCRP TAC1 tachykinin, precursor 1 Hs.2563, NK2, NKNA, NPK, TAC2 TACR1 tachykinin receptor 1 NK1R...organisms are exposed to millions of potential pathogens each day. Their ability to protect against infection ...enable cells of the immune system to communicate with each other across long distances. During infection and... these tables link to the available plasmids for each gene. Please note that not all genes have plasmids...PIG4, SAA, TP53I4 SAA2 serum amyloid A2 - SBDS Shwachman-Bodian-Diamond syndrome CGI-97, FLJ10917, SDS,...