...Janknecht 1991, Hochuli 1988). In this purification approach, the metal ions are immobilized on a bead or resin...Bannwarth W, Döbeli H, Gentz R and Stüber D. Genetic Approach to Facilitate Purification of Recombinant Proteins...

...their career choices.” She points out that a PhD teaches analytical and critical thinking skills that are...on to work in patent law, business development, teaching, etc. Church also encourages his mentees to follow...passion True success evolves from heart and hustle. Each PI emphasizes the importance of passion, hard work...

...teams of 4 people face each other on a single chosen clinical disease case. Then, each team received a whole...planarian tools for teaching developmental biology Learn how BioBuilder can help teach students about Synthetic...courses on genetic manipulation more hands on While teaching courses about gene therapy and genetic manipulation...knowledge to solve a clinical disease case. As a teacher, you explain the game rules and then you let your...Transfer Plasmid” game board. Once they agreed on each element to use, they had to place the selected card... 10 minutes to expose their strategy, justifying each single element used to build the construct and explain...practice, so it is based on a win-win strategy. After each team presented, all students had to pick the best...

...specific cells, but there are too few resources to teach students how to perform these experiments in the...different genes cloned into plasmids to investigate for each of these planarian species. These plasmids all have... through physical blocking of the translation machinery. In the Sánchez lab protocol, dsRNAs are delivered...Alejandro Sánchez Alvarado, The American Biology Teacher, Vol. 79 No. 3, March 2017; (pp. 208-223). Link...

... can a coach do that is different from an adviser, boss or mentor? First, professional coaches have experience...of the benefits of coaching relationships and do not know where to look for a coach. Many (most?) graduate.... However most coaches who focus on scientists are sensitive to this and have coaching plans that are ...find a coach? Start with referrals. Are there scientists you know who have had a successful coaching experience... assess if the coach you are interviewing is the one for the job. A professional coach will provide an...their track record? Look for a coach that specializes in scientist coaching. Always ask for references...to the coach about their “style” and make sure this is a style that will help you. A good coach usually...

...explore alternate approaches. Being a “lazy” geneticist, I developed a co-selection approach relying on repair...with unprecedented ease. These new C. elegans approaches rapidly enrich for editing events without the...phenotypes (unc-119) (Dickinson et al., 2013). These approaches allowed effective recovery of knock-ins, but ...several manuscripts published in Genetics detailed approaches in which selection for an editing event that ...and knock-ins at other genomic loci. The first approach – co-CRISPR from Craig Mello’s lab – used inactivation...animals with particular visible phenotypes. These approaches may become even more powerful following a report...Paix et al., 2014). A further development of this approach reported in 2017 uses the direct injection of ...

...thanks to our guest bloggers Joachim Goedhard and Marieke Mastop! Joachim Goedhart is an assistant professor...This post was contributed by guest bloggers Joachim Goedhart and Marieke Mastop from the Section of Molecular...fluorescent probes. You can follow him on twitter: @joachimgoedhart.   Marieke Mastop is a PhD candidate at the...

...single-cell) approach is to interrogate state with qPCR. The re-arrangement of DNA segments in each state of... our paper, “Synthetic recombinase-based state machines in living cells” (1), into a more straight-forward...our engineered devices, recombinase-based state machines for your own experimental needs. For more context...the paper. Why might one be interested in state machine technology? Biological research has produced a ...timing of these factors. Recombinase-based state machines (RSMs), which take on a particular DNA-sequence... prescribed region of DNA termed the “register”. Each chemical input to an RSM drives expression of a ... set of possible downstream states that can be achieved by expression of other recombinases. Multiple ...

...conducted by European Outreach Scientist Benoit Giquel. Ben recently spoke with Joachim Goεdhart, a professor...the interview with Joachim Goedhart Podcast breakdown Intro Chapter 1 - How Joachim ended up at the University...art. Outro Many thanks to our guest, Joachim Goεdhart! Joachim Goedhart is an assistant professor at...University of Amsterdam Chapter 2 - Joachim’s Academic and career goals. Career advice for people going... microscopy. Chpater 4 - Research projects in Joachim’s lab. New microscopy probes and more. Chapter 5...fluorescent probes. You can follow him on twitter: @joachimgoedhart.   Special thanks to our sound engineer/producer...Fluorescent Proteins featured topic page Read Joachim's Series on Choosing the B(right)est Fluorescent...

...replication of RNA in the absence of evolved enzymatic machinery (i.e. RNA replication with no proteins). The chemistry...

...new possibilities for GFP-labeled lines, and they each have their own advantages. T-DOGGs can be made drug-inducible...

...store multiple types of tube for each plasmid we have at Addgene. Each sample at Addgene has four layers...image below). Each plate has numbers and letters to give coordinates, from A1 through H12. Each plate is also...media with antibiotics at each stage. The barcodes and LIMS help us track each sample, but we also have...? Different shelves? Different racks on each shelf? Give each container a specific location to know exactly... Addgene’s lab, a series of events is triggered. Each step is tracked through barcoded tubes that are ...Software    The plasmid's journey begins First, each package is opened and the contents are carefully...have been transported across oceans and continents. Each deposit may consist of anywhere from one to hundreds...

... Other variants of Spinach include Baby Spinach, a miniaturized version of Spinach that retains similar... Hammond’s group created Spinach2 using site-directed mutagenesis of Spinach. In doing so, they identified...was consequently brighter than Spinach. The initial studies using Spinach2 showed its ability to track RNA...imaging. Bunch of baby spinach, or BoBS, contains tandem repeats of Baby Spinach. It was developed in a...twice as bright as Spinach2 when bound to DFHBI. It’s also smaller than any form of Spinach - only 49 nucleotides...Addgene’s collection. Green fluorescent aptamers Spinach and Broccoli and their derivatives are aptamers... and non toxic to cells. Because it rapidly photobleaches, DFHBI can only be used for qualitative, but...

...: 3325793 Schumacher MA (2012) Bacterial plasmid partition machinery: a minimalist approach to survival...control plasmid replication of each plasmid using unique replication machinery. However, when plasmids have...NTPase (Schumacher, 2012).  During cell division, CBPs bind to the centromere-like region on each plasmid... partitioning each type of plasmid into a separate daughter cells (Figure 2) (Ebersbach et al., 2005)....origin of replication and the replication control machinery (Need a refresher on the Origin of Replication...they compete for the same replication control machinery within the cell. Most plasmids encode a negative...through inhibiting translation of replication machinery proteins, or directly inhibit replication by binding...

.... This is the direct approach to immunoimaging assays. But if the direct approach isn’t giving you enough...indirectly, with each protein of interest.    Figure 1: The direct approach (left) versus the...almost always use the indirect approach, while flow cytometry’s standard approach is the direct method. ELISAs...immunocytochemistry may all use either the direct approach or indirect approach, depending on the requirements of the...this is much easier said than done! Each technique, and sometimes each individual assay, will need to be...interest, it may be time to look at the indirect approach - which means it’s time to talk about secondary...binds to the primary antibody. In the indirect approach, the signaling molecule is conjugated to the secondary...

...a fluorophore will bleach. This is, however, not the full story. The photobleaching rate does not depend... 2-fold does not reduce photobleaching by half. How exactly the photobleaching rate depends on power is...experiments. As the photobleaching rate is not linearly related to power, the conclusions reached at high power...thanks to our guest bloggers Joachim Goedhart and Marieke Mastop! Joachim Goedhart is an assistant professor...This post was contributed by guest bloggers Joachim Goedhart and Marieke Mastop from the Section of Molecular...previous post in this series described a practical approach to selecting a bright fluorescent protein. In ...to select a photostable fluorescent protein. Photobleaching is the irreversible destruction of a fluorophore...

...-charge from the ABRC Outreach website. Seeds, which are free for K-12 teachers and available for a nominal...support. Growing capacity - ABRC outreach today Over the years, the outreach efforts of ABRC have grown. A...In 2017 alone, ABRC staff reached more than 4,000 people through our outreach efforts. Our education partnerships... you a biology teacher or professor? Whether you are looking to develop a new teaching resource or you... guest blogger Courtney Price, the Education & Outreach Specialist for the Arabidopsis Biological Resource...samples to researchers and educators in 60 countries each year. Introducing Arabidopsis Arabidopsis is an ...interested in studying animals than plants, and biology teachers’ preference for using animal systems to demonstrate...

...when our middle school’s 8th grade science teachers reached out to their students’ STEM-oriented parents...presentation five times to groups of about 50 students each, and each group went on its own wonderful tangents. Some...about how I could combine all of these roles by reaching out to my school district not just as a dad, but...presentations. If you’re a scientist thinking about reaching out to your community’s schools, here are a few...technology into the school curriculum. Our Parent Teacher Association (PTA) has been instrumental in supplementing... means to request funding for events, consider reaching out to it. PTAs have more flexibility than the...math tables!  Be realistic about what you can teach  5-10 year olds in a single evening. Grades K-5 ...

...students and teachers can investigate together. Teachers decide which BioBuilder lessons to teach, and how.... A summer teacher professional development program that trains nearly 100 teachers each year, with workshops...needs of students and teachers. It’s possible to join a half-day workshop for teachers, to schedule a three-day...synthetic biology into the hands of students and teachers in the U.S. and beyond. “I want to learn more ... a question more and more students are asking. Teachers are also asking, “How do I engage my students .... Built primarily from her 20 years of college teaching experience, Kuldell sought to create a program...Educational Foundation in partnership with high school teachers to boost interest and understanding around the...