What is Polymerase Chain Reaction (PCR)
Type
Protocol
...master mix to add to each PCR tube. Secure the tops to the PCR tubes, gently tap each tube to bring all ...DMSO to each reaction. The ideal setup for this troubleshooting step is to do one reaction with each, and...stable within this temperature range to anneal to each of the single stranded DNA template strands. The...synthesized strands. Materials List Reagents for each 50µL PCR : Thin-walled PCR tube Ice Bucket 2 μL ... 10X Taq buffer with MgCl 2 1 μl dNTP mix (10 mM each nt) 2.5 μL Forward Primer (10 μM stock) 2.5 μL Reverse...2 O 0.2 μL Taq DNA Polymerase (5 units/μL) PCR Machine Agarose Gel Procedure Primer Design and PCR Design...