Differentially Optimized Cell-Free Buffer Enables Robust Expression from Unprotected Linear DNA in Exonuclease-Deficient Extracts.
Batista AC, Levrier A, Soudier P, Voyvodic PL, Achmedov T, Reif-Trauttmansdorff T, DeVisch A, Cohen-Gonsaud M, Faulon JL, Beisel CL, Bonnet J, Kushwaha M
ACS Synth Biol. 2022 Jan 16. doi: 10.1021/acssynbio.1c00448. PubMed Article
Batista AC, Levrier A, Soudier P, Voyvodic PL, Achmedov T, Reif-Trauttmansdorff T, DeVisch A, Cohen-Gonsaud M, Faulon JL, Beisel CL, Bonnet J, Kushwaha M
ACS Synth Biol. 2022 Jan 16. doi: 10.1021/acssynbio.1c00448. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 176580 | BL21 ΔrecB | E. coli BL21 strain (with recB genomic locus deleted) for making cell-free extracts for Linear DNA expression. |
| 176581 | BL21 ΔrecBCD | E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. |
| 176582 | BL21 Rosetta2 ΔrecB | E. coli BL21 strain (with recB genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 plasmid. |
| 176583 | BL21 Rosetta2 ΔrecBCD | E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 plasmid. |
| 176584 | BL21 Rosetta2 GamS | E. coli BL21 strain for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids. |
| 176585 | BL21 Rosetta2 ΔrecB GamS | E. coli BL21 strain (with recB genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids. |
| 176586 | BL21 Rosetta2 ΔrecBCD GamS | E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids. |