We narrowed to 16,573 results for: omp

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pDONR207 SARS-CoV-2 ORF7A_nostop

Plasmid

#149324

Purpose

Gateway-compatible Entry vector, with insert of ORF7A gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF7A (ORF7a SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pEBTet-SNAP-ALMS1

Plasmid

#136828

Purpose

Mammalian expression of the centrosomal protein ALMS1 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-ALMS1 (ALMS1 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ239

Plasmid

#108859

Purpose

FnCpf1 Gateway entry plasmid

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

Insert

FnCpf1

Use

CRISPR; Gateway compatible cpf1 entry clone

Tags

NLS

Expression

Plant

Mutation

Cpf1 is rice codon optimized

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pJZC32

Plasmid

#62327

Purpose

sgRNA (no RNA aptamer addition) with MCP-VP64 effector for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA
MCP-VP64

Use

Lentiviral

Tags

VP64

Expression

Mammalian

Mutation

Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

Promoter

CMV and U6

Available Since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3-V5-PTPN14-PPxY

Plasmid

#61006

Purpose

Expresses human PTPN14 (PPxY domain mutant) in mammalian cells, N-terminus V5 tag

Depositor

Jianmin Zhang

Article

Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

Insert

PTPN14 PPxY mutation (PTPN14 Human)

Tags

V5 tag

Expression

Mammalian

Mutation

PPxY mutation

Promoter

CMV

Available Since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only
pSplit-ON-hDib1

Plasmid

#58787

Purpose

Express hDib1-LucN fusion protein in mammalian cells

Depositor

Hiroyoshi Ariga

Article

Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

Insert

hDib1 (TXNL4A Human)

Tags

Flag and Luciferase (1-398aa)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-myc-AP1S3

Plasmid

#58292

Purpose

mammalian expression of AP1S3

Depositor

Francesca Capon

Article

Setta-Kaffetzi et al Am J Hum Genet. 2014 May 1;94(5):790-7.

Insert

AP1S3 (AP1S3 Human)

Tags

6xHis and myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2014

Availability

Academic Institutions and Nonprofits only
MSCV-myrAkt

Plasmid

#49267

Purpose

retroviral expression of Bovine Akt with the NH2-terminal myristylation-palmitylation signal sequence

Depositor

Lawrence Kane, Arthur Weiss

Article

Kane et al Nat Immunol. 2001 Jan;2(1):37-44.

Insert

Akt (AKT1 Bovine)

Use

Retroviral

Tags

IRES-EGFP and myr

Expression

Mammalian

Mutation

N2D, D149E and A478G compared to AAW71957.1

Available Since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only
pET12a-AL-09 FL

Plasmid

#47081

Depositor

Marina Ramirez-Alvarado

Article

McLaughlin et al Protein Sci. 2006 Jul;15(7):1710-22. Epu

Insert

AL-09 (IGKV3-20 Human)

Tags

ompT

Expression

Bacterial

Promoter

T7

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO DNAJB5

Plasmid

#19473

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJB5 (DNAJB5 Human)

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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M10.2-IRES-tauVenus-ACNF Targeting Vector

Plasmid

#15858

Depositor

Peter Mombaerts

Article

Ishii et al J Neurosci. 2008 Mar 5. 28(10):2332-41.

Insert

M10.2

Expression

Bacterial

Available Since

May 6, 2008

Availability

Academic Institutions and Nonprofits only
AP1muA-mStayGold-polyA-Hygro HR

Plasmid

#229679

Purpose

Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with monomeric StayGold. Contains a hygromycin resistance cassette for selection of edited cells

Depositor

Francesca Bottanelli

Article

Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

Insert

genomic sequence AP1M1 locus (AP1M1 Human)

Use

CRISPR

Tags

monomeric StayGold

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-Cep131

Plasmid

#136826

Purpose

Mammalian expression of the centrosomal protein Cep131 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep131 (CEP131 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-INTS3-FL

Plasmid

#128415

Purpose

Express INTS3 full-length in E. coli with a GST tag (N-terminal)

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

Insert

INTS3 (INTS3 Human)

Tags

GST

Expression

Bacterial

Mutation

full-length

Promoter

tac promoter

Available Since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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5HT6-YFP-ThymosinBeta4(KK18,19EE)

Plasmid

#96807

Purpose

non-actin-binding control for 5HT6-YFP-ThymosinBeta4(WT)

Depositor

Takanari Inoue

Article

Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

Insert

5HT6-YFP-ThymosinBeta4 (Tmsb4x Mouse)

Tags

YFP

Expression

Mammalian

Mutation

KK18,19EE prevents G-actin binding

Available Since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pILGFPI7

Plasmid

#83555

Purpose

Used to evaluate the expression output of CUP1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

CUP1 promoter-yEGFP

Expression

Yeast

Promoter

CUP1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-LplA(AAG)

Plasmid

#61307

Purpose

Contains FLAG epitope for immunofluorescence detection of resorufin ligase for mammalian cell expression

Depositor

Alice Ting

Article

Liu et al Proc Natl Acad Sci U S A. 2014 Oct 28;11

Insert

E. coli lipoic acid ligase

Tags

6xHis, Flag, T7 tag, and Xpress epitope

Expression

Mammalian

Mutation

E20A, F147A, H149G

Promoter

CMV

Available Since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

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GST-PRDM16 1039-1176

Plasmid

#53351

Purpose

bacterial expression of mouse PRDM16 fragment

Depositor

Shingo Kajimura, Bruce Spiegelman

Article

Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

Insert

PRDM16 (Prdm16 Mouse)

Tags

GST

Expression

Bacterial

Mutation

aa 1039-1176

Promoter

tac

Available Since

June 2, 2014

Availability

Academic Institutions and Nonprofits only
GST-PRDM16 681-880

Plasmid

#53349

Purpose

bacterial expression of mouse PRDM16 fragment

Depositor

Shingo Kajimura, Bruce Spiegelman

Article

Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

Insert

PRDM16 (Prdm16 Mouse)

Tags

GST

Expression

Bacterial

Mutation

aa 681-880

Promoter

tac

Available Since

June 2, 2014

Availability

Academic Institutions and Nonprofits only
GST-PRDM16 455-680

Plasmid

#53348

Purpose

bacterial expression of mouse PRDM16 fragment

Depositor

Shingo Kajimura, Bruce Spiegelman

Article

Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

Insert

PRDM16 (Prdm16 Mouse)

Tags

GST

Expression

Bacterial

Mutation

aa 455-680

Promoter

tac

Available Since

June 2, 2014

Availability

Academic Institutions and Nonprofits only