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Addgene

We narrowed to 4,028 results for: LYC;

Showing: 3481 - 3500 of 4028 results
  1. B-HypaR-SpCas9

    Plasmid
    #126764
    Purpose
    Expression plasmid for human codon-opt. increased fidelity Blackjack-Hypa2SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than Hypa2SpCas9.
    Depositor
    Insert
    B-Hypa2SpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    R661A; N692A; M694A; Q695A; H698A; amino acids 10…
    Promoter
    CBh
    Available since
    Sept. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
  2. GB1-PRD(703-868, P801G, T805C, C813S)-Strep

    Plasmid
    #169749
    Purpose
    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G, T805C, C813S)-Strep.
    Depositor
    Insert
    GB1-His6-TEV-ALIX(703-868, P801G, G808C, C813S)-Strep (PDCD6IP Human)
    Use
    Tags
    GB1-His6-TEV and Strep tag (WSHPQFEK)
    Expression
    Bacterial
    Mutation
    Proline 801 to Glycine, Threonine 805 to Cysteine…
    Promoter
    Available since
    May 26, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. GB1-PRD(703-868, S712C, P801G, C813S)-Strep

    Plasmid
    #164445
    Purpose
    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, S712C, P801G, C813S)-Strep.
    Depositor
    Insert
    GB1-His6-TEV-ALIX(703-868, S712C, P801G, C813S)-Strep (PDCD6IP Human)
    Use
    Tags
    GB1-His6-TEV and Strep tag (WSHPQFEK)
    Expression
    Bacterial
    Mutation
    Serine 712 to Cysteine, Proline 801 to Glycine, C…
    Promoter
    Available since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. GB1-PRD(703-868, A756C, P801G, C813S)-Strep

    Plasmid
    #164446
    Purpose
    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, A756C, P801G, C813S)-Strep.
    Depositor
    Insert
    GB1-His6-TEV-ALIX(703-868, A756C, P801G, C813S)-Strep (PDCD6IP Human)
    Use
    Tags
    GB1-His6-TEV and Strep tag (WSHPQFEK)
    Expression
    Bacterial
    Mutation
    Alanine 756 to Cysteine, Proline 801 to Glycine, …
    Promoter
    Available since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. GB1-PRD(703-868, P801G, C813S, S863C)-Strep

    Plasmid
    #164447
    Purpose
    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G, C813S, S863C)-Strep.
    Depositor
    Insert
    GB1-His6-TEV-ALIX(703-868, P801G, C813S, S863C)-Strep (PDCD6IP Human)
    Use
    Tags
    GB1-His6-TEV and Strep tag (WSHPQFEK)
    Expression
    Bacterial
    Mutation
    Proline 801 to Glycine, Cysteine 813 to Serine, S…
    Promoter
    Available since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. pIFU002

    Plasmid
    #174299
    Purpose
    Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the N501Y mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombination
    Depositor
    Insert
    Spike RBD N343Q, N501Y (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    HA and cMyc
    Expression
    Yeast
    Mutation
    N343Q, N501Y
    Promoter
    Available since
    Sept. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
  7. pIFU001

    Plasmid
    #174298
    Purpose
    Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the E484K mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombination
    Depositor
    Insert
    Spike RBD N343Q, E484K (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    HA and cMyc
    Expression
    Yeast
    Mutation
    N343Q, E484K
    Promoter
    Available since
    Sept. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
  8. pVD1 M3_3-pTRNA-scf 2.1 (GB2075)

    Plasmid
    #160560
    Purpose
    tRNA and scaffold 2.1scRNA aptamer Ms2 for the assembly of GBoligomers for the first position (positon [M3_3]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter
    Depositor
    Insert
    M3_3-pTRNA-scf 2.1
    Use
    CRISPR
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  9. pVD1 M1_3-pTRNA-scf 2.1 (GB2073)

    Plasmid
    #160558
    Purpose
    tRNA and scaffold 2.1scRNA aptamer Ms2 for the assembly of GBoligomers for the first position (positon [M1_3]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.
    Depositor
    Insert
    M1_3-pTRNA-scf 2.1
    Use
    CRISPR
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  10. pVD1 M2_3-pTRNA-scf 2.1 (GB2074)

    Plasmid
    #160559
    Purpose
    tRNA and scaffold 2.1scRNA aptamer Ms2 for the assembly of GBoligomers for the first position (positon [M2_3]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter
    Depositor
    Insert
    M2_3-pTRNA-scf 2.1
    Use
    CRISPR
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Feb. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
  11. pPICZ-His6-K2P4.1A-mCherry-StreptagII

    Plasmid
    #158743
    Purpose
    Expresses K2P4.1 isoform A with TEV protease cleavable N- and C-terminal affinity tags.
    Depositor
    Insert
    K2P4.1-A (KCNK4 Human)
    Use
    Tags
    His6 and mCherry with Streptag II
    Expression
    Yeast
    Mutation
    Residues 1-264 with N-linked glycosylation sites …
    Promoter
    AOX1
    Available since
    Sept. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 3481 - 3500 of 4028 results