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pcDNA5-FRT-TO-FH-Nsp14

Plasmid

#157718

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp14 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Publication

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp14 (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Available since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-FH-Nsp2

Plasmid

#157683

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp2 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Publication

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp2 (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Available since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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RRL.sin.cPPT.SFFV/Ace2.WPRE (MT136)

Plasmid

#145842

Purpose

Lentiviral vector genome to ectopically express human Ace2

Depositor

Caroline Goujon

Publication

Rebendenne et al J Virol. 2021 Jan 29. pii: JVI.02415-20.

Insert

human Ace2 (ACE2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pAAV-hSyn-mCherry.3xFLAG.NOS1AP-WPRE

Plasmid

#127864

Purpose

pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Publication

Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

Insert

Nos1ap (Nos1ap Mouse)

Use

AAV

Tags

3xFLAG and mCherry

Promoter

hSyn

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCellFree_G03 DDX5

Plasmid

#67111

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Publication

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

DDX5 (DDX5 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
mTFP1-Lysosomes-20

Plasmid

#55495

Purpose

Localization: Lysosome Membrane, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Publication

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

LAMP1

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pQTEV-MPP6

Plasmid

#34806

Depositor

Konrad Buessow

Publication

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

membrane protein, palmitoylated 6 (PALS2 Human)

Tags

His and TEV

Expression

Bacterial

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-RELΔRID

Plasmid

#27265

Depositor

Thomas Gilmore

Publication

Leeman et al Oncogene. 2008 Dec 4. 27(53):6770-81.

Insert

REL (REL Human)

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of aa 323-422

Available since

Feb. 13, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pIOM15

Plasmid

#20869

Depositor

Carmen Beuzón

Publication

Ortiz-Martin et al J Microbiol Methods. 2006 Dec . 67(3):39

Insert

aphA::aadA

Expression

Bacterial

Available since

July 15, 2009

Availability

Academic Institutions and Nonprofits only
pC1-dmito-oROS-HT

Plasmid

#216418

Purpose

Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to mitochondrial matrix.

Depositor

Andre Berndt

Publication

Lee et al bioRxiv 2024.02.06.579232

Insert

dmito-oROS-HT

Expression

Mammalian

Promoter

CMV

Available since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-V3-CD20-Puro

Plasmid

#209757

Purpose

Lentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1. Specifically, the variant 3 mRNA isoform.

Depositor

Andrei Thomas-Tikhonenko

Publication

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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GFP SMG1 WT

Plasmid

#199571

Purpose

Expresses WT SMG1 fused to GFP

Depositor

Michael Kastan

Publication

McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

Insert

SMG1 WT (SMG1 Human)

Tags

GFP fusion protein

Expression

Mammalian

Promoter

CMV

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pRM15

Plasmid

#163694

Purpose

hsp70 promoter inducible expression of DHB:mScarlet-P2A-H2B:miRFP670

Depositor

David Matus

Publication

Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1

Insert

DHB:mScarlet-P2A-H2B:miRFP670 (HELB, H2BC11 Human)

Use

Zebrafish expression

Tags

mScarlet and miRFP670

Promoter

HSP70

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_SNORD27h

Plasmid

#73066

Purpose

expression clone for human SNORD27 (C/D box snoRNA U27)

Depositor

Stefan Stamm

Publication

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

SNORD27 (SNORD27 Human)

Tags

no tag

Expression

Mammalian

Mutation

expression cassette for SNORD27

Promoter

CMV

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pRSET-pr-mEos3.1 (mEosFP3.1-A69T)

Plasmid

#99221

Purpose

Bacterial expression of the primed conversion capable protein pr-mEos3.1 (mEos3.1-A69T).

Depositor

Periklis Pantazis

Publication

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEos3.1

Expression

Bacterial

Mutation

A69T for primed conversion applications

Promoter

T7

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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mTFP1-Tubulin-6

Plasmid

#55512

Purpose

Localization: Microtubules, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Publication

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Tubulin (TUBA1B Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pSARSF505

Plasmid

#46832

Depositor

Hideo Iwai

Publication

Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

Insert

GFPN-NpuDnaE_intein-GFP_C

Tags

GFP_C and H6-GFP_N

Expression

Bacterial

Mutation

stemmer GFP with superfolder mutations S30R and Y…

Promoter

T7

Available since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pAL1

Plasmid

#197285

Purpose

Shuttle plasmid that replicates in E. coli, S. cerevisiae, and A. laidlawii. Contains the OriC from A. laidlawii 8195

Depositor

Bogumil Karas

Publication

Nucifora et al ACS Synth Biol. 2024 Jan 19;13(1):45-53.

Insert

Origin of replication of A. laidlawii 8195: whole dnaA gene, plus upstream and downstream intergenic regions

Use

Synthetic Biology; Bacterial and yeast cloning

Available since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

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Tip alpha (28-192)

Plasmid

#23158

Depositor

SeWon Suh

Publication

Jang et al J Mol Biol. 2009 Sep 11. 392(1):191-7.

Insert

TNF-α-inducing protein

Tags

His

Expression

Bacterial

Mutation

deleted amino acids 1-27

Available since

April 16, 2010

Availability

Academic Institutions and Nonprofits only
LV2btCRISPRko.v1

Pooled library

#213927

Purpose

Uses lentivirus to deliver the library into hard-to-transduce cells

Depositor

Wenfang Spring Tan

Publication

Tan et al PLoS Pathog. 2023 Dec 6;19(12):e1011822.

Expression

Mammalian

Use

CRISPR and Lentiviral

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only