We narrowed to 4,523 results for: ARA-2

1. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CAMK1D TSS-guide1

   Plasmid

   #118177

   Purpose

   CRISPR-mediated repression of CAMK1D. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   hPGK and U6

   Available Since

   April 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-TO-oNGN2

   Plasmid

   #198397

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Inserts

   NEUROG2 (phospho mutant, codon optimized) (NEUROG2 Human)
   puroR-T2A-mycNLS-mTagBFP2
   rtTA3G
   

   Tags

   T2A-mycNLS-mTagBFP2

   Expression

   Mammalian

   Mutation

   S24A, S193A, S207A, S209A, S219A, S232A, S239A, S…

   Promoter

   CAG, EF1a, and TRE3G

   Available Since

   April 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-TO-NGN2-EMX1 BSD-mApple

   Plasmid

   #182311

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 and EMX1 expression, bsd selection, mApple

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Inserts

   hNGN2-P2A-EMX1 (NEUROG2, EMX1 Human)
   bsd-T2A-mycNLS-mApple
   

   Tags

   T2A-mycNLS-mApple

   Expression

   Mammalian

   Promoter

   EF1a and TRE3G

   Available Since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-tetO-NGN2

   Plasmid

   #197090

   Purpose

   PiggyBac plasmid with tet-inducible expression of transcription factors for cortical neuron differentiation

   Depositor

   Brian Wainger

   Article

   Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

   Insert

   NGN2 (NEUROG2 Human)
   

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUDP044

   Plasmid

   #101168

   Purpose

   pUDP004 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes SeATF1 and SeATF2 and Spcas9D147Y P411T in S. pastorianus (HH-gRNASeATF1-HDV-linker-HH-gRNASeATF2-HDV)

   Depositor

   Jean-Marc Daran

   Article

   de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

   Insert

   polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting SeATF1 and 2 in S. pastorianus
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   ScTDH3

   Available Since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKM 1995

   Plasmid

   #217826

   Purpose

   Expresses 1554 bp fragment of HY4 gene in A. thaliana. This HY4 fragment shows high homology to the phr genes in prokaryotes.

   Depositor

   Aziz Sancar

   Article

   Malhotra et al Biochemistry. 1995 May 23;34(20):6892-9.

   Insert

   HY4 (CRY1 Mustard Weed)
   

   Tags

   Maltose-binding protein

   Expression

   Bacterial

   Available Since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pC-0_v6

   Plasmid

   #124426

   Purpose

   Full MCS, lacks araC & P(BAD) (step 2)

   Depositor

   Yolanda Schaerli

   Article

   Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   June 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pC-0_v4

   Plasmid

   #124424

   Purpose

   Full MCS, lacks araC & P(BAD) (step 2)

   Depositor

   Yolanda Schaerli

   Article

   Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   June 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJRK246

   Plasmid

   #173741

   Purpose

   donor plasmid for SapTrap assembly

   Depositor

   Mike Boxem

   Article

   Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

   Insert

   wrt-2 promoter
   

   Use

   Other

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJRK143

    Plasmid

    #173737

    Purpose

    donor plasmid for SapTrap assembly

    Depositor

    Mike Boxem

    Article

    Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

    Insert

    elt-2 promoter
    

    Use

    Other

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAC2-dual-dCas9VP48-sgExpression

    Plasmid

    #48236

    Purpose

    Dual expression construct expressing both dCas9VP48 and sgRNA from separate promoters

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9VP48
    

    Use

    CRISPR

    Tags

    HA-tag and VP48

    Expression

    Mammalian

    Mutation

    D10A H840A (catalytically inactive)

    Available Since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAC5-dual-dCas9VP48-sgTetO

    Plasmid

    #48237

    Purpose

    Dual expression construct expressing both dCas9VP48 and sgTetO from separate promoters

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9VP48 and sgTetO
    

    Use

    CRISPR

    Tags

    HA-tag and VP48

    Expression

    Mammalian

    Mutation

    D10A H840A (catalytically inactive)

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. Yiplac211 proGAL1-FRB-RGG-GFP-RGG-RGG

    Plasmid

    #176400

    Purpose

    Expresses GFP tagged triple RGG scaffold with N-terminal FRB. Expression induced in media containing galactose.

    Depositor

    Matthew Good

    Article

    Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

    Insert

    FRB-RGG-GFP-RGG-RGG
    

    Tags

    FRB and GFP

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSBK.013

    Plasmid

    #187213

    Purpose

    Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 5 [ACG-CGT]), and Cas1 + Cas2

    Depositor

    Seth Shipman

    Article

    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

    Inserts

    Retron Eco1 ncRNA v35 (long a1/a2, barcode 5 [ACG-CGT])
    Cas1 + Cas2
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7/Lac

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSBK.014

    Plasmid

    #187215

    Purpose

    Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 6 [CAG-CTG]), and Cas1 + Cas2

    Depositor

    Seth Shipman

    Article

    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

    Inserts

    Retron Eco1 ncRNA v35 (long a1/a2, barcode 6 [CAG-CTG])
    Cas1 + Cas2
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7/Lac

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSBK.009

    Plasmid

    #187209

    Purpose

    Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 1 [CCT-AGG]), and Cas1 + Cas2

    Depositor

    Seth Shipman

    Article

    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

    Inserts

    Retron Eco1 ncRNA v35 (long a1/a2, barcode 1 [CCT-AGG])
    Cas1 + Cas2
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7/Lac

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUBC(k)-AT4G33650-mKOk

    Plasmid

    #224846

    Purpose

    Plasmid for expression of AT4G33650.1 coding sequence tagged with mKOk in plants

    Depositor

    Rebecca Roston

    Article

    LaBrant et al bioRxiv 2025.06.20.660776; doi: https://

    Insert

    AT4G33650.1 (DRP3A Mustard Weed)
    

    Expression

    Plant

    Available Since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. M2C5

    Plasmid

    #188606

    Purpose

    Minimal Conjugative Plasmid 2

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Diatom expression

    Expression

    Bacterial and Yeast

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSBK.011

    Plasmid

    #187211

    Purpose

    Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 3 [CTG-CAG]), and Cas1 + Cas2

    Depositor

    Seth Shipman

    Article

    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

    Inserts

    Retron Eco1 ncRNA v35 (long a1/a2, barcode 3 [CTG-CAG])
    Cas1 + Cas2
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7/Lac

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSBK.012

    Plasmid

    #187212

    Purpose

    Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 4 [GTG-CAC]), and Cas1 + Cas2

    Depositor

    Seth Shipman

    Article

    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

    Inserts

    Retron Eco1 ncRNA v35 (long a1/a2, barcode 4 [GTG-CAC])
    Cas1 + Cas2
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7/Lac

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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