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pUPD3_PARTPRM_J23103

Plasmid

#243933

Purpose

GoldenBraid2.0 Constitutive promoter, lacking ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

J23103

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_Pem7

Plasmid

#243908

Purpose

GoldenBraid2.0 Strong, constitutive promoter, including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

Pem7

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_Pem7

Plasmid

#243919

Purpose

GoldenBraid2.0 Strong, constitutive promoter, not including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

Pem7

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pBSKa1_Stuffer1

Plasmid

#243844

Purpose

GoldenBraid2.0 Full transcription unit stuffer in an alpha-level cloning vector.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

Stuffer1

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pBSKa2_Stuffer2

Plasmid

#243847

Purpose

GoldenBraid2.0 Full transcription unit stuffer in an alpha-level cloning vector.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

Stuffer2

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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ISA861: Melezitose pathway

Plasmid

#241971

Purpose

Melezitose pathway phagemid. Contains p15A, CmR, pac site, AraC, C1, and pBAD.

Depositor

Nathan Crook

Article

Al'Abri et al Nucleic Acids Res. 2022 Jun 10;50(10):e5

Insert

melezitose pathway

Use

Synthetic Biology; Phagemid

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pYO503

Plasmid

#235743

Purpose

Protein expression of KmTor by integrating ZZ-KmTOR into the K. marxianus genome

Depositor

Roger Williams

Article

Baretic et al Nat Commun. 2016 Apr 13;7:11016. doi: 10

Insert

KmTOR

Tags

ZZ-3xTEV

Expression

Yeast

Mutation

aa 4-4609

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYO524

Plasmid

#235744

Purpose

Protein expression of KmLst8 by integrating KmLST8 into the K. marxianus genome

Depositor

Roger Williams

Article

Baretic et al Nat Commun. 2016 Apr 13;7:11016. doi: 10

Insert

KmLST8

Expression

Yeast

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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MKT071

Plasmid

#231995

Purpose

MicF RNA toehold switch with sfgfp reporter

Depositor

Melissa Takahashi

Article

Diaz et al MicroPubl Biol. 2025 Jan 2;2025:10.17912

Insert

MicF RNA toehold switch

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pKM596-AePHOT-LOV1

Plasmid

#220113

Purpose

The expression of MBP fused to AePHOT-LOV1 in E. coli

Depositor

Yutaka Kodama

Article

Yong et al Photochem Photobiol. 2023 Oct 26. doi: 1

Insert

AePHOT-LOV1

Expression

Bacterial

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pKM596-MpPHOT-LOV1

Plasmid

#220115

Purpose

The expression of MBP fused to MpPHOT-LOV1 in E. coli

Depositor

Yutaka Kodama

Article

Yong et al Photochem Photobiol. 2023 Oct 26. doi: 1

Insert

MpPHOT-LOV1

Expression

Bacterial

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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DVK_pBR322_FG

Plasmid

#217595

Purpose

Destination vector with kanamycin resistance and pBR322 origin of replication. Carries F-G CIDAR MoClo overhangs for level 1 construct cloning.

Depositor

Wei Huang

Article

Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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DVK_pBR322_AF

Plasmid

#217593

Purpose

Destination vector with kanamycin resistance and pBR322 origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.

Depositor

Wei Huang

Article

Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-a(+)-6xHis-mScarlet-I(+5)

Plasmid

#219418

Purpose

Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

Depositor

Allie Obermeyer

Article

Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

Insert

mScarlet-I(+5)

Tags

MGHHHHHGG

Expression

Bacterial

Mutation

D125K, D186K, E216K, E238K

Promoter

T7

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGWT35S-AePHOT-Citrine

Plasmid

#220105

Purpose

Transient expression of AePHOT-Citrine in plant cells

Depositor

Yutaka Kodama

Article

Yong et al Photochem Photobiol. 2023 Oct 26. doi: 1

Insert

AePHOT-Citrine

Expression

Plant

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pKM596-AePHOT-LOV2

Plasmid

#220114

Purpose

The expression of MBP fused to AePHOT-LOV2 in E. coli

Depositor

Yutaka Kodama

Article

Yong et al Photochem Photobiol. 2023 Oct 26. doi: 1

Insert

AePHOT-LOV2

Expression

Bacterial

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pKM596-MpPHOT-LOV2

Plasmid

#220116

Purpose

The expression of MBP fused to MpPHOT-LOV2 in E. coli

Depositor

Yutaka Kodama

Article

Yong et al Photochem Photobiol. 2023 Oct 26. doi: 1

Insert

MpPHOT-LOV2

Expression

Bacterial

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1503

Plasmid

#218610

Purpose

Express Pex30

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex30

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1504

Plasmid

#218611

Purpose

Express Pex31

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex31

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1505

Plasmid

#218612

Purpose

Express Pex35

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex35

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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