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pBS-TarHom-Tol2-kanaR-cryaa-dsRed

Plasmid

#74152

Purpose

BAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker dsRed

Depositor

Holger Knaut

Article

Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

Insert

Tol2-kanaR-cryaa-dsRed

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pBS-TarHom-Tol2-kanaR-cryaa-sfGFP

Plasmid

#74153

Purpose

BAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker sfGFP

Depositor

Holger Knaut

Article

Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

Insert

Tol2-kanaR-cryaa-sfGFP

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pBS-IndHom-Tol2-TetR-cryaa-dsRed

Plasmid

#73204

Purpose

BAC targeting cassette for pIndigoBAC-536 with tetR selection marker and lens-specific transgenesis marker dsRed

Depositor

Holger Knaut

Article

Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

Insert

dsRed

Available Since

March 8, 2016

Availability

Academic Institutions and Nonprofits only

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pBS-TarHom-Tol2-kanaR-cryaa-YFP

Plasmid

#74154

Purpose

BAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker Citrine

Depositor

Holger Knaut

Article

Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

Insert

Tol2-kanaR-cryaa-YFP

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pBS-IndHom-Tol2-FRT-GalK-cryaa-dsRed

Plasmid

#73203

Purpose

BAC targeting cassette for pIndigoBAC-536 with GalK selection marker and lens-specific transgenesis marker dsRed

Depositor

Holger Knaut

Article

Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

Insert

dsRed

Available Since

Sept. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pBS-TarHom-Tol2-FRT-GalK-cryaa-CFP

Plasmid

#73200

Purpose

BAC targeting cassette for pTARBAC2 with GalK selection marker and lens-specific transgenesis marker Cerulean

Depositor

Holger Knaut

Article

Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

Insert

CFP

Available Since

March 8, 2016

Availability

Academic Institutions and Nonprofits only

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pBS-TarHom-Tol2-FRT-GalK-cryaa-YFP

Plasmid

#73198

Purpose

BAC targeting cassette for pTARBAC2 with GalK selection marker and lens-specific transgenesis marker Citrine

Depositor

Holger Knaut

Article

Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

Insert

YFP

Available Since

March 8, 2016

Availability

Academic Institutions and Nonprofits only

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pXCX-sGFAP-LMO-IRES-tdTomato

Plasmid

#178312

Purpose

Express the bacterial lactate 2-monooxygenase (LMO) enzyme in astrocytes

Depositor

Sergey Kasparov

Article

Vaccari Cardoso et al Brain Sci. 2021 Aug 10;11(8). pii: brain

Insert

Lactate 2-monooxygenase

Use

Adenoviral

Tags

tdTomato

Expression

Mammalian

Promoter

sGFAP

Available Since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pXCX-sGFAP-DLDH-IRES-tdTomato

Plasmid

#178311

Purpose

Express the bacterial D-lactate dehydrogenase (DLDH) enzyme in astrocytes

Depositor

Sergey Kasparov

Article

Vaccari Cardoso et al Brain Sci. 2021 Aug 10;11(8). pii: brain

Insert

D-lactate dehydrogenase

Use

Adenoviral

Tags

tdTomato

Expression

Mammalian

Promoter

sGFAP

Available Since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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Villinpromoter-blue-FlpOERT2

Plasmid

#67278

Purpose

4hydroxytamoxifen inducible FlpO recombinase controlled by intestine specific promoter (Villin). FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context

Depositor

Jannik Elverløv-Jakobsen

Article

Callesen et al Mol Oncol. 2017 Nov;11(11):1616-1629. do

Insert

Villin-mTQ2-P2A-FlpO-ERT2

Tags

mTQ2, linked to FlpO through an P2A ribosomal ski…

Expression

Mammalian

Promoter

Villin

Available Since

July 14, 2015

Availability

Academic Institutions and Nonprofits only

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DNA-PKcs PQR>ala

Plasmid

#83319

Purpose

human DNA-PKcs PQR>ala

Depositor

Katheryn Meek

Article

Cui et al Mol Cell Biol. 2005 Dec;25(24):10842-52.

Insert

human DNA-PKcs (PRKDC Human)

Expression

Mammalian

Mutation

5 phosphorylation sites in the PQR cluster substi…

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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DNA-PKcs ABCDE>A

Plasmid

#83318

Purpose

human DNA-PKcs/ABCDE>ala

Depositor

Katheryn Meek

Article

Cui et al Mol Cell Biol. 2005 Dec;25(24):10842-52.

Insert

human DNA-PKcs (PRKDC Human)

Expression

Mammalian

Mutation

6 phosphorylation sites in the ABCDE cluster subs…

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pWZL Blast Snail ER

Plasmid

#18798

Depositor

Bob Weinberg

Article

Mani et al Cell. 2008 May 16. 133(4):704-15.

Insert

snail (SNAI1 Human)

Use

Retroviral

Tags

ER hormone binding domain

Expression

Mammalian

Mutation

G525R mutation in the ER. The presence of the G52…

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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acta2_mCherry_pA_pDestTolpA2

Plasmid

#78685

Purpose

acta2:mCherry transgenesis construct

Depositor

Sarah Childs

Article

Whitesell et al PLoS One. 2014 Mar 3;9(3):e90590. doi: 1

Insert

acta2 promoter (acta2 Zebrafish)

Use

Unspecified

Tags

mCherry

Promoter

acta2

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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acta2_GFP_pA_pDestTolpA2

Plasmid

#78684

Purpose

acta2:GFP transgenesis construct

Depositor

Sarah Childs

Article

Whitesell et al PLoS One. 2014 Mar 3;9(3):e90590. doi: 1

Insert

acta2 promoter (acta2 Zebrafish)

Use

Unspecified

Promoter

acta2

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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Triple ORF LoxP ifgMosaic Donor (LH208)

Plasmid

#99625

Purpose

To clone the 3 ORFs for Cre dependent generation of ifgMosaics

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

N-PhiM

Use

Cre/Lox, Mouse Targeting, and Synthetic Biology

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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LoxP1-His-H2B-Cherry-2A (SO290)

Plasmid

#99616

Purpose

To clone gene of interest downstream of LoxP1-His-H2B-Cherry-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

6xHis-H2B-Cherry

Tags

T2A

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pICE-HA-CtIP-siR-S664A-S679A-S745A

Plasmid

#82031

Purpose

Plasmid for constitutive or doxy-inducible expression of S664A+S679A+S745A human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

Depositor

Sébastien Britton, Patrick Calsou

Article

Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

Insert

CtIP (RBBP8 Human)

Tags

HA

Expression

Mammalian

Mutation

Non phosphorylatable mutations on 3 of the main A…

Promoter

CMV-tet

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pGal-Trans-Myc-HA-GFP11-KDEL

Plasmid

#177724

Purpose

ER protein collagen beta (1-O) galactosyltransferase with a C-terminal Myc tag, an HA tag and the eleventh β-strand of GFP

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

collagen beta(1-O)galactosyltransferase 1 (COLGALT1 Human)

Tags

GFP11, HA, KDEL, and Myc

Expression

Bacterial and Mammalian

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pGEM BirA-2A-Citrine-SV40pA-FRT-Kan-FRT

Plasmid

#89890

Purpose

BAC donor construct containing 3XHA-tagged BirA, a ribosome skipping motif - 2A, Citrine reporter, polyadenyation signal, followed by FRT recombination sites flanking kanamycin selection cassette.

Depositor

Tatjana Sauka-Spengler

Article

Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

Insert

HA-BirA-2A-citrine

Use

Unspecified

Tags

Citrine

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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