We narrowed to 11,282 results for: Vars

1. FLAG HDAC5 WT

   Plasmid

   #32213

   Depositor

   Reuben Shaw

   Article

   Mihaylova et al Cell. 2011 May 13;145(4):607-21.

   Insert

   HDAC5 (HDAC5 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pR1G108CP20X

   Plasmid

   #85086

   Purpose

   Expresses PCNA1 variant (G108C) fused to putidaredoxin reductase and putidaredoxin in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   The G108C variant of PCNA1 fused to putidaredoxin reductase and putidaredoxin
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   G108C in PCNA1

   Promoter

   T7 promoter

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLQ1371-sgGAL4

   Plasmid

   #121514

   Purpose

   sgGAL4 control. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

   Depositor

   Stanley Qi, Ovijit Chaudhuri

   Article

   Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

   Insert

   sgGAL4
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available since

   April 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. gfp-Arkadia/RNF111

   Plasmid

   #112228

   Purpose

   Expresses GFP-tagged Arkadia in mammalian cells

   Depositor

   Vasso Episkopou

   Article

   Mavrakis et al PLoS Biol. 2007 Mar;5(3):e67. doi: 10.13

   Insert

   (Arkadia)Rnf111 (Rnf111 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMDJ17 - pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]

   Plasmid

   #159803

   Purpose

   Expression construct with ubiquitous expression to test fluorescence level on microscope

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-OSH2-2xPH

   Plasmid

   #161987

   Purpose

   Mammalian expression vector, contains two tandem repeats of OSH2 PH domain (PI(4)P biosensor). Fused to eGFP

   Depositor

   Tamas Balla, David Bryant

   Article

   Balla et al Mol Biol Cell. 2008 Feb;19(2):711-21. do

   Insert

   OSH2 (OSH2 Budding Yeast)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBac1-Ls-AChBP

   Plasmid

   #63126

   Purpose

   Expression of Lymnaea stagnalis AChBP

   Depositor

   Titia Sixma

   Article

   Celie et al Nat Struct Mol Biol. 2005 Jul;12(7):582-

   Insert

   AChBP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. LENC

   Plasmid

   #111163

   Purpose

   miR-E (miR-30 variant)-based RNAi with mCherry reporter

   Depositor

   Johannes Zuber

   Article

   Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

   Insert

   miR-E (miR-30 variant)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRK5-hGRK4

   Plasmid

   #32690

   Depositor

   Robert Lefkowitz

   Article

   Premont et al J Biol Chem. 1996 Mar 15;271(11):6403-10

   Insert

   GRK4 (GRK4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pAB078d8

    Plasmid

    #79206

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1+2) luxAB; Ppro1 434cI-SH2ABL1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacZ-opt (OR1+2); Ppro1

    Available since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pV1326

    Plasmid

    #111435

    Purpose

    S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDUP1

    Plasmid

    #223516

    Purpose

    inactive cogfp under control of Ptet, cogfp under control of Ptac

    Depositor

    Yolanda Schaerli

    Article

    Ljiljana et al eLife13:RP97216

    Inserts

    CoGFP
    CoGFP inactivated
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    anti-dimerization mutations S129G, 22 C154S, D156…

    Promoter

    Ptac, Ptac and Ptet, and Ptet

    Available since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA5/FRT/TO_H2B_HaloTag9_T2A_EGFP

    Plasmid

    #169332

    Purpose

    Nuclear expression of HaloTag9 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag9 (H2BC21 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDB180

    Plasmid

    #80677

    Purpose

    for secretion of N-terminal OsmY, deca-histidine, SUMO tagged protein

    Depositor

    David Baker

    Article

    Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

    Type

    Empty backbone

    Use

    Tags

    OsmY-10His-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAB076i5

    Plasmid

    #79199

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1+2+3) gIII, luxAB; Ppro1 434cI-SH2ABL1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacZ-opt (OR1+2+3); Ppro1

    Available since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDV02_linear_entry_TagBFP-2A-MCS-2A-mNeonGreen

    Plasmid

    #200401

    Purpose

    Entry vector for open-loop ADAR-based RNA sensors. Expresses TagBFP constitutively. Expresses mNeonGreen upon target detection

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    Open-loop DART VADAR sensor entry cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCFJ2440 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)

    Plasmid

    #159849

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDest-303

    Plasmid

    #159678

    Purpose

    Gateway destination vector for mammalian protein expression

    Depositor

    Dominic Esposito

    Article

    Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAB078d3

    Plasmid

    #79203

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1) luxAB; Ppro1 434cI-SH2ABL1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacZ-opt (OR1); Ppro1

    Available since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. ECFP HO

    Plasmid

    #207413

    Purpose

    Mammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).

    Depositor

    Karlene Cimprich

    Article

    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

    Insert

    ECFP
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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