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anti-FLAG-CAR (human, optimised, tonic-signal-free)

Plasmid

#247778

Purpose

Anti-FLAG scFv with Myc-tag, human CD8α-truncated hinge, and human CD28-CD3z intracellular domain.

Depositor

Clare Slaney

Article

Zhang et al Clin Transl Immunology. 2025 Aug 21;14(8

Insert

antiFLAG-CAR4

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Promoter

EF-1α

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pBOB-HA-Gna15

Plasmid

#246003

Purpose

Express HA N-terminal tagged mouse Galpha15 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gna15 (Gna15 Mouse)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pBOB-HA-Gna14

Plasmid

#246002

Purpose

Express HA N-terminal tagged mouse Galpha14 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gna14 (Gna14 Mouse)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pBOB-HA-Gnaz

Plasmid

#246000

Purpose

Express HA N-terminal tagged mouse Galphaz transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gnaz (Gnaz Mouse)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-Gpr45

Plasmid

#245982

Purpose

Express wild-type mouse GPR45 transiently via transfection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gpr45 (Gpr45 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-Gnao1-B

Plasmid

#245999

Purpose

Express HA N-terminal tagged mouse GalphaoB transiently via transfection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gnao1 (Gnao1 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-Gpr45-Y287C

Plasmid

#245983

Purpose

Express mouse GPR45-Y287C mutant transiently via transfection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gpr45 (Gpr45 Mouse)

Expression

Mammalian

Mutation

Y287C

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-Gpr45-S214P

Plasmid

#245984

Purpose

Express mouse GPR45-S214P mutant transiently via transfection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gpr45 (Gpr45 Mouse)

Expression

Mammalian

Mutation

S214P

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-Gpr45-3xFlag

Plasmid

#245985

Purpose

Express 3xFlag C-terminal tagged wild-type mouse GPR45 transiently via transfection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gpr45 (Gpr45 Mouse)

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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PiggyBac EF1a-eGFP-Puro U6 MMP2 g2

Plasmid

#170825

Purpose

PiggyBac Cas13d sgRNA plasmid for MMP2 knockdown

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Cas13d MMP2 gRNA2

Use

Piggybac transposon

Expression

Mammalian

Promoter

U6

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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PiggyBac EF1a-eGFP-Puro U6 ANXA2 g1

Plasmid

#170826

Purpose

PiggyBac Cas13d sgRNA plasmid for ANXA2 knockdown

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Cas13d ANXA2 gRNA1

Use

Piggybac transposon

Expression

Mammalian

Promoter

U6

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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PiggyBac EF1a-eGFP-Puro U6 ANXA2 g2

Plasmid

#170827

Purpose

PiggyBac Cas13d sgRNA plasmid for ANXA2 knockdown

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Cas13d ANXA2 gRNA2

Use

Piggybac transposon

Expression

Mammalian

Promoter

U6

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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PiggyBac EF1a-eGFP-Puro U6 CLCN3 g1

Plasmid

#170828

Purpose

PiggyBac Cas13d sgRNA plasmid for CLCN3 knockdown

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Cas13d CLCN3 gRNA1

Use

Piggybac transposon

Expression

Mammalian

Promoter

U6

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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PiggyBac EF1a-eGFP-Puro U6 CLCN3 g2

Plasmid

#170829

Purpose

PiggyBac Cas13d sgRNA plasmid for CLCN3 knockdown

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Cas13d CLCN3 gRNA2

Use

Piggybac transposon

Expression

Mammalian

Promoter

U6

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgWAVE2-2

Plasmid

#210136

Purpose

knock out WAVE2 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Actin-binding protein WASF2 (WASF2 Human)

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgWAVE2-1

Plasmid

#210135

Purpose

knock out WAVE2 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Actin-binding protein WASF2 (WASF2 Human)

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pHD22y/PfPRMT5-PTP

Plasmid

#203160

Purpose

for tagging PTP to the 5' end of PfPRMT5 gene by single crossover

Depositor

Liwang Cui

Article

Lucky et al Commun Biol. 2023 Jun 22;6(1):659. doi:

Insert

PfPRMT5 fragment, PTP tag and pDT 3' UTR

Tags

3' UTR of P. berghei dihydrofolate reductase…

Expression

Mammalian

Promoter

No

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-miniSdd3-BE4max-BlastR

Plasmid

#204849

Purpose

For cytosine base editing using miniSdd3 in mammalian cells

Depositor

Caixia Gao

Article

Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

Insert

miniSdd3-nSpCas9(D10A)-UGI

Use

CRISPR

Expression

Mammalian

Mutation

D10A in SpCas9

Promoter

CMV, SV40, EM7

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pGW02 AAV-TBG-Cre-sgP53-sgRNA

Plasmid

#192162

Purpose

AAV-TBG-Cre-sgP53-sgRNA

Depositor

Sidi Chen

Article

Wang et al Cancer Discov. 2020 Dec;10(12):1912-1933

Type

Empty backbone

Use

AAV

Mutation

NA

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-hFAM3B-HA

Plasmid

#180145

Purpose

Coding sequence of human FAM3B pCS2 with C terminal HA tag

Depositor

Edward De Robertis

Article

Zhang et al Proc Natl Acad Sci U S A. 2021 May 18;11

Insert

FAM3B (FAM3B Human)

Tags

HA

Expression

Mammalian

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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