We narrowed to 13,449 results for: Rras
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Plasmid#198668PurposeAn Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiaeDepositorInsertXI2_DW
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.H02L
Plasmid#198667PurposeAn Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiaeDepositorInsertXI2_UP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.H01L
Plasmid#198665PurposeAn Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiaeDepositorInsertX3_UP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCK005 ubi:mCerulean_ubb-polyA
Plasmid#195979PurposeExpression constructDepositorInsertubi:mCerulean
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNHT7
Plasmid#186753PurposeA basic cloning vector for inserting genes with 5' and 3' UTRs for later subcloning via Golden Gate assembly.DepositorTypeEmpty backboneUseSynthetic Biology; Cloning backboneTagsExpressionMutationPromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-hGFAP-ND1-K139D-T2A-mCherry
Plasmid#178587PurposeExpression of NEUROD1-K139D and mCherry under the human GFAP promoterDepositorInsertNEUROD1-K139D (NEUROD1 Human)
UseAAVTagsExpressionMammalianMutationNEUROD1-K139DPromoterAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-hGFAP-ND1-R111L-T2A-mCherry
Plasmid#178586PurposeExpression of NEUROD1-R111L and mCherry under the human GFAP promoterDepositorInsertNEUROD1-R111L (NEUROD1 Human)
UseAAVTagsExpressionMammalianMutationNEUROD1-R111LPromoterAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-udhA-xylA
Plasmid#158612PurposeLow phosphate inducible gRNA array to silence the xylA and udhA promotersDepositorInsertudhA-xylA guide array
UseTagsExpressionBacterialMutationPromoterE . coli ugpB gene promoter, low phosphate inductā¦Available sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-gltA2-xylA
Plasmid#158613PurposeLow phosphate inducible gRNA array to silence the xylA and gltA2 promotersDepositorInsertgltA2-xylA guide array
UseTagsExpressionBacterialMutationPromoterE . coli ugpB gene promoter, low phosphate inductā¦Available sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-zwf-xylA
Plasmid#158614PurposeLow phosphate inducible gRNA array to silence the xylA and zwf promotersDepositorInsertzwf-xylA guide array
UseTagsExpressionBacterialMutationPromoterE . coli ugpB gene promoter, low phosphate inductā¦Available sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
DT048A
Plasmid#159409PurposeConstitutive LuxR expression, controlled under J23101 E.coli promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoterDepositorInsertpr.J23101-LuxR-pr.LuxR-PFO
UseSynthetic BiologyTagsFLAG/FLAGExpressionBacterialMutationPromoterJ23101 and pLux promoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
DT046A
Plasmid#159407PurposeConstitutive LuxR expression, controlled under E.coli tet promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoterDepositorInsertpr.tet-LuxR-pr.LuxR-PFO
UseSynthetic BiologyTagsFLAG/FLAGExpressionBacterialMutationPromotertet and pLux promoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
DT047A
Plasmid#159408PurposeConstitutive LuxR expression, controlled under T7 bacteriophage promoter, and inducible PFO-sfGFP chimeric protein expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoterDepositorInsertpr.T7-LuxR-pr.LuxR-sfGFP-PFO
UseSynthetic BiologyTagsFLAG/FLAGExpressionBacterialMutationPromotertet and pLux promoterAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1972 - [1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)
Plasmid#159844PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2301 - [1-2] ENTR - Fluor - ce-mMaple3(1 intron, syntrons(3), no_atg, no_stop)
Plasmid#159865PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-mMaple3(1 intron, syntrons(3), no_atg, no_stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAWH-IRP
Plasmid#133896PurposePositive control when used with pMS22H-IRE. Expression of Gal4AD-IRP hybrid protein. Homology regions for recombination with pMS22HDepositorInsertIRP
UseTagsExpressionYeastMutationPromoterT7Available sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_X_AsPT1-LOV2-EcSsra
Plasmid#131217PurposeExpresses AsPT1-LOV2-EcSsra (Avena Sativa) preceded by restriction sitesDepositorInsertAsPT1-LOV2-EcSsra
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_X_AsPT1-LOV2-pep
Plasmid#131215PurposeExpresses AsPT1-LOV2-pep (Avena sativa) preceded by restriction sitesDepositorInsertAsPT1-LOV2-pep
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pK RagA(R37P)-RagC
Plasmid#99706PurposeBacterial expression of RagA(R37P)-RagCDepositorUseTagsHis tag, Poly Arg, and SUMOExpressionBacterialMutationR37PPromoterT7Available sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pK RagA-RagC(L91P)
Plasmid#99707PurposeBacterial expression of RagA-RagC(L91P)DepositorUseTagsHis tag, Poly Arg, and SUMOExpressionBacterialMutationL91PPromoterT7Available sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pK RagA-RagC(K84T)
Plasmid#99708PurposeBacterial expression of RagA-RagC(K84T)DepositorUseTagsHis tag, Poly Arg, and SUMOExpressionBacterialMutationK84TPromoterT7Available sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETDuet RagA(R37P)-RagC
Plasmid#99646PurposeBacterial expression of RagA(R37P)-RagCDepositorUseTagsHis tagExpressionBacterialMutationR37PPromoterAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETDuet RagA-RagC(K84T))
Plasmid#99648PurposeBacterial expression of RagA-RagC(K84T)DepositorUseTagsHis tagExpressionBacterialMutationK84TPromoterAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pK RagA(T21N)-RagC
Plasmid#99650PurposeBacterial expression of RagA(T21N)-RagCDepositorUseTagsHis tag, Poly Arg, and SUMOExpressionBacterialMutationT21NPromoterAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pK RagA-RagC(S75N)
Plasmid#99699PurposeBacterial expression of RagA-RagC(S75N)DepositorUseTagsHis tag, Poly Arg, and SUMOExpressionBacterialMutationS75NPromoterAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV7.1-Flag-NLS-hSPDEF delta2
Plasmid#100871PurposeMammalian expression of human SPDEF truncationDepositorInsertSPDEF (SPDEF Human)
UseTags3xFLAGExpressionMammalianMutationtruncated protein 71-246aaPromoterCMVAvailable sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER20-Flag-NLS-hSPDEF delta3
Plasmid#100876PurposeLentiviral expression of human SPDEF truncationDepositorInsertSPDEF (SPDEF Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationtruncated protein 243-335aaPromoterTRE2Available sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV7.1-Flag-NLS-hSPDEF delta3
Plasmid#100872PurposeMammalian expression of human SPDEF truncationDepositorInsertSPDEF (SPDEF Human)
UseTags3xFLAGExpressionMammalianMutationtruncated protein 244-335aaPromoterCMVAvailable sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV7.1-Flag-NLS-hSPDEF delta1
Plasmid#100870PurposeMammalian expression of human SPDEF truncationDepositorInsertSPDEF (SPDEF Human)
UseTags3xFLAGExpressionMammalianMutationtruncated protein 1-248aaPromoterCMVAvailable sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Alx3
Plasmid#80382Purposemammalian expression of rat Alx3DepositorInsertAlx3 (Alx3 Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
C12-bio-His
Plasmid#52178PurposeExpresses full-length Spexin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertC12 (SPX Human)
UseTagsHis tag, enzymatic biotinylation sequence, and raā¦ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET14b-PITPalpha N90F
Plasmid#58257Purposebacterial expression of human PITP alpha N90FDepositorInsertPITP alpha (PITPNA Human)
UseTagsHISExpressionBacterialMutationN90F (abolishes PI binding)PromoterT7Available sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET14b-PITPalpha K61A
Plasmid#58258Purposebacterial expression of human PITP alpha K61ADepositorInsertPITP alpha (PITPNA Human)
UseTagsHISExpressionBacterialMutationK61A (abolishes PI binding)PromoterT7Available sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLEX_970_puro_DEST_V5_TLOC1 (220aa)
Plasmid#45287DepositorInsertTLOC1 (SEC62 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
hs-FL-Kek1-myc2
Plasmid#41170DepositorInsertKek1 (kek1 Fly)
UseTags2X mycExpressionInsectMutationPromoterHsp70Available sinceJan. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pIS57 PGM2 3'UTR mut
Plasmid#14492DepositorInsertPGM2 3'UTR mutant (PGM2 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationCATTCCās were changed to AAGTAC'sPromoterAvailable sinceMarch 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pIS56 NETO2 3'UTR mut
Plasmid#14496DepositorInsertNETO2 3'UTR mutant (NETO2 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationCATTCCās were changed to AAGTAC'sPromoterAvailable sinceMarch 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pIS55 POGK 3'UTR mut
Plasmid#14498DepositorInsertPOGK 3'UTR mutant (POGK Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationFirst, fourth, and fifth occurrences of CATTCCās ā¦PromoterAvailable sinceMarch 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pIS53 SERP1 3'UTR mut
Plasmid#14500DepositorInsertSERP1 3'UTR mutant (SERP1 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationCATTCCās were changed to AAGTAC'sPromoterAvailable sinceMarch 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
LLP457 pGK-dCas9-Suntag-BFP
Plasmid#100957PurposeBPF version of LLP339, Puro is replaced by BFP driven by EF1a, dCas9 with SungTagx10 driven by pGK, with 3xHA tagDepositorInsertdCas9, SunTag array (10xGCN4)
UseCRISPRTags3xHA and BFPExpressionMammalianMutationD10A, H840A for dCas9PromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
G608G lamin A minigene
Plasmid#20292DepositorInsertlamin A (LMNA Human)
UseTagsGFPExpressionMammalianMutationG606G:GGC>GGT Mutation causes aberrant spliā¦PromoterAvailable sinceMarch 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
TKTL1
Plasmid#72419PurposeStudy the role of aberrant expression of TKTL1 in HNSCC tumorigenesisDepositorInsertTKTL1 (TKTL1 Human)
UseTagsExpressionMammalianMutationmay have one or two synonymous substitutionsPromoterCMVAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-tagBFP-TetR
Plasmid#103797PurposeBLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorUseTagsExpressionMammalianMutationTetR: A4G (M2V)PromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDF-casBCDE(g8)
Plasmid#89727PurposeExpresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing 33-nt spacer (referred to as 'wt') matching a protospacer in the M13 phage genome.DepositorInsertCRISPR array
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-TetR-tagRFP-T
Plasmid#103809Purpose'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitmentDepositorUseTagsExpressionMammalianMutationTetR: A4G (M2V)PromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-EYFP-TetR
Plasmid#103798PurposeBLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorUseTagsExpressionMammalianMutationTetR: A4G (M2V)PromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
zgc:153086_R (OZ512)
Plasmid#27187DepositorInsertZinc finger array targeting zgc:153086
UseZebrafish targetingTagsExpressionMutationPromoterAvailable sinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
zgc:153086_L (OZ511)
Plasmid#27186DepositorInsertZinc finger array targeting zgc:153086
UseZebrafish targetingTagsExpressionMutationPromoterAvailable sinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
SDC3_L (OZ595)
Plasmid#35235DepositorInsertZinc finger array targeting SDC3
UseZebrafish targetingTagsExpressionMutationPromoterAvailable sinceMarch 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
SDC3_R (OZ596)
Plasmid#35236DepositorInsertZinc finger array targeting SDC3
UseZebrafish targetingTagsExpressionMutationPromoterAvailable sinceMarch 5, 2012AvailabilityAcademic Institutions and Nonprofits only