We narrowed to 5,155 results for: PEP

1. pCDH-UTY-F

   Plasmid

   #40617

   Depositor

   Kai Ge

   Article

   Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.

   Insert

   UTY (UTY Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mutation

   Promoter

   EF1

   Available since

   Oct. 11, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pET28a-SpyTag002-MBP

   Plasmid

   #102831

   Purpose

   Bacterial expression of SpyTag002 fused to maltose binding protein

   Depositor

   Mark Howarth

   Article

   Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

   Insert

   SpyTag002-MBP
   

   Use

   Tags

   His6 and Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   Peptide tag added to the N-terminus of MBP via a …

   Promoter

   T7

   Available since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-mCherry-4A/4B-CAAX

   Plasmid

   #87699

   Purpose

   Express mCherry with a peptide linker (contaning a HCVp 4A/4B substrate site) and a C-terminal CAAX box from KRras for plasma-membrane targeting in mammalian cells

   Depositor

   Robert Campbell

   Article

   Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

   Insert

   mCherry-4A/4B-CAAX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. OA984

   Plasmid

   #120363

   Purpose

   Expresses an single chain antibody 1C19 that targets all four DENV serotypes and expresses a red fluorescent protein reporter

   Depositor

   Omar Akbari

   Article

   Buchman et al PLoS Pathog. 2020 Jan 16;16(1):e1008103.

   Insert

   1C19 single chain antibody
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   AAEL010782 carboxypeptidase A (CPA) gene Promoter

   Available since

   Feb. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEHlyA5

   Plasmid

   #168298

   Purpose

   Secretion of nanobodies and other polypeptides fused to C-terminal secretion signal of HlyA

   Depositor

   Luis Ángel Fernández

   Article

   Ruano-Gallego et al Microb Cell Fact. 2019 Mar 11;18(1):47.

   Insert

   N‑terminal His tag, VHH, HA and E‑tags, C‑HlyA
   

   Use

   Tags

   E-tag, HA-tag, His-tag, Prescission site, and VHH…

   Expression

   Bacterial

   Mutation

   Promoter

   LacI-Plac

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Dimer cleaved

   Plasmid

   #198199

   Purpose

   FRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 2A peptide linker for posttranslational linker cleavage

   Depositor

   Christian Wahl-Schott

   Article

   Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

   Insert

   mVenus-T2A-mTurquoise2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A206K both

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. LifeAct-MTED

   Plasmid

   #217498

   Purpose

   Expresses LifeAct-mScarlet-MTED for targeting fluorescent, MT depolymerizing peptide MTED to f-actin

   Depositor

   Erik Dent

   Article

   Holland et al Mol Biol Cell. 2024 Jun 1;35(6):mr3. doi

   Insert

   LifeAct-mScarlet-MTED
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVasa-Cre

   Plasmid

   #15885

   Depositor

   Diego Castrillon

   Article

   Gallardo et al Genesis. 2007 Jun . 45(6):413-7.

   Insert

   mouse vasa promoter (Ddx4 Mouse)
   

   Use

   Tags

   Cre

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. mVenus-hCOL1A1

   Plasmid

   #140111

   Purpose

   Expresses fluorescently-tagged human Type I procollagen α1 chain with mVenus replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

   Depositor

   Sergey Leikin

   Insert

   Type I procollagen α1 (COL1A1 Human)
   

   Use

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   COL1A1 exons 2-3 replaced by fluorescent tag

   Promoter

   CMV

   Available since

   May 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-fNPY-GFP

    Plasmid

    #22912

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 . 3():19.

    Insert

    fugu neuropeptide Y promoter driving GFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. mApple-hCOL1A1

    Plasmid

    #140113

    Purpose

    Expresses fluorescently-tagged human Type I procollagen α1 chain with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

    Depositor

    Sergey Leikin

    Insert

    Type I procollagen α1 (COL1A1 Human)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    COL1A1 exons 2-3 replaced by fluorescent tag

    Promoter

    CMV

    Available since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. mNFAT4-462:TALE:VP64:KRɸ

    Plasmid

    #160662

    Purpose

    Constitutive expression of murine NFAT (aa 4-462) fused to the DNA-binding domain from TALEN1257, the VP64 activation domain and the membrane anchor peptide KRɸ.

    Depositor

    Roman Jerala

    Article

    Mesko et al ACS Synth Biol. 2020 Aug 21;9(8):2055-20

    Insert

    mNFAT4-462:TALE:VP64:KRɸ
    

    Use

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX-2T-RNAPII-CTD wt #14-HA

    Plasmid

    #160688

    Purpose

    Expression of GST-RNA Polymerase II C-terminal domain fusion protein. Contains 14 of the consensus heptapeptide repeats shown to be phosphorylated by Cdc2 kinase.

    Depositor

    Jeffry Corden

    Article

    Patturajan et al J Biol Chem. 1998 Feb 20;273(8):4689-94.

    Insert

    RNA Polymerase II CTD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C terminal domain only

    Promoter

    Available since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDS221

    Plasmid

    #34981

    Purpose

    Expresses a high-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    ePDZb1–mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST14-Avitag-SpyCatcher002

    Plasmid

    #102828

    Purpose

    Bacterial expression of SpyCatcher002 with a peptide tag enabling site-specific biotinylation

    Depositor

    Mark Howarth

    Article

    Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

    Insert

    Avitag-SpyCatcher002
    

    Use

    Tags

    Avitag, GSSGS, His6, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV_PACLight1

    Plasmid

    #197864

    Purpose

    Mammalian expression of PACLight1 biosensor for PACAP (Pituitary Adenylate Cyclase Activating Peptide)

    Depositor

    Tommaso Patriarchi

    Article

    Cola et al Elife. 2024 Aug 15;13:RP96496. doi: 10.7

    Insert

    PACLight1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. Clathrin-LCa-EYFP

    Plasmid

    #21741

    Depositor

    Xiaowei Zhuang

    Article

    Chen et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

    Insert

    Clathrin, light polypeptide (LCa) (Clta Mouse)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

18. UbG76V-eGFP-V5His_20aa

    Plasmid

    #23970

    Depositor

    Nico Dantuma

    Article

    Beskow et al J Mol Biol. 2009 Dec 11. 394(4):732-46.

    Insert

    Ub-G76V-eGFP-V5HIS_20aa
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    G76V mutation in Ubiquitin. Ub fused to N-termin…

    Promoter

    Available since

    March 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNK869

    Plasmid

    #219737

    Purpose

    MoClo-compatible Level 0 - promoter + 5U vector carrying promoter AtPD7

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

    Insert

    promoter of serine carboxypeptidase-like gene AtSCPL30, AtPD7
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtPD7

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(+)-PKI-GFP

    Plasmid

    #118482

    Purpose

    GFP-tagged Protein Kinase A inhibitor peptide.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    PKI-GFP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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