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KIF13B 1-584

Plasmid

#163908

Purpose

Motile fragment of mouse KIF13B (aa 1-584) for purification from insect cells.

Depositor

Martin Loose

Article

Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

kif13b (Kif13b Mouse)

Tags

Cleavable 6 x HIS tag

Expression

Insect

Promoter

polyhedrin

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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mWasabi-ADAP1

Plasmid

#163906

Purpose

Full length mouse ADAP1 with N terminal fluorescent tag for purification from insect cells

Depositor

Martin Loose

Article

Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

ADAP1 (Adap1 Mouse)

Tags

mWasabi fluorescent tag

Expression

Insect

Promoter

polyhedrin

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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p8115 LentiCRISPR v2 sgPTPN14-3

Plasmid

#163314

Purpose

Expresses Cas9 and a human PTPN14-targeting control guide RNA

Depositor

Elizabeth White

Article

Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

Insert

sgPTPN14-3

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-Myosin7B-sgRNA

Plasmid

#154861

Purpose

Lentiviral expression of Cas9 and sgRNA targeting Myosin7B

Depositor

Yihong Ye

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

Insert

MYO7B sgRNA (MYO7B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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tol2-lyzC-miR-23a-2-dendra2

Plasmid

#105435

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

miR-23a-2 Dendra2 (mir23a-2 Zebrafish)

Use

Zebrafish

Tags

N/A

Available Since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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hsa-cdk2 3'UTR sicheck2

Plasmid

#121125

Purpose

luciferase assay

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

hsa-cdk2 3'UTR (CDK2 Human)

Expression

Bacterial

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pDM304_YFP-IqgC

Plasmid

#128697

Purpose

expression of YFP-tagged IqgC in Dictyostelium discoideum cells

Depositor

Igor Weber

Article

Marinovic et al Proc Natl Acad Sci U S A. 2019 Jan 22;11

Insert

iqgC

Use

Dictyostelium discoideum expression vector

Tags

YFP

Mutation

none

Available Since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_SNORD2 probe

Plasmid

#73070

Purpose

antisense probe for detection of human SNORD2 (C/D box snoRNA snR39B)

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

SNORD2 (SNORD2 Human)

Expression

Mammalian

Promoter

t7

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_SNORD60 probe

Plasmid

#73071

Purpose

antisense probe for detection of human SNORD60 (C/D box snoRNA U60)

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

SNORD60 (SNORD60 Human)

Expression

Mammalian

Promoter

t7

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only
Gal4-HSF4b K294R

Plasmid

#118359

Purpose

This plasmid expresses amino-acids 200-493 of hHSF4b K294R mutant fused to the GAL-4 DNA binding domain. This construct cannot undergo sumoylation on K294.

Depositor

Lea Sistonen

Article

Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

Insert

hHSF4b (HSF4 Human)

Expression

Mammalian

Mutation

K294R

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pOU2

Plasmid

#41754

Depositor

Jeff Gelles

Article

Martin et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

kinesin heavy chain (Khc Fly, E. coli)

Tags

His6 and bccp

Expression

Bacterial

Mutation

residues 1-401; C45S C338S C451S

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pHW564 (Pmyo-2::cGAL-N(C1A)::let-858 3'UTR)

Plasmid

#107739

Purpose

mutated cGAL-N (C1A) driver under the control of C. elegans myo-2 promoter

Depositor

Paul Sternberg

Article

Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

Insert

NLS::cGAL(DBD)::gp41-1-N-intein(C1A)

Expression

Worm

Mutation

change the first cysteine of gp41-1 N-intein to a…

Promoter

C. elegans myo-2 promoter

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pMGF130

Plasmid

#96971

Purpose

S. pombe lem2 knockout cassette with hygro resistance gene

Depositor

Adam Frost, Wesley Sundquist

Article

Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

lem2 (lem2 Fission Yeast)

Expression

Bacterial

Mutation

whole ORF deletion and replaced with hygro resist…

Promoter

N/A

Available Since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pMGF172

Plasmid

#96998

Purpose

ish1 mCherry insertion cassette with ura4+ gene

Depositor

Adam Frost, Wesley Sundquist

Article

Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

ish1 (ish1 Fission Yeast)

Tags

GST and mCherry

Expression

Bacterial

Mutation

ish1 C-terminal domain fused with mCherry + ura4(…

Promoter

N/A

Available Since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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GST-CHMP4A (205-222) (pGEX2T(TEV))

Plasmid

#80633

Purpose

Residues 205-222 of CHMP4A, Bacterial Expression Vector; TEV-cleavable GST tag; Sundquist Lab Internal ID: WISP06-60

Depositor

Wesley Sundquist

Article

McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

Insert

CHMP4A (CHMP4A Human)

Tags

GST

Expression

Bacterial

Mutation

Residues 205-222;

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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213-petDuet1-GBD-LUMP--cdc42

Plasmid

#65973

Purpose

Dual expression of GBD-LUMP and cdc42

Depositor

Gerard Marriott

Article

Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p

Inserts

LUMP
cell division cycle 42 (CDC42 Human)

Tags

GBD (GTPase binding domain from ACK1; residues 44…

Expression

Bacterial

Mutation

constitutively active Cdc42 (Q61L)

Promoter

T7

Available Since

July 13, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR223 PAK5 P50L

Plasmid

#60533

Purpose

Donor vector for human Pak5 P50L mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway donor vector

Mutation

P50L

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223 PAK5 P76T

Plasmid

#60534

Purpose

Donor vector for human Pak5 P76T mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway donor vector

Mutation

P76T

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223 PAK5 S312C

Plasmid

#60535

Purpose

Donor vector for human Pak5 S312C mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway donor vector

Mutation

S312C

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223 PAK5 V463L

Plasmid

#60536

Purpose

Donor vector for human Pak5 V463L mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway donor vector

Mutation

V463L

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only