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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,041 results for: pET

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Showing: 3561 - 3580 of 10041 results

  1. CBX4

    Plasmid
    #25237
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CBX4_02 (CBX4 Human)
    Tags
    6x His and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by

  2. MZB211

    Plasmid
    #228494
    Purpose
    10xHis-SUMO (Smt3) tagged Ssod7-fused Pfu Polymerase with fidelity enhancing mutation (E. coli based protein expression)
    Depositor
    Richard Baker
    Insert
    pol
    Tags
    10xHis-SUMO(Smt3)
    Expression
    Bacterial
    Mutation
    A408S
    Promoter
    T7 Promoter
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    228494-plasmid-map-sequence-id-455979

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  3. K9L mutant of H3K9me3 biosensor

    Plasmid
    #120807
    Purpose
    FRET biosensor. Eliminating the H3 lysine 9 methylation site in H3K9me3 biosensor to abolish the detection capability
    Depositor
    Yingxiao Wang
    Publication
    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Truncated YPet-HP1-EV linker-ECFP-mouse histone H3(K9L mutation)
    Expression
    Mammalian
    Mutation
    lysine 9 is mutated to leucine 9 on histone H3
    Promoter
    CMV
    Available since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    120807-plasmid-map-sequence-id-235134

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  6. USP5

    Plasmid
    #25299
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    USP5.001.835 (USP5 Human)
    Tags
    His
    Expression
    Bacterial
    Available since
    Aug. 16, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by

  8. ACAT1

    Plasmid
    #25510
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ACAT1 (ACAT1 Human)
    Tags
    His
    Expression
    Bacterial
    Available since
    Aug. 30, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by

  10. MYST1

    Plasmid
    #25180
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    MYST1_01 (KAT8 Human)
    Tags
    His
    Expression
    Bacterial
    Available since
    Aug. 9, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by

  13. Dead Streptavidin-SpyTag

    Plasmid
    #59548
    Purpose
    pET21-DTag encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyTag, for generation of chimeric SpyAvidin tetramers
    Depositor
    Mark Howarth
    Publication
    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-
    Insert
    Dead Streptavidin-SpyTag
    Tags
    SpyTag
    Expression
    Bacterial
    Mutation
    The construct lacks final K of SpyTag
    Promoter
    T7
    Available since
    Oct. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  14. pKK-TEV-mClover3

    Plasmid
    #105795
    Purpose
    Expression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV). mClover3 is a brighter derivative of mEGFP (PMID: 26879144).
    Depositor
    Andrzej Dziembowski
    Publication
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-mClover3
    Expression
    Mammalian
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    105795-plasmid-map-sequence-id-203386

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  15. pKK-TEV-ProteinA

    Plasmid
    #105788
    Purpose
    Expression of your protein of interest in fusion with two IgG binding domains of Staphylococcus aureus protein A at the C-terminus (cleavable by TEV) (PMID: 3507693, 10504710). Protein purification.
    Depositor
    Andrzej Dziembowski
    Publication
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-ProteinA
    Expression
    Mammalian
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    105788-plasmid-map-sequence-id-203379

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  17. DOT1L

    Plasmid
    #40736
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    DOT1L (DOT1L Human)
    Tags
    6xHis TEV
    Expression
    Bacterial
    Available since
    Oct. 18, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by

  18. NR4A1

    Plasmid
    #25477
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NR4A1 (NR4A1 Human)
    Tags
    His
    Expression
    Bacterial
    Available since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
Showing: 3561 - 3580 of 10041 results