Addgene

We narrowed to 504 results for: Chuk

Showing: 341 - 360 of 504 results

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Chr1q_Cassette-Integration_gRNA

Plasmid

#195126

Purpose

gRNA in a third generation Cas9 vector with GFP, targeting location downstream of Chr1q_centromere-targeting_gRNA for positive-negative selection cassette integration

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Chr1q gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-Puro-PFDN2

Plasmid

#202664

Purpose

Tet-ON 3rd generation expression vector with inducible expression of PFDN2 and a puromycin selection cassette

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

prefoldin subunit 2 (PFDN2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynapsin.SF-iGluSnFR.A184V

Plasmid

#106175

Purpose

Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184V

Use

AAV

Tags

Expression

Mutation

GltI: A184V

Promoter

hSynapsin

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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MAC-CTSS

Plasmid

#172415

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

Cathepsin S (CTSS Human)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Nov. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pET-His6-SUMO-MproC145A

Plasmid

#172653

Purpose

Bacterial expression of SARS-CoV-2 Mpro C145A inactive mutant fused to SUMO

Depositor

Stephen Harrison

Article

MacDonald et al ACS Infect Dis. 2021 Sep 10;7(9):2591-25

Insert

SARS-CoV-2 Mpro (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Use

Tags

6xHis Tag and SUMO

Expression

Bacterial

Mutation

Catalytic Cysteine 145 mutated to Alanine

Promoter

T7

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap-FLEX.SF-iGluSnFR.S72A

Plasmid

#106182

Purpose

Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.S72A

Use

AAV

Tags

Expression

Mutation

GltI: S72A

Promoter

hSynapsin

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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MAC-CLEC4E

Plasmid

#172412

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

C-type lectin domain family 4 member E (CLEC4E Human)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Nov. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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OR2M4_Deletion_gRNA

Plasmid

#195194

Purpose

dual gRNAs for deletion of OR2M4 in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

OR2M4 dual gRNA (OR2M4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG-FLEX.SF-iGluSnFR.A184V

Plasmid

#106187

Purpose

Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184V

Use

AAV

Tags

Expression

Mutation

GltI: A184V

Promoter

CAG

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.SF-iGluSnFR.A184V

Plasmid

#106193

Purpose

Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184V

Use

AAV

Tags

Expression

Mutation

GltI: A184V

Promoter

GFAP

Available since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.SF-iGluSnFR.S72A

Plasmid

#106194

Purpose

Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.S72A

Use

AAV

Tags

Expression

Mutation

GltI: S72A

Promoter

GFAP

Available since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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mito-yHS1-M7A,H102A

Plasmid

#159165

Purpose

Mitochondrial labile heme reporter

Depositor

Amit Reddi

Article

Martinez-Guzman et al J Cell Sci. 2020 May 20;133(10). pii: jc

Insert

mito-yHS1-M7A,H102A

Use

Tags

COX4 pre-sequence

Expression

Yeast

Mutation

Mutated both Met 7 and His 102 of the cyt b562 mo…

Promoter

GPD

Available since

Sept. 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV.CAG-FLEX.SF-iGluSnFR.S72A

Plasmid

#106188

Purpose

Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.S72A

Use

AAV

Tags

Expression

Mutation

GltI: S72A

Promoter

CAG

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pNK7185

Plasmid

#219765

Purpose

Vector for expression PzPKS2 in yeast (P.pastoris)

Depositor

Karen Sarkisyan

Article

Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

Insert

PzPKS2

Use

Tags

Expression

Yeast

Mutation

Promoter

pGAP

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pNK7186

Plasmid

#219758

Purpose

Vector for expression PpASCL in mammalian cells (HEK293NT)

Depositor

Karen Sarkisyan

Article

Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

Insert

PpASCL

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pNK7179

Plasmid

#219757

Purpose

Vector for expression HmS in mammalian cells (HEK293NT)

Depositor

Karen Sarkisyan

Article

Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

Insert

HmS

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV4-HA-p31(comet)-S102A

Plasmid

#221698

Purpose

Mammalian expression of HA-tagged human p31(comet) (S102A)

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

MAD2BP1

Use

Tags

Expression

Mammalian

Mutation

S102A

Promoter

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-RNF8-R42A

Plasmid

#221702

Purpose

Lentiviral expression of human RNF8 (R42A) in mammalian cells

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

RNF8 (RNF8 Human)

Use

Lentiviral

Tags

Expression

Mutation

R42A

Promoter

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-RNF8-C403S

Plasmid

#221703

Purpose

Lentiviral expression of human RNF8 (C403S) in mammalian cells

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

RNF8 (RNF8 Human)

Use

Lentiviral

Tags

Expression

Mutation

C403S

Promoter

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-RNF8-S157A

Plasmid

#221704

Purpose

Lentiviral expression of human RNF8 (S157A) in mammalian cells

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

RNF8 (RNF8 Human)

Use

Lentiviral

Tags

Expression

Mutation

S157A

Promoter

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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