Addgene

We narrowed to 2,116 results for: PAM

Showing: 341 - 360 of 2116 results

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pLenti-EF1a-CDK6-mutPAM-PGK-puro

Plasmid

#187638

Purpose

sg resistant CDK6 ORF

Depositor

William Kaelin

Article

Nicholson et al Sci Signal. 2019 Oct 1;12(601). pii: 12/

Insert

CDK6 (CDK6 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

PAM site mutation

Promoter

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pJT311 AAV-Rapamycin Inducible-NZF-Citrine

Plasmid

#202060

Purpose

Inducible AAV activator

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

AAV-Rapamycin Inducible-NZF-Citrine

Use

AAV; Aav with reporter

Tags

Expression

Mammalian

Mutation

ITR deletion

Promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pAM-DCA-HA-Ast-3-NP-1-IRES-mCherry-WPRE

Plasmid

#159630

Purpose

AAV expression of HA Allatostatin-3, neurophysin, IRES mCherry under the CAG promoter

Depositor

Ross Bathgate, Andrew Allen

Article

Ngo et al Cell Rep. 2020 Sep 15;32(11):108139. doi

Insert

Ast (AstA Fly)

Use

AAV

Tags

HA, IRES, mCherry, and neurophysin-1

Expression

Mammalian

Mutation

Promoter

DCA (same as CAG promoter)

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated

Plasmid

#215769

Purpose

encodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locus

Depositor

Dimitrios Wagner

Article

Glaser et al Genome Biol. 2023 Apr 24;24(1):89. doi:

Inserts

TRAC-5' homology arm (TRAC Human)
P2A-CD19.28.zeta-bgH (CD19 Mouse, Human, Bovine)
TRAC-3' homology arm (TRAC Human)

Use

Tags

Expression

Bacterial

Mutation

Cas12a PAM mutated

Promoter

Available since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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pJT315 AAV-Rapamycin Inducible-ZNF473KRAB-Citrine

Plasmid

#202064

Purpose

Inducible AAV activator

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

AAV-Rapamycin Inducible-ZNF473KRAB-Citrine

Use

AAV; Aav with reporter

Tags

Expression

Mammalian

Mutation

ITR deletion

Promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGW182-PAM26B-F1370A-dsRNAres_L

Plasmid

#146838

Purpose

Insect Expression of DmGW182-PAM26B-F1370A-dsRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmGW182-PAM26B-F1370A-dsRNAres (gw Fly)

Use

Tags

Expression

Insect

Mutation

Two non silent mutations V704A and N799S and one …

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAM/CBA-eGFP-miRNegativex3-WPRE-bGHpA

Plasmid

#194249

Purpose

Expresses 3 non-targeting miRNAs

Depositor

Michael J Fox Foundation MJFF

Insert

miRNegative

Use

AAV and RNAi

Tags

Expression

Mutation

Promoter

CAG

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-Ef1A-Myc-POLB(PAMmut)

Plasmid

#176150

Purpose

N-terminus Myc-tagged POLB containing a mutation in the PAM site used by POLB gRNA1 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

Mutation in the PAM site used by POLB gRNA1

Promoter

EF1A

Available since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Dopamine D2 receptor [N186/29R] (Recombinant Antibody)

Antibody

#184204-rAb

Purpose

Anti-Dopamine D2 receptor (Rat) recombinant mouse monoclonal antibody. Recognizes long and short forms.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Recommended Applications

Immunohistochemistry

Reactivity

Mouse and Rat

Source Species

Mouse

Isotype

IgG2a

Trial Size

Available to purchase

Available since

Aug. 22, 2022

Availability

Industry, Academic Institutions, and Nonprofits
pAM/CBA-eGFP-miRaSyn(human)x3-WPRE-bGHpA

Plasmid

#194247

Purpose

Expresses 3 miRNAs targeting human alpha-Synuclein

Depositor

Michael J Fox Foundation MJFF

Insert

miRaSynuclein(human) (SNCA Human)

Use

AAV and RNAi

Tags

Expression

Mutation

Promoter

CAG

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAM/SAR-CAG-aSyn(A53T)/HA-WPRE-bGHpA

Plasmid

#194242

Purpose

Expresses human alpha-Synuclein A53T

Depositor

Michael J Fox Foundation MJFF

Insert

aSynuclein(A53T) (SNCA Human)

Use

AAV

Tags

HA

Expression

Mutation

A53T

Promoter

SAR-CAG

Available since

Jan. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pJT410 AAV-Rapamycin Inducible-NFZ-Citrine with bGH pA

Plasmid

#202059

Purpose

Inducible AAV activator

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

AAV-Rapamycin Inducible-NFZ-Citrine with bGH pA

Use

AAV; Aav with reporter

Tags

Expression

Mammalian

Mutation

ITRs intact

Promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pAM-CAGGS-FLEX-H2B-3XHA-P2A-N2c(G)

Plasmid

#73477

Purpose

Encodes the G protein to enable transsynaptic RAV infection

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

H2B-3XHA-P2A-N2c(G)

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pAM-CAGGS-FLEX-H2B-GFP-P2A-N2c(G)

Plasmid

#73475

Purpose

Encodes the G protein to enable transsynaptic RAV infection

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

H2B-GFP-P2A-N2c(G)

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only
pAM/CBA-eGFP-miRaSyn(mouse)x3-WPRE-bGHpA

Plasmid

#194248

Purpose

Expresses 3 miRNAs targeting mouse alpha-Synuclein

Depositor

Michael J Fox Foundation MJFF

Insert

miRaSynuclein(mouse) (Snca Mouse)

Use

AAV and RNAi

Tags

Expression

Mutation

Promoter

CAG

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-PAM-sig-His6-gamma-PALcc-deltaGlyc

Plasmid

#25458

Depositor

Betty Eipper

Article

Chufan et al Structure. 2009 Jul 15. 17(7):965-73.

Insert

PAM (Pam Rat)

Use

Tags

His6 and PAM signal

Expression

Mammalian

Mutation

Contains aa 498-820 (PALcc= PAL catalytic core) …

Promoter

Available since

July 13, 2010

Availability

Academic Institutions and Nonprofits only
pAM316 pET28a-GGG-Titin[V15P]-23-K-9-C-14cyclinB-10GS-Y

Plasmid

#226349

Purpose

Expression of Titin V15P substrate with GS tail

Depositor

Andreas Martin

Article

Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

Insert

titin (TTN Human)

Use

Tags

Expression

Bacterial

Mutation

V15P

Promoter

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pJT360 AAV-Rapamycin Inducible-noActivator Domain -Citrine

Plasmid

#202058

Purpose

Inducible AAV with reporter gene and cloning site to add activator domains

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

AAV-Rapamycin Inducible-noActivator Domain -Citrine

Use

AAV; Aav with reporter

Tags

Expression

Mammalian

Mutation

ITRs intact

Promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-CMV-EGFP-PolB(K72A)-PAMmut-Hygro

Plasmid

#176087

Purpose

EGFP fused to the N-terminus of POLB containing the mutation Lys72Ala, a mutation in the PAM site used by POLBKOg1 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Mutation in Lys72 to Ala, and mutation in the PAM…

Promoter

CMV

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAMS823 His6-MBP-3C-ORF2p (238-1061, ORFeus-Hs) in pET41

Plasmid

#213024

Purpose

Expresses human LINE-1 ORF2p Core (238-1061) in E. coli as an N-MBP fusion

Depositor

Oliver Weichenrieder, Martin S. Taylor

Article

Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

Insert

LINE-1 ORF2p (238-1061)

Use

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

Promoter

T7 / Lac

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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