We narrowed to 9,947 results for: SUB

1. GFP-X3

   Plasmid

   #115568

   Purpose

   Synthetic acetylation substrate consisting of GFP fusion with 3 repeats of the Gcn5 consensus motif

   Depositor

   Michael Downey

   Article

   Rossl et al J Biol Chem. 2019 Apr 19;294(16):6227-62

   Insert

   Green Fluorescent Protein
   

   Expression

   Bacterial and Yeast

   Mutation

   Three repeats of the Gcn5 consensus motif are tag…

   Promoter

   ADH1

   Available Since

   Oct. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3_altPKA-Cα-mEGFP

   Plasmid

   #114132

   Purpose

   Alternative spliced variant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP.

   Depositor

   Haining Zhong

   Article

   Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

   Insert

   18747 (Prkaca Mouse)
   

   Expression

   Mammalian

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Cav2.2e[a10, 18a, Δ24a, 31a, 37b, 46]

   Plasmid

   #26568

   Depositor

   Diane Lipscombe

   Article

   Pan et al J Neurosci. 2000 Jul 1. 20(13):4769-75.

   Insert

   calcium channel alpha-1B subunit (Cacna1b Rat)
   

   Tags

   6XHis and V5

   Expression

   Mammalian

   Mutation

   contains exons a10, 18a, 31a, 37b, and 46. Del…

   Available Since

   Nov. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pCDNA3_shRNA resistant PKA-Cα-mEGFP

   Plasmid

   #114143

   Purpose

   Mammalian expression of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.

   Depositor

   Haining Zhong

   Article

   Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

   Insert

   mouse PKA-Cα-mEGFP with silent mutations resisting shRNA knockdown
   

   Expression

   Mammalian

   Available Since

   Oct. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ratiometric FPX sensor for ERK kinase activity

   Plasmid

   #60974

   Purpose

   For imaging of ERK kinase activity using a single polypeptide RA-WW domain-ERK substrate-B protein and free GA-NES.

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   ddRFP A-WW domain-ERK substrate-ddFP B
   

   Tags

   ERK substrate is before ddFP copy B, WW domain is…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pETDuet-msGC-NT21

   Plasmid

   #78102

   Purpose

   expresses the N-terminal fragment of manduca sexta soluble guanylyl cyclase subunits alpha and beta

   Depositor

   William Montfort

   Article

   Fritz et al Biochemistry. 2013 Mar 5;52(9):1568-82.

   Inserts

   fragment of alfa subunit of sGC
   fragment of beta subunit of sGC
   

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   fragment 1-380 of the beta subunit and fragment 2…

   Promoter

   T7

   Available Since

   June 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETDuet-msGC-NT13

   Plasmid

   #75087

   Purpose

   expresses the N-terminal fragment of manduca sexta soluble guanylyl cyclase subunits alpha and beta

   Depositor

   William Montfort

   Article

   Fritz et al Biochemistry. 2013 Mar 5;52(9):1568-82.

   Inserts

   fragment of alfa subunit of sGC
   fragment of beta subunit of sGC
   

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   fragment 1-380 of the beta subunit and fragment 4…

   Available Since

   May 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3_shRNA resistant nmPKA-Cα-mEGFP

   Plasmid

   #114144

   Purpose

   The G2A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.

   Depositor

   Haining Zhong

   Article

   Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

   Insert

   mouse PKA Cα-mEGFP with G2A mutation and silent mutation to resist shRNA knockdown (Prkaca Mouse)
   

   Expression

   Mammalian

   Mutation

   G2A mutation and silent mutation to resist shRNA …

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJK539

   Plasmid

   #72515

   Purpose

   Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit (AaPurL)

   Depositor

   T. J. Kappock

   Insert

   formylglycinamidine ribonucleotide synthetase, large subunit
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJK549

    Plasmid

    #72520

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit (AaPurL)

    Depositor

    T. J. Kappock

    Insert

    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJK555

    Plasmid

    #72521

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ)

    Depositor

    T. J. Kappock

    Insert

    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJK577

    Plasmid

    #72528

    Purpose

    Encodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS)

    Depositor

    T. J. Kappock

    Insert

    formylglycinamidine ribonucleotide synthetase, small subunit
    

    Use

    Gene synthesis

    Promoter

    n/a

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. MT4_C30Y R31W

    Plasmid

    #105712

    Purpose

    Bacterial expression plasmids for production of recombinant metallothionein 3 subisoform with C30Y and R31W substitution

    Depositor

    Artur Krężel

    Insert

    Metallothionein 4 (MT4 Human)
    

    Tags

    Chiting Binding Domanin-Intein

    Expression

    Bacterial

    Mutation

    This subisoform has a C30Y and R31W substitution

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJK532

    Plasmid

    #72513

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit with N-terminal His6 tag (H6AaPurQ)

    Depositor

    T. J. Kappock

    Insert

    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJK544

    Plasmid

    #72517

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS)

    Depositor

    T. J. Kappock

    Insert

    formylglycinamidine ribonucleotide synthetase, small subunit
    

    Expression

    Bacterial

    Mutation

    contains additional Ala2

    Promoter

    T7

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJK533

    Plasmid

    #72514

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS)

    Depositor

    T. J. Kappock

    Insert

    formylglycinamidine ribonucleotide synthetase, small subunit
    

    Expression

    Bacterial

    Mutation

    contains additional Ala2

    Promoter

    T7

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJK575

    Plasmid

    #72526

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit with C-terminal His6 tag (AaPurLH6)

    Depositor

    T. J. Kappock

    Insert

    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJK620

    Plasmid

    #72537

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit with N-terminal His6-tag (H6AaPurS)

    Depositor

    T. J. Kappock

    Insert

    formylglycinamidine ribonucleotide synthetase, small subunit
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJK622

    Plasmid

    #72538

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit with N-terminal His6-tag (H6AaPurL)

    Depositor

    T. J. Kappock

    Insert

    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAIP4_3TOW

    Plasmid

    #234201

    Purpose

    Heterologous protein expression of multivesicular body subunit 12B (ESCRT-I complex subunit MVB12B) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    3TOW (MVB12B Human)
    

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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