We narrowed to 4,839 results for: pcr
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Plasmid#67168PurposeCRISPaint universal donor for TagBFP fusion taggingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTagBFP
Available sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFA6a-A1-FLAG:hphMX4
Plasmid#134429PurposePCR-based C-terminal A1-FLAG tagDepositorInsertA1-FLAG
UsePcr-based tagging in yeastAvailable sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCR3.1-Luc
Plasmid#106457Purposeluciferase reporter, CMV promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertluciferase reporter, CMV promoter
UseLuciferaseExpressionMammalianMutationWT promoterAvailable sinceNov. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P31
Plasmid#120042PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with V5DepositorInsertV5
UsePcr templateTagsV5PromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR1063
Plasmid#111091PurposeExpresses dCas9-KRAB tagged with mCherry: pHR-hEF1a-dCas9-mcherry-KRAB-mPGK-HygDepositorInsertdCas9-KRAB
UseLentiviralTagsmCherryAvailable sinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRA03
Plasmid#103141PurposeTriple gRNA expression separated with 28nt Csy4 motifDepositorInsertgRNAs
ExpressionYeastAvailable sinceJan. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR_DMS3g24
Plasmid#89244PurposePlasmid encodes a synthetic CRISPR containing 7 copies of a single spacer, which targets a region (gene 24) in the genome of bacteriophage DMS3DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCRISPR
Available sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCR1055
Plasmid#71823PurposeExpress Streptococcus pyogenes nCas9 (H840A) nickase carrying two C-terminal SV40 NLSDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertT7pr_6xHis-MBP-TEV-SPynCas9 (H840A)-2xNLS
UseCRISPRAvailable sinceFeb. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCR1002
Plasmid#71820PurposeExpress Streptococcus pyogenes Cas9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertT7pr_10xHis-MBP-TEV-SPyCas9
UseCRISPRAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCRE-iRFP670
Plasmid#82696PurposeExpresses CRE and iRFP670 under two separate PGK promoters.DepositorInsertCRE, iRFP670
UseCre/LoxAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMaCTag-Z26
Plasmid#120069PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mAID-CloverDepositorInsertmAID-Clover
UsePcr templateTagsmAID-CloverPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTP-PCR C1
Plasmid#189618PurposeCloned Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteriaDepositorInsertMitochondrial Genome of Thalassiosira pseudonana
UseSynthetic Biology; Diatom expressionExpressionBacterial and YeastMutationMinor additional mutations described in manuscriptAvailable sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTP-PCR C4
Plasmid#189621PurposeCloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteriaDepositorInsertReduced Mitochondrial Genome of Thalassiosira pseudonana
UseSynthetic Biology; Diatom expressionExpressionBacterial and YeastMutationRepeat region removed, and minor additional mutat…Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTP-PCR C2
Plasmid#189619PurposeCloned Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteriaDepositorInsertMitochondrial Genome of Thalassiosira pseudonana
UseSynthetic Biology; Diatom expressionExpressionBacterial and YeastMutationMinor additional mutations described in manuscriptAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCR-Myt1NB580
Plasmid#22740DepositorInsertMyelin Transcription Factor 1 (Myt1 Mouse)
UsePcr cloning vectorAvailable sinceDec. 18, 2009AvailabilityAcademic Institutions and Nonprofits only -
IpO
Plasmid#48233PurposeBase vector for PCR-based DNA transplant in yeast. DNA segments to be transplanted into the yeast genome are cloned into the MCS.DepositorInsertsURA3 3' fragment
URA3 5' fragment
UseRoutine cloning vectorMutation-242 upstream to URA3 ORF +495 and URA3 ORF from …PromoterURA3 promoter and noneAvailable sinceFeb. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z28
Plasmid#120071PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 3xHADepositorInsert3xHA
UsePcr templateTags3xHAPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-12
Plasmid#119991PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with mScarletDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmScarlet
UsePcr templateTagsmScarletPromoterNoneAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR_eGFP_191
Plasmid#111821PurposeKnockout eGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA targeting eGFP 171-191
UseCRISPRTagsmCherryExpressionBacterial and MammalianPromoterU6Available sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcrRNA.con
Plasmid#61285PurposeConstitutive repeat-only E. coli CRISPR arrayDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterJ23119Available sinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by