We narrowed to 4,668 results for: pcr

1. pCR2.1 GFR alpha 2

   Plasmid

   #119899

   Purpose

   Mouse GFR alpha 2 In situ hybridization probe

   Depositor

   Wenqin Luo, David Ginty

   Article

   Luo et al Neuron. 2009 Dec 24;64(6):841-56. doi: 1

   Insert

   GDNF family receptor alpha-2 (Gfra2 Mouse)
   

   Use

   In situ hybridization probe

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Pcr4-12xMBS-PBS

   Plasmid

   #52984

   Purpose

   provide MBS-PBS alternating sequence

   Depositor

   Robert Singer

   Article

   Wu et al Sci Rep. 2014 Jan 9;4:3615. doi: 10.1038

   Insert

   12xMBS-PBS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   none

   Available since

   June 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCR3-FLAG-Ubc13-WT

   Plasmid

   #43938

   Depositor

   Bryant Darnay

   Article

   Lamothe et al J Biol Chem. 2007 Feb 9;282(6):4102-12.

   Insert

   ubiquitin-conjugating enzyme E2N (UBE2N Human)
   

   Use

   Tags

   2x FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPaint-TagGFP2-PuroR

   Plasmid

   #80970

   Purpose

   CRISPaint gene tagging

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Insert

   TagGFP2
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCre-SacB-zeo

   Plasmid

   #107706

   Purpose

   Expresses Cre recombinase for excising chromosomal loxP-Hyg-loxP cassette inserts.

   Depositor

   Kenan Murphy

   Article

   Murphy et al Methods Mol Biol. 2015;1285:177-99. doi:

   Insert

   ble (Zeo resistance)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPaint-2A-TurboGFP-PEST

   Plasmid

   #67180

   Purpose

   CRISPaint universal donor for gene expression quantification using destabilized TurboGFP

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Insert

   T2A-TurboGFP-PEST
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCre-P2A-mCherry

   Plasmid

   #212105

   Purpose

   Expression of Cre and mCherry in mammalian cells

   Depositor

   Daekee Lee

   Insert

   Cre recombinase and mCherry
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCR8/GW/TOPO BMI

   Plasmid

   #69796

   Purpose

   Gateway entry vector for BMI

   Depositor

   Gerard Cagney

   Insert

   BMI (BMI1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pCR8/GW/TOPO PCGF2

   Plasmid

   #69791

   Purpose

   Gateway entry vector for PCGF2

   Depositor

   Gerard Cagney

   Insert

   PCGF2 (PCGF2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pCRISPR-RFP entry

    Plasmid

    #192278

    Purpose

    Entry vector for cloning MmCas12m spacers. RFP flanked by MmCas12m CRISPR repeats can be digested out with BbsI.

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    RFP flanked by type V-M CRISPR repeat sequences
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23119

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRII-BbsI-sgRNAscaffold

    Plasmid

    #159352

    Purpose

    Expression vector for a customizable guide RNA

    Depositor

    Pim Pijnappel

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRISPR-Cas9-ScaligD

    Plasmid

    #125688

    Purpose

    Gene knockout/in for actinomycetes

    Depositor

    Tilmann Weber

    Article

    Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

    Insert

    streptomyces codon optimized spCas9, sgRNA casette, and ScaligD
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    ermE*/tipA

    Available since

    Nov. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRISPR-Cre stuffer v4

    Plasmid

    #158033

    Purpose

    lenti-viral construct with Cas9, Cre recombinase and U6 driven sgRNA casette with modified tracr RNA (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI)

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCRISPaint-HaloTag-PuroR

    Plasmid

    #80960

    Purpose

    CRISPaint gene tagging

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    HaloTag
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCRISPaint-TagRFP-PuroR

    Plasmid

    #80971

    Purpose

    CRISPaint gene tagging

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    TagRFP
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRII-1.1-1.10a

    Plasmid

    #69589

    Purpose

    OT-II TCR a chain cDNA in pCRII

    Depositor

    Francis R. Carbone

    Article

    Barnden et al Immunol Cell Biol. 1998 Feb;76(1):34-40.

    Insert

    TCR alpha clone 1.1 (Tcra Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pCR-Flag-IKKalpha

    Plasmid

    #15467

    Depositor

    Hiroyasu Nakano

    Article

    Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

    Insert

    IkB kinase alpha (Chuk Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRY2FL(deltaNLS)-mCherryN1

    Plasmid

    #26871

    Purpose

    Mammalian expression of cytoplasmic CRY2-mCherry

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    CRY2 (CRY2 Mustard Weed)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    NLS sequences deleted

    Promoter

    CMV

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCry2PHR-mCh-RhoA

    Plasmid

    #42958

    Purpose

    Expresses optogenetically controlled RhoA

    Depositor

    David Schaffer

    Article

    Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

    Insert

    Cry2PHR-mCherry-RhoA (RHOA Human, Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCRISPaint-3xP3-GFP

    Plasmid

    #130277

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes 3xP3-GFP visible eye marker and multiple cloning site.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    3xP3-GFP
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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