We narrowed to 9,226 results for: sacs

1. pBK1307-AAV-EFSNC-dSaCas9-HP1b(79-173)

   Plasmid

   #223152

   Purpose

   Expression of truncated HP1b with dSaCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   HP1b (CBX1 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 79-173

   Promoter

   EF1a

   Available since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBK2043-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9

   Plasmid

   #223163

   Purpose

   2nd gen. AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA Scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 204-310 (MECP2)

   Promoter

   Available since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-U6-msCAMK2d-sgRNA-cTNT-SaCas9-HA-miR122 TS

   Plasmid

   #209783

   Purpose

   Expresses SaCas9 by the specific cTNT promoter and sgRNA targeted the mouse Camk2d gene by U6 promoter, incorporation of the miR122 target sequences (miR122TS) into the 3’ untranslated region (3’ UTR)

   Depositor

   Yuxuan Guo

   Article

   Yang et al Circulation. 2024 May 28;149(22):1778-17

   Insert

   miR122 ts
   

   Use

   AAV

   Tags

   HA

   Expression

   Mutation

   Promoter

   cTNT

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CMV-FLEXfrt-SaCas9-U6-sgNtsr1(guide_2)

   Plasmid

   #205417

   Purpose

   Mutagenesis of Ntsr1, second guide

   Depositor

   Larry Zweifel

   Insert

   Ntsr1 (Ntsr1 Mouse)
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. NdeI – SacII – SpeI(NheI) - ANXA2 (29-339)

   Plasmid

   #136545

   Purpose

   Expresses ANXA2 chimeras in bacterial cells

   Depositor

   Laszlo Nyitray

   Article

   Ecsedi et al Structure. 2020 Aug 4;28(8):943-953.e4.

   Type

   Empty backbone

   Use

   Tags

   ANXA2 (29-339) and His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. LF901: pMAGIC (L1-R5) mU6::SaCas9 gRNA scaffold

   Plasmid

   #121811

   Purpose

   pMAGIC L1-R5 entry plasmid, contains empty mouse U6-driven SaCas9 gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCSD_SpCas9MT2-NLS-3xHA-NLS-SaCas9WT-2xNLS

   Plasmid

   #107321

   Purpose

   Expresses SpCas9 (R1333S) fused to SaCas9 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   SpCas9-SaCas9 fusion
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   SpCas9 (R1333S) is fused to SaCas9

   Promoter

   CMV IE94

   Available since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV:ITR-EF1aCore-SaCas9-dual(U6-sgRNA(backbone))-ITR

   Plasmid

   #207878

   Purpose

   AAV vector encoding EF1a core promoter-driven SaCas9 and two sgRNA cloning sites w/ the engineered Sa Guide scaffold variant (BbsI and PaqCI for sgRNA cloning).

   Depositor

   Yeh-Hsing Lao

   Article

   Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV:ITR-EF1aCore-SaCas9-single(U6-sgRNA(backbone))-ITR

   Plasmid

   #207880

   Purpose

   AAV vector encoding EF1a core promoter-driven SaCas9 and single sgRNA cloning site w/ the engineered Sa Guide scaffold variant (BbsI for sgRNA cloning).

   Depositor

   Yeh-Hsing Lao

   Article

   Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. PZac2.1 gfaABC1D-NLS-SaCas9-3xHA-NLS bGH

    Plasmid

    #178960

    Purpose

    Expresses saCas9 protein specifically in astrocytes

    Depositor

    Baljit Khakh

    Article

    Endo et al Science. 2022 Nov 4;378(6619):eadc9020.

    Insert

    Staphylococcus aureus Cas9 (SaCas9)
    

    Use

    AAV

    Tags

    3x HA

    Expression

    Mutation

    Promoter

    gfaABC1D (astrocyte specific)

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-SaCas9WT-2xNLS

    Plasmid

    #107320

    Purpose

    Expresses SpCas9 fused to SaCas9 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    SpCas9-SaCas9 fusion
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    SpCas9 is fused to SaCas9

    Promoter

    CMV IE94

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. 1255_pAAV-U6-SA-BbsI-MluI-gRNA-CB-SACas9-HA-OLLAS-spA

    Plasmid

    #109320

    Purpose

    Plasmid for AAV SaCas9 Mammalian Expression with a BbsI cloning site for a gRNA

    Depositor

    William Lagor

    Article

    Jarrett et al Arterioscler Thromb Vasc Biol. 2018 Jul

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. 1518_pAAV-U6-SA-eGFP-gRNA-HLP-SACas9-HA-OLLAS-spA

    Plasmid

    #109316

    Purpose

    Plasmid for liver-specific expression of AAV SaCas9 with a gRNA against eGFP

    Depositor

    William Lagor

    Article

    Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

    Insert

    eGFP gRNA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJEP317-pAAV-U6SaCas9gRNA(SapI)-EFS-GFP- KASH-pA

    Plasmid

    #113694

    Purpose

    U6 driven SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in gRNAs.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Inserts

    SaCas9 gRNA Cassete
    GFP
    

    Use

    AAV and CRISPR

    Tags

    KASH

    Expression

    Mutation

    Promoter

    Available since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-dSaCas9-2xNLS

    Plasmid

    #107319

    Purpose

    Expresses SpCas9 fused to dSaCas9 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    SpCas9-SaCas9 fusion
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    SpCas9 is fused to nuclease dead SaCas9 (D10A, N5…

    Promoter

    CMV IE94

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCSD_2xNLS-SpCas9MT3-NLS-3xHA-NLS-dSaCas9-2xNLS

    Plasmid

    #107318

    Purpose

    Expresses SpCas9 (R1335K) fused to dSaCas9 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    SpCas9-SaCas9 fusion
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    SpCas9 (R1335K) fused to nuclease dead SaCas9 (D1…

    Promoter

    CMV IE94

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. p231-pOsAct1::hpt-pZmUbi::BdPetC-p35S::mTurquoise

    Plasmid

    #129650

    Purpose

    Rieske FeS overexpression

    Depositor

    Susanne von Caemmerer

    Article

    Ermakova et al Commun Biol. 2019 Aug 16;2:314. doi: 10.

    Insert

    CDS of PetC gene encoding for the Rieske FeS subunit
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Codon modified for Golden Gate

    Promoter

    pZmUbi

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. 1375_pAAV-U6-SA-WTmLdlrEx14-gRNA2-N22-CB-SACas9-HA-OLLAS-spA

    Plasmid

    #109311

    Purpose

    Plasmid for AAV SaCas9 Mammalian Expression with a gRNA against Ldlr

    Depositor

    William Lagor

    Article

    Jarrett et al Arterioscler Thromb Vasc Biol. 2018 Jul

    Insert

    Ldlr gRNA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJEP310-pAAV-Mecp2P-SaCas9-P2A-HAFLAGHA-KASH-pA

    Plasmid

    #113687

    Purpose

    SaCas9 driven by the neuron specific promoter Mecp2P. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    SaCas9 (NEWENTRY )
    

    Use

    AAV and CRISPR

    Tags

    Flag, HAx2, KASH, and NLS

    Expression

    Mutation

    Promoter

    Cytomegalo Virus(CMV)

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJEP311-pAAV-EFS-SaCas9-P2A-HAFLAGHA-KASH-pA

    Plasmid

    #113688

    Purpose

    SaCas9 driven by EFS. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    SaCas9 (NEWENTRY )
    

    Use

    AAV and CRISPR

    Tags

    Flag, HAx2, KASH, and NLS

    Expression

    Mutation

    Promoter

    Cytomegalo Virus(CMV)

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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