We narrowed to 7,027 results for: CAR

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pGEX tensin-SH2

Plasmid

#46525

Depositor

Bruce Mayer

Publication

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

tensin (TNS1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1451-1588

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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XE129 beta2AR Fz1-pcDNA3.1

Plasmid

#16795

Depositor

Randall Moon

Publication

Liu et al Proc Natl Acad Sci U S A. 1999 Dec 7;96(

Insert

beta 2 AR Fz1 (Fzd1, Adrb2 Rat)

Use

Xenopus expression

Mutation

Chimera of beta 2 adrenergic receptor (extracellu…

Available since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
pMXS-IRES-BLAST flag-CAD T456A S1406A S1859A

Plasmid

#169826

Purpose

Expresses C-terminal flag-tagged CAD with mutation of reported S6K, PKA, and Erk1/2 phosphorylation site

Depositor

Richard Possemato

Publication

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

T456A S1406A S1859A; TCCC -> AGTC silent mutat…

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-GABRA1(A322D)-IRES2-EGFP

Plasmid

#170821

Purpose

human GABAA receptors alpha1 subunit carrying A322D mutation and IRES2-EGFP

Depositor

Tingwei Mu

Publication

Fu et al PLoS One. 2018 Nov 27;13(11):e0207948. d

Insert

Human GABAA receptor alpha1 subunit carrying A322D mutation (GABRA1 Human)

Tags

IRES2-EGFP

Expression

Mammalian

Mutation

change Alanine 322 to Aspartate

Available since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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6xHis-TEV-mCherry-LC3B-Gly (Microtubule-associated proteins 1A/1B light chain 3B)

Plasmid

#169168

Purpose

Conjugatable form of LC3B lacking 5 C-terminal amino acids (MKLSV), with C-terminal Glycine120 exposed for lipidation reaction.

Depositor

Sascha Martens

Publication

Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

Insert

MAP1LC3B (MAP1LC3B Human)

Tags

6X Histidine Tag, TEV cleavage site, mCherry

Expression

Bacterial

Promoter

T7 lac promoter

Available since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1-N-Myc_884-1102 USP7

Plasmid

#131249

Purpose

Mammalian expression of the USP7 UBL4-5 domains with an N-terminal Myc tag

Depositor

Roger Woodgate

Publication

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Tags

Myc

Expression

Mammalian

Mutation

Encodes USP7 UBL domains 4-5 (USP7 amino acids 88…

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-hAR-R617M,K618M,K632M,K633M

Plasmid

#115778

Purpose

Expresses human androgen receptor (R617M, K618M, K632M, K633M) in mammalian cells

Depositor

Elizabeth Wilson

Publication

Zhou et al J Biol Chem. 1994 May 6;269(18):13115-23

Insert

Androgen Receptor (AR Human)

Expression

Mammalian

Mutation

R617M, K618M ,K632M, K633M, insertion of Q81 and …

Promoter

CMV

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX RASGAP(C)-SH2

Plasmid

#46431

Depositor

Bruce Mayer

Publication

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Gap/RASGAP(C) (RASA1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 339-452

Promoter

Tac

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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p2Flag CMV2-ZO-2-N

Plasmid

#27418

Depositor

Marius Sudol

Publication

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Tags

2xFlag

Expression

Mammalian

Mutation

Deletion of the carboxy-terminus of ZO-2, so only…

Available since

April 12, 2011

Availability

Academic Institutions and Nonprofits only
pKE12-ISceI-fabp10a:loxP-BFP-loxP-Xla.Ctnnb1

Plasmid

#198240

Purpose

For I-SceI-mediated transgenesis in zebrafish; fabp10a promoter drives expression of activated β-catenin preceded by a blue fluorescent protein (BFP)-STOP cassette flanked by loxP sites

Depositor

Didier Stainier

Publication

Kalasekar et al Biol Open. 2019 Oct 17;8(10):bio047829.

Inserts

Zebrafish fabp10a promoter:floxedBFP, Xenopus pt-beta-catenin coding sequence, polyA (fabp10a Frog)
Zebrafish cryaa promoter:mcherry fluorescent protein coding sequence (cryaa Zebrafish)

Use

Zebrafish transgenesis

Available since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-Myc_535-888 USP7

Plasmid

#131248

Purpose

Mammalian expression of the USP7 UBL1-3 domains with an N-terminal Myc tag.

Depositor

Roger Woodgate

Publication

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Tags

Myc

Expression

Mammalian

Mutation

A mutant form of our pcDNA3.1-N-Myc_535-1102 USP7…

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pRD134

Plasmid

#132378

Purpose

contains the sequence of mScarlet for C-term fusions in the pGGD000 backbone (GreenGate)

Depositor

Rüdiger Simon

Publication

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

mScarlet

Use

Greengate entry plasmid

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pXY1-spc

Plasmid

#90488

Purpose

Plasmid with ptb promoter for gene expression in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052

Depositor

Sheng Yang

Publication

Zhang et al J Microbiol Methods. 2015 Jan;108:49-60.

Type

Empty backbone

Expression

Bacterial

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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NfPolyA_pET21b

Plasmid

#215413

Purpose

Expression construct for NfPolyA gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Publication

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NfPolyA

Expression

Bacterial

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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YCplac33-NAT

Plasmid

#140594

Purpose

Expression in S. cerevisiae

Depositor

David Sabatini

Publication

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Type

Empty backbone

Expression

Yeast

Available since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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MTK3_036

Plasmid

#123750

Purpose

Encodes mScarlet as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

mScarlet

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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SRpHi1

Plasmid

#102541

Purpose

Bacterial expression of EYFP fused to the cell penetrating peptide TAT with a GST tag for purification

Depositor

Stefan Hell

Publication

Richardson et al Nat Commun. 2017 Sep 18;8(1):577. doi: 1

Insert

Tat-EYFP

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pIMP1-Padc

Plasmid

#90486

Purpose

Plasmid with adc(CA_P0165) promoter for gene expression in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052

Depositor

Sheng Yang

Publication

Zhang et al J Microbiol Methods. 2015 Jan;108:49-60.

Type

Empty backbone

Expression

Bacterial

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pBAD-SKW

Plasmid

#190631

Purpose

Bacterial expression of genetically encoded potassium indicator SKW

Depositor

Robert Campbell

Publication

Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

Insert

SKW

Expression

Bacterial

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD-SPW

Plasmid

#190628

Purpose

Bacterial expression of genetically encoded protease indicator SPW

Depositor

Robert Campbell

Publication

Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

Insert

SPW

Expression

Bacterial

Available since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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