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pKJ318

Plasmid

#217495

Purpose

Mammlian expression of MmFnuc guide RNA

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

MmFnuc guide RNA scaffold

Expression

Mammalian

Available Since

April 11, 2024

Availability

Academic Institutions and Nonprofits only

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pME-QFGal4-SV40pA

Plasmid

#155118

Purpose

Middle entry vector containing QFGal4

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QFGal4

Use

Gateway middle entry vector

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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JD690

Plasmid

#160397

Purpose

Binary vector pGWB14 including citrus GRF4-GIF1 under 35S promoter/ Plant transformation

Depositor

Jorge Dubcovsky

Article

Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

Insert

35S::citrus GRF4-GIF1 chimera

Promoter

35S

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-Vti1b

Plasmid

#45914

Depositor

Noboru Mizushima

Article

Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

Insert

vesicle transport through interaction with t-SNAREs 1B (VTI1B Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only
pCS2-QFGal4-SV40pA

Plasmid

#155126

Purpose

In vitro transcription of QFGal4 mRNA

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QFGal4

Use

Expression in zebrafish

Expression

Mammalian

Promoter

SP6

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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T5-sgRNA

Plasmid

#113655

Purpose

sgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.

Depositor

Jeffrey Barrick

Article

Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

Insert

promoter T5 and sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

T5

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pNb4OMT-A53M

Plasmid

#216232

Purpose

Expresses a mutant Norbelladine 4'-O-Methyltransferase

Depositor

Simon d'Oelsnitz, Andrew Ellington

Article

d'Oelsnitz et al Nat Commun. 2024 Mar 7;15(1):2084. doi:

Insert

Nb4OMT-A53M

Use

Synthetic Biology

Mutation

A53M

Promoter

P150

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pME-QUAS5x:GFPNLS-SV40pA

Plasmid

#155115

Purpose

Middle entry vector containing QUAS5x element upstream of GFP-NLS.

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QUAS5x:GFPNLS

Use

Gateway middle entry vector

Promoter

QUAS5x-E1b

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti_DualsgRNA_CTRL_Puro_T2A_BFP2

Plasmid

#236730

Purpose

Dual sgRNA targeting CTRL expressing BFP with Puromycin selection

Depositor

Derek Narendra

Article

Thayer et al EMBO J. 2025 Nov 20. doi: 10.1038/s44318

Insert

Negative CTRL

Use

Lentiviral

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_DualsgRNA_CTRL_ER-dAPEX-BFP2

Plasmid

#236733

Purpose

Dual sgRNA targeting CTRL expressing dAPEX-BFP targeting to ER

Depositor

Derek Narendra

Article

Thayer et al EMBO J. 2025 Nov 20. doi: 10.1038/s44318

Insert

Negative CTRL

Use

CRISPR and Lentiviral

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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OA-1127B

Plasmid

#190997

Purpose

Expresses arrays of gRNA targeting rel1 promoter under U6b/c promoter

Depositor

Omar Akbari

Article

Bui et al PLoS Pathog. 2023 Jan 19;19(1):e1010842.

Insert

U6b:sgRNArel1A1 - U6c:sgRNArel1A2 - U6b:sgRNArel1A3 - U6c:sgRNArel1A4

Expression

Insect

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pME-QUASM1:GFPNLS-SV40pA

Plasmid

#155116

Purpose

Middle entry vector containing QUASM1 element upstream of GFP-NLS.

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QUASM1:GFPNLS

Use

Gateway middle entry vector

Promoter

QUASM1-E1b

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-YJR129c

Plasmid

#85118

Purpose

bacterial expression of YJR129c

Depositor

Pal Falnes

Article

Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

Insert

EFM3 (EFM3 Budding Yeast)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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hCRISPRi_dual_1_2

Pooled Library

#187246

Purpose

Ultra-compact, human genome-wide CRISPRi library targeting each gene with a single element encoding a dual sgRNA cassette.

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Species

Synthetic

Use

Lentiviral

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only
hCRISPRa_dual_1_2

Pooled Library

#187249

Purpose

Ultra-compact, human genome-wide CRISPRi library targeting each gene with a single element encoding a dual sgRNA cassette.

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Species

Synthetic

Use

Lentiviral

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only
hCRISPRi_dual_5_6

Pooled Library

#187248

Purpose

Ultra-compact, human genome-wide CRISPRi library targeting each gene with a single element encoding a dual sgRNA cassette.

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Species

Synthetic

Use

Lentiviral

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only
hCRISPRi_dual_3_4

Pooled Library

#187247

Purpose

Ultra-compact, human genome-wide CRISPRi library targeting each gene with a single element encoding a dual sgRNA cassette.

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Species

Synthetic

Use

Lentiviral

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only
hCRISPRa_dual_3_4

Pooled Library

#187250

Purpose

Ultra-compact, human genome-wide CRISPRi library targeting each gene with a single element encoding a dual sgRNA cassette.

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Species

Synthetic

Use

Lentiviral

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only
hCRISPRa_dual_5_6

Pooled Library

#187251

Purpose

Ultra-compact, human genome-wide CRISPRi library targeting each gene with a single element encoding a dual sgRNA cassette.

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Species

Synthetic

Use

Lentiviral

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only
pYES260-sc.eEF2

Plasmid

#85119

Purpose

yeast expression of EEF2

Depositor

Pal Falnes

Article

Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

Insert

EFT1/EFT2 (EFT2, EFT1 Budding Yeast)

Tags

6xHis

Expression

Yeast

Promoter

GAL1

Available Since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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