We narrowed to 4,880 results for: pir

1. pFUW-tetO-HOXB4

   Plasmid

   #139829

   Purpose

   doxycycline-inducible expression of Human HOXB4 in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

   Insert

   HOXB4 (HOXA6 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   R249P- Please see depositor comment below

   Promoter

   TRE-mCMV

   Available since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFUW-tetO-KLF4

   Plasmid

   #139828

   Purpose

   doxycycline-inducible expression of Human KLF4 in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

   Insert

   KLF4 (KLF4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE-mCMV

   Available since

   April 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-2/3-3'

   Plasmid

   #117325

   Purpose

   CRISPR-Cas9 for miR-124-2/3, 3'

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   gRNA miR-124-2/3-3' (MIR124-3 Synthetic)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-3-5'

   Plasmid

   #117323

   Purpose

   CRISPR-Cas9 for miR-124-3, 5'

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   gRNA miR-124-3-5' (MIR124-3 Synthetic)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. U6REST1_U6REST2.hPGK.BRN2.hPGK.Ascl1WPRE

   Plasmid

   #101852

   Purpose

   Direct reprogramming of adult fibroblasts to neurons

   Depositor

   Malin Parmar

   Article

   Drouin-Ouellet et al EMBO Mol Med. 2017 Aug;9(8):1117-1131. d

   Insert

   Ascl1, Brn2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6, PGK

   Available since

   Oct. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTMB352_ZFcharm_Kv1_split-inteins

   Plasmid

   #220848

   Purpose

   Lentiviral vector expressing ZFcharm Kv2 fused to ZF3 and MmZFP_81187 via split Npu inteins; perfect match ZF3 binding site located upstream of pEFS

   Depositor

   Jonathan Weissman

   Article

   Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

   Insert

   ZFcharm Kv2
   

   Use

   Lentiviral

   Tags

   P2A-mCherry

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   EFS

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV4-ApoE2

   Plasmid

   #87085

   Purpose

   Expresses ApoE2 in Mammalian cells

   Depositor

   Bradley Hyman

   Article

   Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

   Insert

   Apolipoprotein E2 (APOE Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTwist-SARS-CoV-2 Spike-deltaC18 JN.1

   Plasmid

   #216526

   Purpose

   Encodes SARS-CoV-2 spike for JN.1 pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike JN.1 (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   See Depositor Comments.

   Promoter

   CAG

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAS_4xMma PylT_FLAG-AbKRS-chIPYE

   Plasmid

   #140020

   Purpose

   Plasmid with 4xMmaPylT cassette and chimeric Mma/Mba PylRS for amber suppression and incorporation of photo-crosslinking-lysine; for transient or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   AbKRS-chIPYE (pylS Methanosarcina mazei and Methanosarcina bakeri)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   1-149 MbPylRS IPYE (V31I, T56P, H62Y, A100E) + 18…

   Promoter

   EF1

   Available since

   June 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. FLAG-PRMT3

    Plasmid

    #164695

    Purpose

    Expression of PRMT3 with an N-terminal FLAG tag

    Depositor

    Cheryl Arrowsmith

    Article

    Kaniskan et al Angew Chem Int Ed Engl. 2015 Apr 20;54(1

    Insert

    PRMT3 (PRMT3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTwist-SARS-CoV-2 Spike-deltaC18 KP.2

    Plasmid

    #223273

    Purpose

    Encodes SARS-CoV-2 spike KP.2 for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike KP.2 (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments.

    Promoter

    CAG

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTwist-SARS-CoV-2 Spike-deltaC18 LB.1

    Plasmid

    #223277

    Purpose

    Encodes SARS-CoV-2 spike LB.1 for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike LB.1 (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments.

    Promoter

    CAG

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO.1-REST_sh_seq1_WPRE

    Plasmid

    #127573

    Purpose

    shRNA to knock down RE1-silencing transcription factor (REST)

    Depositor

    Malin Parmar

    Article

    Drouin-Ouellet et al EMBO Mol Med. 2017 Aug;9(8):1117-1131. d

    Insert

    shRNA for knock down of REST (REST Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-tetON-GATA6-EF1a-TagRFP-2A-tet3G

    Plasmid

    #204189

    Purpose

    Doxycycline inducible lentiviral vector for human GATA6 overexpression

    Depositor

    Emma Rawlins

    Article

    Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

    Insert

    GATA6 (GATA6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetON

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV HA hRb delta CDK

    Plasmid

    #58906

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    all 15 Ser/Thr Cdk acceptor sites changed to Ala,…

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAG426GAL-SLEV_NS2B-GS-NS3

    Plasmid

    #203510

    Purpose

    Expresses SLEV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker

    Depositor

    Alejandro Chavez

    Article

    Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

    Insert

    poly SLEV NS2B-GS-NS3 (POLY )
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pInducer20 2xHA GPR153

    Plasmid

    #226812

    Purpose

    Tet-inducible lentiviral vector for 2xHA GPR153 expression

    Depositor

    Ramiro Iglesias-Bartolome

    Article

    Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

    Insert

    GPR153 (GPR153 Human)
    

    Use

    Lentiviral

    Tags

    2xHA tag followed by a GDPPVAT linker

    Expression

    Mutation

    Sequence optimized from NP_997253.2

    Promoter

    Available since

    May 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETM33_Nsp5_Mpro

    Plasmid

    #156475

    Purpose

    Bacterial expression of Sars-CoV2 Nsp5_Mpro protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Nsp5_Mpro (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pαH-S-GSAS/D614G

    Plasmid

    #164566

    Purpose

    SARS-CoV-2 Spike protein with D614G mutation (S-GSAS-D614G variant)

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

    Insert

    Spike (S-GSAS-D614G variant)
    

    Use

    Tags

    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pK18-hACE2

    Plasmid

    #149449

    Purpose

    Expresses human ACE2 driven by the K18 promoter

    Depositor

    Paul McCray

    Article

    McCray PB et al J Virol. 2007 Jan;81(2):813-21. doi: 10.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    K18

    Available since

    June 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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