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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,416 results for: sequence

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Showing: 3581 - 3600 of 13416 results

  1. MLM3636

    Plasmid
    #43860
    Purpose
    guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence, uses U6 promoter
    Depositor
    Keith Joung
    Insert
    None
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    March 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    43860-plasmid-map-sequence-id-194368

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  2. ORFtag_TetR-SD_Frame1

    Plasmid
    #220981
    Purpose
    ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame1
    Depositor
    Alexander Stark
    Insert
    TetR repressor
    Use
    Retroviral
    Expression
    Mammalian
    Promoter
    PGK promoter
    Available Since
    June 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    220981-plasmid-map-sequence-id-437540

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  3. AAV Ef1a-EGFP-WPRE

    Plasmid
    #135428
    Purpose
    Can be used to express EGFP. Can also be used to create adeno-associated virus for delivery of the EGFP sequence
    Depositor
    Rylan Larsen
    Insert
    EGFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Feb. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    135428-plasmid-map-sequence-id-271147

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  4. pLXRN-EGFP

    Plasmid
    #99207
    Purpose
    EGFP expressing bicistronic retroviral vector
    Depositor
    Peter Pedersen
    Insert
    EGFP
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    A Kozak sequence was incorporated to enhance tran…
    Promoter
    LTR
    Available Since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    99207-plasmid-map-sequence-id-190957

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  5. mTLR4 flag

    Plasmid
    #13087
    Depositor
    Ruslan Medzhitov
    Insert
    TLR4 (Tlr4 Mouse)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Amino acids 1-19 have been replaced with mammalia…
    Available Since
    Oct. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13087-plasmid-map-sequence-id-147760

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  6. Minos ITR loxp MCS lox2272

    Plasmid
    #218983
    Purpose
    Plasmid containing ITR sequences compatible with Minos transposase and multiple cloning site
    Depositor
    Amro Hamdoun
    Type
    Empty backbone
    Use
    Synthetic Biology
    Available Since
    Aug. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    218983-plasmid-map-sequence-id-436166

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  7. pAV hH1-ARRET

    Plasmid
    #111374
    Purpose
    ARRET expression contains approximately 130 anti-sense telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences
    Depositor
    Klaus Holzmann
    Insert
    ARRET w/o polyA
    Use
    Adenoviral
    Promoter
    hH1
    Available Since
    Sept. 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    111374-plasmid-map-sequence-id-209217

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  8. pJK407

    Plasmid
    #72421
    Purpose
    Produces Acetobacter aceti 1023 Est06, C-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    lipase
    Tags
    His6
    Expression
    Bacterial
    Mutation
    lacks Met1 and Thr2 in annotated (v1) sequence
    Promoter
    T7
    Available Since
    Feb. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    72421-plasmid-map-sequence-id-134675

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  9. pcDNA3+ hSlo2.2

    Plasmid
    #16214
    Depositor
    Larry Salkoff
    Insert
    KCNT1 potassium channel, subfamily (KCNT1 Human)
    Expression
    Mammalian
    Mutation
    This construct lacks the first 53aa when compared…
    Available Since
    Jan. 18, 2008
    Availability
    Academic Institutions and Nonprofits only
    16214-plasmid-map-sequence-id-248256

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  10. pcU6.3_gRNA_c1

    Plasmid
    #221381
    Purpose
    Chicken-specific U6 sgRNA expression mini-vector with 10x capture sequence 1
    Depositor
    James Briscoe, Tatjana Sauka-Spengler
    Type
    Empty backbone
    Use
    CRISPR
    Promoter
    G. gallus U6.3
    Available Since
    Nov. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    221381-plasmid-map-sequence-id-454411

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  11. pDual_dsCas9_Blast_hs_NC_chr1

    Plasmid
    #214690
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr1
    Use
    CRISPR and Lentiviral
    Available Since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214690-plasmid-map-sequence-id-428775

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  12. pDual_dsCas9_Blast_hs_TP73

    Plasmid
    #214691
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human TP73
    Depositor
    Kevin Janes
    Insert
    dgRNA_TP73 (TP73 Human)
    Use
    CRISPR and Lentiviral
    Available Since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214691-plasmid-map-sequence-id-428772

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  13. pET22B_PcrA_2Bchimera

    Plasmid
    #102998
    Purpose
    Express a DNA helicase, a chimeric construct of Bacilllus stearothermophilus PcrA and 2B domain of Staphylococcus aureus PcrA
    Depositor
    Martin Webb
    Insert
    PcrA_2Bchimera
    Expression
    Bacterial
    Mutation
    Bacilllus stearothermophilus PcrA in which the 2B…
    Promoter
    T7
    Available Since
    May 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    102998-plasmid-map-sequence-id-199442

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  14. pcDNA3.1-MS2-HA-HshnRNPE2iso3_Z

    Plasmid
    #148118
    Purpose
    Mammalian Expression of HshnRNPE2iso3
    Depositor
    Elisa Izaurralde
    Insert
    HshnRNPE2iso3 (PCBP2 Human)
    Expression
    Mammalian
    Mutation
    one non silent mutation L239 to S compapred to th…
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148118-plasmid-map-sequence-id-473831

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  15. pAd/CMV/PBX1-IRES-nEBFP2

    Plasmid
    #29755
    Depositor
    Edward McCabe
    Insert
    Pre-B-cell leukemia transcription factor 1 (PBX1 Human)
    Tags
    EBFP2, IRES, and Nuclear localization sequence
    Expression
    Mammalian
    Mutation
    No mutations; EBFP2 is nuclear localized; PBX1 an…
    Available Since
    Aug. 17, 2011
    Availability
    Academic Institutions and Nonprofits only

  16. N1-LOC8R

    Plasmid
    #66896
    Purpose
    Leader Of Cox8 Repeated (LOC8R) is a synthetic mitochondrial targeting sequence compatible with any N1 vector. Internal restriction sites enable modular subcloning of 1x, 2x, 3x or 4x presequences.
    Depositor
    Georg Ramm
    Insert
    LOC8R
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    66896-plasmid-map-sequence-id-114924

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  17. pET-24a-STE2

    Plasmid
    #133430
    Purpose
    Yeast STE2 coding sequence in vector for in vitro transcription and protein synthesis, with T7 promoter.
    Depositor
    Gerhard Wagner
    Insert
    STE2 (STE2 Budding Yeast)
    Expression
    Bacterial
    Mutation
    Both native Cys (C59S/C252A) have been mutated. S…
    Available Since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133430-plasmid-map-sequence-id-267723

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  18. pOX + hSlo2.2

    Plasmid
    #16215
    Depositor
    Larry Salkoff
    Insert
    KCNT1 potassium channel, subfamily (KCNT1 Human)
    Use
    Xenopus oocyte expression
    Mutation
    This construct lacks the first 53aa when compared…
    Available Since
    Jan. 18, 2008
    Availability
    Academic Institutions and Nonprofits only

  19. pENTR hH1-ARRET

    Plasmid
    #111382
    Purpose
    ARRET with approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences under control of hH1 promoter and terminator
    Depositor
    Klaus Holzmann
    Insert
    hH1-ARRET
    Use
    Gateway entry cloning
    Promoter
    hH1
    Available Since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    111382-plasmid-map-sequence-id-209222

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  20. PPI63

    Plasmid
    #25197
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ppi63.0007.0179 (NKTR Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 9, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 3581 - 3600 of 13416 results