We narrowed to 17,910 results for: REV
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Plasmid#192447PurposeNon-phosphorylatable negative-control mutant of ExRai-AMPKAR.DepositorInsertExRai-AMPKAR(T/A)
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tagExpressionMammalianMutationThr 6 in AMPK substrate mutated to Ala.PromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXD70CT5.1-PxylR-CymR, Pveg(2CuO)-LacZ
Plasmid#191628PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1 (tandem CymR binding sites)-LacZDepositorInsertlacZ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCfB2793
Plasmid#64043PurposepCfB2793 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.DepositorInsertGFP
UseCre/LoxTagsExpressionYeastMutationPromoterTEF1 promoter and noneAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPspCas13b-mNeongreen-NLS-3xFlag
Plasmid#132401Purposeoverexpression in human cellsDepositorInsertdPspCas13b
UseLentiviralTagsflagExpressionMammalianMutationH133A; H1059APromoterAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pK036.TRE-Flpe-WPRE (Supernova)
Plasmid#85007PurposeFor Flpe/FRT-based Supernova, pK036 should be used with pK068 etc.DepositorInsertNLS-FLPe-WPRE
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-LC3C WT
Plasmid#123095PurposeExpresses 3xFLAG-LC3C wild-type in mammalian cells.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-GCaMP6f-P2A-H2B-PAmCherry
Plasmid#133419PurposeFluorescent reporter for calcium imaging and photoactivationDepositorInsertGCaMP6f-P2A-H2B-PAmCherry (H2BC11 Human)
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
HypoxCR
Plasmid#59946PurposeA lentiviral dual fluorescent protein reporter, HypoxCR, detects hypoxic [hypoxia-inducible factor (HIF) active] and/or cycling cells.DepositorInsertsGFP-Pest
mCherry-Geminin
UseLentiviralTagsFLAG and PESTExpressionMammalianMutationPromoterCMV and min CMVAvailable sinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCfB2798
Plasmid#63659PurposepCfB2798 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.DepositorInsertGFP
UseCre/LoxTagsExpressionYeastMutationPromoterTEF1 promoterAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
p306-BrdU-Inc
Plasmid#71792PurposeBrdU-incorporating plasmid with selectable marker URA3DepositorInsertsUseTagsExpressionYeastMutationPromoterADH1 and GPDAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
MT363
Plasmid#65858PurposeThe gRNA construct is designed for constitutive expression of salicylate hydroxylase for degradation of salicylic acid to catechol.DepositorInsertnahG
UseTagsExpressionPlantMutationPromoterAvailable sinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
lenti-25xERSE2-minP-EGFP
Plasmid#159667PurposeEGFP reporter plasmid containing 25 tandem repeats of the ER stress response element-2 (ERSE2)DepositorInsert25xERSE2-minP-EGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
EC2_4_dCas9_VPR_sgRNA
Plasmid#163709PurposeYeast low copy plasmid with dCas9, sgRNA expression cassette and VPR activation domainsDepositorInsertsdCas9
VPR
UseTagsExpressionYeastMutationPoint mutations D10A and H840APromoterScTEF1Available sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
bNS2B47NS3
Plasmid#155320Purposeco-expression of DENV4 47 peptides from NS2B cofactor and NS3 full lengthDepositorInsertCo expression of DENV4 NS3 full length protein with 47 peptides from NS2B cofactor
UseTagsHis tagExpressionBacterialMutationWTPromoterT7Available sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pProEx-IDE-wt
Plasmid#99014PurposeExpress human Insulin Degrading Enzyme (IDE) in E. coli.DepositorInsertinsulin degrading enzyme (IDE Human)
UseTagsHis tagExpressionBacterialMutationPromoterTRCAvailable sinceAug. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRAB18-GFP
Plasmid#106984PurposeABA signaling reporter, GFP under the RAB18 promoterDepositorInsertsGFP
UseTagsExpressionPlantMutationPromoterRAB18Available sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBa.Halo-myosin Va tail 1006-1828
Plasmid#162715PurposeMammalian expression of rat WT Myosin Va with deletion of the 1005 N-terminal residuesDepositorInsertMyosin Va (Myo5a Rat)
UseTagsHaloExpressionMammalianMutationWT with deletion of the 1005 N-terminal residues.PromoterChicken Beta actinAvailable sinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-GFP ArgSen
Plasmid#184704PurposeLentiviral transfer plasmid for expression of GFP ArgSenDepositorInsertGFP ArgSen
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pS1643 (pFA6-NATMX-CDC42pr-yGFP1-10)
Plasmid#86420PurposeN-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoterDepositorInsertCDC42pr-GFP1-10
UseTagsGFP1-10ExpressionYeastMutationPromoterAvailable sinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKP112 (pRS406-GFP-ADH1t)
Plasmid#106472PurposeVector for creating C-terminal GFP fusion proteins in yeast.DepositorTypeEmpty backboneUseTagsGFPExpressionBacterial and YeastMutationPromoterNoneAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mas-GRK2RH-Nluc
Plasmid#158605PurposePlasmid expressing GRK2(RH)-Nluc in mammalian cellsDepositorInsertmyristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - GRK2 RH domain - linker (GGTGGS) - Nluc
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJT9R
Plasmid#98305PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of GAL1/GAL2 promoterDepositorInsertTRPL3AcNES1>TRPL41B
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV7.1_3xFLAG-WWC2
Plasmid#172995Purposeconstitutive expression of FLAG-tagged WWC2DepositorInsert3xFLAG-WWC2 (WWC2 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-myosin Vb tail 1436-1846
Plasmid#162714PurposeMammalian expression of rat WT Myosin Vb with deletion of the 1435 N-terminal residuesDepositorInsertMyosin Vb (Myo5b Rat)
UseTagsGFPExpressionMammalianMutationWT with deletion of the 1435 N-terminal residues.PromoterChicken Beta actinAvailable sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-GFP (F64L, S65T, V163A)-TRP1
Plasmid#53184Purposechromosomal tagging with stable GFPDepositorInsertTRP1 (TRP1 Budding Yeast)
UseYeast genomic targetingTagsGFP (F64L, S65T, V163A)ExpressionMutationPromoterAvailable sinceMay 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFlp-Km
Plasmid#222334Purposeflp-cl-sacB-pL1V-Lv1-neo-pBBR1-ELT3DepositorUseTagsExpressionMutationPromotercl, flp, and sacBAvailable sinceAug. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGal4x5-E4T-G210
Plasmid#171077PurposeThe resulting plasmid was used to generate a Gal4x5-E4-Gless 210bp construct for ampilifying the immobilized template for assays. Originally came from Michael Carey, was submitted with his permissionDepositorInsertGal4-E4-Gless-cassette
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
p405-BrdU-Inc
Plasmid#71791PurposeBrdU-incorporating plasmid with selectable marker LEU2DepositorInsertsUseTagsExpressionYeastMutationPromoterADH1 and GPDAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
YIP sHRPa-Aga1P
Plasmid#73151PurposeLarge fragment of split HRP fused to the N-terminus of the yeast mating protein Aga1P. When linearized, this vector incorporates into the yeast genome.DepositorInsertsHRPa-Aga1P
UseTagsV5 tagExpressionYeastMutationPromoterglyceraldehyde-3-phosphate dehydrogenase GPD prom…Available sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
lenti-25xAARE-minP-EGFP
Plasmid#159666PurposeEGFP reporter plasmid containing 25 tandem repeats of the amino acid response element (AARE)DepositorInsert25xAARE-minP-EGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLOX-CWBmi1
Plasmid#12240DepositorInsertBmi1 (Bmi1 Mouse)
UseCre/Lox and LentiviralTagsExpressionMammalianMutationCMV promoter upstream of mBmi-1. LoxP in 3' …PromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
beta-arrestin1 mYFP
Plasmid#36916DepositorInsertbeta-arrestin1 (Arrb1 Rat)
UseTagsmYFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
gRNA-trp-HYB
Plasmid#64331Purposetrp1 deletion gRNA cassette carried by pRS42HDepositorInsertgBlock product of trp1 deletion gRNA cassette
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
Nrxn3b-Nav1.6-MKII-f-hLOV1-TEVcs(ENLYFQ/M)-tTA-VP16
Plasmid#163031PurposeFLiCRE TF with fast kinetics for neuronal expressionDepositorInsertNrxn3b-MKII-f-hLOV1-TEVcs-tTA
UseAAVTagsExpressionMammalianMutationPromoterSynapsinAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-EGFP-RIP2
Plasmid#131201Purposemammalian expression, tet RDepositorInsertRIPK2 (RIPK2 Human)
UseTagsExpressionMammalianMutationPromoterpCMVAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV1 for Split-PE, SpCas9 nickase (PKC1001)
Plasmid#190111PurposeAAV1 for expression of nickase SpCas9(H840A) for Split-PE useDepositorInsertnSpCas9
UseAAVTagsbpNLSExpressionMutationH840APromoterEF1aAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLR5-CAG-TetR_W43F-3xmNG
Plasmid#174880PurposePiggyBac transposon vector for expression of TetR(W43F)-3x-mNeonGreenDepositorInsertTetR(W43F)-3xmNeonGreen
UsePiggybac vectorTagsExpressionMammalianMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
FLAG-OTUB1 C91S-pcDNA3.1
Plasmid#118210PurposeExpresses FLAG-OTUB1 C91S catalytic inactive mutant in mammalian cellsDepositorInsertOTUB1 (OTUB1 Human)
UseTags3x FLAGExpressionMammalianMutationchanged cysteine 91 to serinePromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCM184-ParB2::eGFP
Plasmid#87252PurposeExpresses ParB2-EGFP in yeast. Also known as pOR2-GFPDepositorInsertParB2
UseTagsEGFPExpressionYeastMutationPromoterpBAD/tetOAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1 puro GFP shRNA
Plasmid#10675PurposeNegative control vector for pMKO.1 puro (Addgene plasmid #8452); Contains shRNA against GFP.DepositorInsertGFP shRNA
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2006AvailabilityAcademic Institutions and Nonprofits only -
PAGFP_NS2B3
Plasmid#195755PurposeCloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.DepositorInsertDengue (Type-2) NS2B3
UseTagsPA-GFPExpressionMammalianMutationNo mutationsPromoterCMVAvailable sinceFeb. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 shSV40 neo
Plasmid#158646PurposeLentiviral shRNA vector targeting simian virus 40 T antigen (SV40Tag)DepositorInsertSV40Tag (SV40gp6 Simian virus 40)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 SA-2A-puro-pA_hSYN1_dTomato-SV40pA
Plasmid#196192Purposehuman synapsin promoter driven dTomato expression at human AAVS1 safe harbour locusDepositorInsert2xCHS4_hSYN1_INTRON_dTomato-WPRE-SV40-2xCHS4
UseZnf donorTagsExpressionMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2 Aga2P-HRP
Plasmid#73152PurposeFull-length horseradish peroxidase (HRP) fused to the C-terminus of the yeast mating protein Aga2P. Galactose-inducible expression.DepositorInsertAga2P-HRP
UseTagsHA tag and myc tagExpressionYeastMutationPromoterGAL1Available sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAT9679-sgRNA-BFP
Plasmid#162988PurposesgRNA cloning plasmidDepositorInsertexchangeable cassette
UseCRISPRTagsExpressionBacterial and MammalianMutationPromoterU6Available sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMB-SaCas9
Plasmid#105998PurposeSaCas9 expression vectorDepositorInsertCas9 nuclease
UseCRISPR and LentiviralTagsnoneExpressionMammalianMutationnonePromotershort EF1alphaAvailable sinceJuly 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCM189-ParB1::mCherry
Plasmid#87253PurposeExpresses ParB1-mCherry in yeast. Also known as pOR1-mchDepositorInsertParB1
UseTagsmCherryExpressionYeastMutationPromoterpBAD/tetOAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn FLEx-OFF hM4Di-mCherry
Plasmid#161579PurposeTo express hM4Di in cells not expressing Cre.DepositorInserthM4Di-mCherry
UseAAVTagsExpressionMutationNAPromoterhSynAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM7
Plasmid#154164PurposemCherry expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.DepositorInsertmCherry
UseTagsExpressionInsectMutationPromoterIE1 promoter and hr5 enhancer from Autographa cal…Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
plRL58
Plasmid#166886PurposeOptimized M. tuberculosis CRISPRi plasmid; contains an L5 attP region (no L5 Integrase) that allows for integration when co-transformed with an L5 Integrase delivery plasmid (e.g. plRL19, ID #163634)DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterTet-OnAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only