We narrowed to 9,927 results for: ef1;

1. pCDF1-ADAMTS18 (C638S)

   Plasmid

   #29555

   Depositor

   Yardena Samuels

   Article

   Wei et al Mol Cancer Res. 2010 Nov . 8(11):1513-25

   Insert

   ADAMTS18 (ADAMTS18 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed cysteine 638 to serine

   Promoter

   Available since

   July 22, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pCDF1-ADAMTS18 (G312E)

   Plasmid

   #29553

   Depositor

   Yardena Samuels

   Article

   Wei et al Mol Cancer Res. 2010 Nov . 8(11):1513-25

   Insert

   ADAMTS18 (ADAMTS18 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed glycine 312 to glutamic acid

   Promoter

   Available since

   July 22, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pJH104

   Plasmid

   #48262

   Purpose

   Template plasmid for PCR-based transplant of Saccharomyces cerevisiae TEF1 promoter in yeast.

   Depositor

   Ronald Davis

   Article

   Horecka et al Yeast. 2014 May;31(5):185-93. doi: 10.10

   Inserts

   TEF1p 5' fragment
   URA3 5' fragment
   TEF1p 3' fragment
   

   Use

   Routine cloning vector

   Tags

   Expression

   Mutation

   From SK1 background. -343 to -1 relative to TEF1 …

   Promoter

   TEF1p

   Available since

   Feb. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. EF.GFP

   Plasmid

   #17616

   Depositor

   Linzhao Cheng

   Article

   Cui et al Blood. 2002 Jan 15. 99(2):399-408.

   Insert

   EF-1a promoter (EEF1A1 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO5d.EFS.SpCas9.P2A.BSD

   Plasmid

   #57821

   Purpose

   Lentiviral Vector for SpCas9 Expression without sgRNA, Blasticidin resistance, EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   SpCas9
   EFS (EEF1A1 Human)
   P2A-BSD
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO5d.EFS.SpCas9.P2A.PAC

   Plasmid

   #58329

   Purpose

   Lentiviral Vector for SpCas9 Expression without sgRNA, Puromycin resistance, EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   SpCas9
   EFS (EEF1A1 Human)
   P2A-PAC
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. PHBA03

   Plasmid

   #83537

   Purpose

   Constitutive TEF1 promoter mediated expression of the UBiC, ARO4*, and TKL1 genes

   Depositor

   Claudia Vickers

   Article

   Williams et al Metab Eng Commun. 2016 May 11;3:142-152.

   Insert

   pTEF1-UBiC-CYC1t-pTEF1-ARO4*-CYC1t-pTEF1-TKL1-CYC1t
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pTEF1

   Available since

   Dec. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. PHBA02

   Plasmid

   #83536

   Purpose

   Constitutive TEF1 promoter mediated expression of the UBiC and ARO4* genes

   Depositor

   Claudia Vickers

   Article

   Williams et al Metab Eng Commun. 2016 May 11;3:142-152.

   Insert

   pTEF1-ARO4*-CYC1t-pTEF1-UBiC-CYC1t
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pTEF1

   Available since

   Dec. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. 512 pME-miR

   Plasmid

   #26032

   Depositor

   Nathan Lawson

   Article

   Nicoli et al Nature. 2010 Apr 22. 464(7292):1196-200.

   Insert

   ef1a (eef1a1l1 Zebrafish)
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 26, 2010

   Availability

   Academic Institutions and Nonprofits only

10. delCMV-mCherry

    Plasmid

    #214143

    Purpose

    Control sensor

    Depositor

    Leif Dehmelt

    Article

    Nanda et al Nat Commun. 2023 Dec 15;14(1):8356. doi:

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    delCMV

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. delCMV-mCitrine

    Plasmid

    #214144

    Purpose

    Volume marker / Control sensor

    Depositor

    Leif Dehmelt

    Article

    Nanda et al Nat Commun. 2023 Dec 15;14(1):8356. doi:

    Insert

    mCitrine
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    delCMV

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. delCMV-mCherry-2x-Rhotekin-GBD

    Plasmid

    #214142

    Purpose

    Rho activity sensor

    Depositor

    Leif Dehmelt

    Article

    Nanda et al Nat Commun. 2023 Dec 15;14(1):8356. doi:

    Insert

    3xRhotekin-GBD (Rtkn Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Only the GTPase binding domain (GBD) is included

    Promoter

    delCMV

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. delCMV-mCherry-3x-p67phox-GBD

    Plasmid

    #214141

    Purpose

    Rac activity sensor

    Depositor

    Leif Dehmelt

    Article

    Nanda et al Nat Commun. 2023 Dec 15;14(1):8356. doi:

    Insert

    3xp67phox-GBD (NCF2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Only the GTPase binding domain (GBD) is included

    Promoter

    delCMV

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. JPF0535a

    Plasmid

    #124042

    Purpose

    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing spdCas9 fused to tagBFP P2A tagBFP, and an sgRNA targeting pTRE expressing mAzamiGreen in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-spdCAS9::tagBFP-P2A-tagBFP-SV40PA_mu6-sgTRE_pTRE-NLS::mAzamiGreen-rgPA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. ARB365

    Plasmid

    #124048

    Purpose

    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to mRuby2 P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::mRuby2-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ARB366

    Plasmid

    #124049

    Purpose

    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to KRAB domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::KRAB-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ARB367

    Plasmid

    #124050

    Purpose

    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to VPR domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::VPR-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPL001

    Plasmid

    #184750

    Purpose

    Big-IN positive control payload encoding EF1a-driven GFP-T2A-BSD.

    Depositor

    Jef Boeke

    Article

    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

    Insert

    pEF1a-eGFP-T2A-BSD-bGHpA
    

    Use

    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPL001-BBTK

    Plasmid

    #184751

    Purpose

    Big-IN positive control payload encoding EF1a-driven GFP-T2A-BSD. Harbors a backbone counterselectable TK cassette.

    Depositor

    Jef Boeke

    Article

    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

    Insert

    pEF1a-eGFP-T2A-BSD-bGHpA
    

    Use

    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDH-puro-cMyc

    Plasmid

    #46970

    Purpose

    lentiviral expression of c-Myc

    Depositor

    Jialiang Wang

    Article

    Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-5

    Insert

    MYC (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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