We narrowed to 12,813 results for: otos

1. pJK048

   Plasmid

   #75230

   Purpose

   Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1-H45W) and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)

   Depositor

   T. J. Kappock, JoAnne Stubbe

   Article

   Hoskins et al Biochemistry. 2007 Mar 13;46(10):2842-55

   Inserts

   N5-carboxyaminoimidazole ribonucleotide mutase
   N5-carboxyaminoimidazole ribonucleotide synthetase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Pro64 to Arg; Asp65 to His; Gln206 to Arg and cha…

   Promoter

   T7 and n/a

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiPGK Blast DEST ERKKTRmRuby2

   Plasmid

   #90231

   Purpose

   Lentiviral vector to express ERK KTR mRuby2 under PGK promoter (With Blasticidin Resistance)

   Depositor

   Markus Covert

   Article

   Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

   Insert

   ERK Kinase Translocation Reporter
   

   Use

   Lentiviral

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   March 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTBK1

   Plasmid

   #87443

   Purpose

   express TBK1 in mammalian cells

   Depositor

   Georgios Stathopoulos

   Article

   Marazioti et al Nat Commun. 2018 Feb 14;9(1):672. doi: 1

   Insert

   TBK1 (Tbk1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmCTIP2-N106

   Plasmid

   #66808

   Purpose

   2nd generation lentiviral transfer plasmid. Expresses mouse CTIP2 with blasticidin resistance

   Depositor

   Andrew Yoo

   Article

   Richner et al Nat Protoc. 2015 Oct;10(10):1543-55. doi

   Insert

   CTIP2 isoform b (Bcl11b Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human EF1a

   Available since

   Sept. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. MYC-hTRAK2

   Plasmid

   #225142

   Purpose

   Expresses MYC-tagged human TRAK2

   Depositor

   Thomas Schwarz

   Article

   Davis et al Life Sci Alliance. 2022 Oct 27;6(1):e202

   Insert

   TRAK2 (TRAK2 Human)
   

   Use

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-Frankenbody-FLAG-mEGFP

   Plasmid

   #175293

   Purpose

   Track mature and nascent FLAG tagged proteins in live cells

   Depositor

   Tim Stasevich

   Article

   Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

   Insert

   anti-FLAG frankenbody fused with mEGFP
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-Frankenbody-FLAG-mRuby2

   Plasmid

   #175294

   Purpose

   Track mature and nascent FLAG tagged proteins in live cells

   Depositor

   Tim Stasevich

   Article

   Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

   Insert

   anti-FLAG frankenbody fused with mRuby2
   

   Use

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pEGFP-hGal3

   Plasmid

   #73080

   Purpose

   Expression in mammalian cells

   Depositor

   Tamotsu Yoshimori

   Article

   Maejima et al EMBO J. 2013 Aug 28;32(17):2336-47. doi:

   Insert

   Galectin-3 (LGALS3 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCXLE-hOCT3/4

   Plasmid

   #27076

   Purpose

   Non-integrating (episomal) expression of human OCT3/4

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Nat Methods. 2011 May;8(5):409-12

   Insert

   OCT3/4 (POU5F1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMJA285= pSin-TCRab-CMVp-Puro

    Plasmid

    #116875

    Purpose

    stable TCR expression in human T cells

    Depositor

    Marcos Alcocer

    Article

    Wang et al Biotechnol Lett. 2019 Oct;41(10):1095-11

    Insert

    TCR (TRAV10, TRBV25-1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    deletion of EF1-a promotor

    Promoter

    CMV Promotor

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-LC3

    Plasmid

    #21073

    Purpose

    Mammalian expression of rat LC3 fused to EGFP

    Depositor

    Tamotsu Yoshimori

    Article

    Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    First ATG is not included.

    Promoter

    CMV

    Available since

    May 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-Atg14L

    Plasmid

    #21635

    Depositor

    Tamotsu Yoshimori

    Article

    Matsunaga et al Nat Cell Biol. 2009 Apr . 11(4):385-96.

    Insert

    Atg14L (ATG14 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. lenti sgRNA(MS2)_puro optimized backbone

    Plasmid

    #73797

    Purpose

    optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and EF1A

    Available since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPV-Dual_promoter-EF1α-2xNLS-Cascade+Cas3-P (RD)

    Plasmid

    #204619

    Purpose

    Expression vector of Cascade and Cas3. Puromycin selectable.

    Depositor

    Akitsu Hotta

    Article

    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

    Insert

    Cas7, Cas5, Cas8, Cas11, Cas6, and Cas3
    

    Use

    CRISPR

    Tags

    Each protein has C- and N-terminal SV40 NLS

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMRXIP-GFP-Spo20(PABD)

    Plasmid

    #221758

    Purpose

    Stable expression of GFP-Spo20(PABD) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    Spo20 (SOS1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    tandem Spo20(PABD) (amino acids 422-551)

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. Integrin β3-GFP

    Plasmid

    #205091

    Purpose

    Express GFP-tagged human integrin β3 in mammalian cells

    Depositor

    Bianxiao Cui

    Article

    Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

    Insert

    Integrin beta 3 (ITGB3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-HRE-dUnaG

    Plasmid

    #124372

    Purpose

    UnaG fluorescent protein reporter for hypoxia-induced factor (HIF) signaling

    Depositor

    Roland Friedel

    Article

    Sattiraju et al Immunity. 2023 Jul 7:S1074-7613(23)00274

    Insert

    HRE-dUnaG
    

    Use

    Lentiviral

    Tags

    PEST-degron and Myc tag

    Expression

    Mutation

    Promoter

    HRE (HIF responsive element 5x + CMV minimal prom…

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAVS1-Tet-OsTIR1(F74G)-V5

    Plasmid

    #158664

    Purpose

    Tet-OsTIR1(F74G)-V5 donor for integration at the AAVS1 locus

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    Tet-OsTIR1(F74G) AAVS1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    F74G

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. Orai1-jGCaMP8f

    Plasmid

    #231630

    Purpose

    Green fluorescent reporter of Orai1-associated calcium influx

    Depositor

    Michael Cahalan

    Article

    Dynes et al Proc Natl Acad Sci U S A. 2023 Sep 26;12

    Insert

    Orai1-jGCaMP8f (ORAI1 Synthetic, Human)
    

    Use

    Tags

    jGCaMP8f

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Immediate Early

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pN1-PinkyCaMP

    Plasmid

    #232861

    Purpose

    A vector expressing the mScarlet based calcium indicator PinkyCaMP under CMV promoter

    Depositor

    Olivia Masseck

    Article

    Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

    Insert

    PinkyCaMP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMRXIB-mRuby3-LC3

    Plasmid

    #221738

    Purpose

    Stable expression of mRuby3-LC3 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    LC3B (Map1lc3b Rat)
    

    Use

    Retroviral

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3-EGFP-Cdc42-T17N

    Plasmid

    #12976

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    Cdc42 DN (CDC42 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    T17N Dominant negative

    Promoter

    CMV

    Available since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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23. pAAV-hSyn-OsTIR1(F74G)

    Plasmid

    #140730

    Purpose

    OsTIR1(F74G)-P2A-mAID-EGFP-NES

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    OsTIR1(F74G)-P2A-mAID-EGFP-NES
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. AAV-FLEX-Arch-GFP

    Plasmid

    #22222

    Purpose

    Cre-dependent viral expression of Arch-GFP for neuronal inhibition

    Depositor

    Edward Boyden

    Article

    Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

    Has Service

    AAV1 and AAV9

    Insert

    Arch
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    no

    Promoter

    CAG

    Available since

    Jan. 6, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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25. pcDNA3.1-CMV-CFP;UBC-Cre25nt

    Plasmid

    #65727

    Purpose

    Expresses Cre recombinase with a 25 nucleotide extracellular vesicle targeting sequence under the UBC promotor and CFP under the CMV promotor.

    Depositor

    Jacco van Rheenen

    Article

    Zomer et al Cell. 2015 May 21;161(5):1046-57. doi: 1

    Inserts

    CMV promoter driving CFP expression
    UBC promoter driving Cre-25nt expression
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and UBC

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Orai1-jGCaMP8f V203Y

    Plasmid

    #231631

    Purpose

    Green fluorescent reporter of Orai1-associated calcium influx

    Depositor

    Michael Cahalan

    Article

    Dynes et al Proc Natl Acad Sci U S A. 2023 Sep 26;12

    Insert

    Orai1-jGCaMP8f V203Y (ORAI1 Synthetic, Human)
    

    Use

    Tags

    jGCaMP8f V203Y

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Immediate Early

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEGFP-C2-MVP

    Plasmid

    #204543

    Purpose

    Expresses EGFP-tagged MVP in mammalian cells

    Depositor

    Masaaki Komatsu

    Article

    Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

    Insert

    MVP (MVP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV-mGold2t-Tubulin-C-18

    Plasmid

    #231779

    Purpose

    Visualization of tubulin in mammalian cells using mGold2t

    Depositor

    Francois St-Pierre

    Article

    Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

    Insert

    mGold2t-Tubulin-C-18 (TUBA1B Human)
    

    Use

    Tags

    mGold2s

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV_hSyn_WiChR_TS_mScarlet_ER

    Plasmid

    #205997

    Purpose

    High efficient, K+ selective channelrhodopsin for the inhibition of neurons

    Depositor

    Peter Hegemann, Johannes Vierock

    Article

    Vierock et al Sci Adv. 2022 Nov 16:eadd7729.

    Insert

    Wobblia Inhibitory Channelrhodopsin
    

    Use

    AAV

    Tags

    TS, mScarlet, ER

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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30. pEGFP Kindlin2

    Plasmid

    #105305

    Purpose

    To study effect of Kindlin 2 on Cell/ECM adhesion

    Depositor

    Kenneth Yamada

    Article

    Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

    Insert

    Kindlin2 (FERMT2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge