We narrowed to 4,052 results for: gast

1. pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XHA

   Plasmid

   #217511

   Purpose

   KD Recombinase dependent conditional smGdP-10XHA cassette

   Depositor

   S. Lawrence Zipursky

   Article

   Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

   Insert

   KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XHA
   

   Use

   Tags

   smGdP-10XHA

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR-myr-AKT-HA

   Plasmid

   #31790

   Depositor

   Xin Chen

   Article

   Calvisi et al Gastroenterology. 2011 Mar;140(3):1071-8

   Insert

   Akt1 (Akt1 Mouse)
   

   Use

   Entry vector

   Tags

   HA and Myr

   Expression

   Mutation

   Promoter

   Available since

   Oct. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pAWG-dCas9-VPR

   Plasmid

   #63802

   Purpose

   SP-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; drosophila vector

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

   Insert

   SP-dCas9-VPR
   

   Use

   CRISPR and Synthetic Biology

   Tags

   VPR (VP64-p65-Rta)

   Expression

   Insect

   Mutation

   Promoter

   act5c

   Available since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pDEST-HemmarG

   Plasmid

   #31221

   Depositor

   Yuh-Nung Jan

   Article

   Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

   Insert

   CD4-tdGFP (CD4 Human, Aequorea victoria, Fly)
   

   Use

   Tags

   CD4 and EGFP/GFP

   Expression

   Insect

   Mutation

   Fusion of signal peptide of Drosophila Akh gene, …

   Promoter

   Available since

   Oct. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUASTLOTattB_eGFP::Dpp

   Plasmid

   #163702

   Purpose

   GFP-tagged version of Dpp (Decapentaplegic) under control of UAS and LexO enhancers

   Depositor

   Markus Affolter

   Article

   Harmansa et al Nature. 2015 Nov 19;527(7578):317-22. do

   Insert

   decapentaplegic (dpp Fly)
   

   Use

   Cre/Lox

   Tags

   Tagged with eGFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Gal4-entry+NLS

   Plasmid

   #128013

   Purpose

   Vector containing the Gal4-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancer

   Depositor

   Julius Brennecke

   Article

   Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

   Insert

   Gal4 DBD-SV40NLS-3x-Flag
   

   Use

   Gal4 entry vector with nls to generate tethering …

   Tags

   Gal4-DBD-SV40NLS-3xFlag

   Expression

   Mutation

   Promoter

   Available since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAc5.1B-lambdaN-HA-DmeIF4G-trunc-V5His6_G

   Plasmid

   #146392

   Purpose

   Insect Expression of DmeIF4G-trunc. *Note: this plasmid does not contain an HA-tag as the name implies.

   Depositor

   Elisa Izaurralde

   Article

   Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

   Insert

   DmeIF4G-trunc (eIF4G1 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   322-1666 truncated version of elF4G sequence with…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR-E2F1

   Plasmid

   #37141

   Depositor

   Robert Duronio

   Article

   Shibutani et al Dev Cell. 2008 Dec;15(6):890-900.

   Insert

   E2F1 (E2f1 Fly)
   

   Use

   Gateway

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTIGER_mNeonGreen::3xFLAG::dCrk

   Plasmid

   #131137

   Purpose

   UASp construct for over-expression of Drosophila Crk with N-terminal monomeric NeonGreen and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Insert

   Crk (Crk Fly)
   

   Use

   Tags

   3XFLAG and mNeonGreen

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTIGER_tdTomato::3xFLAG::dCrk

    Plasmid

    #131138

    Purpose

    UASp construct for over-expression of Drosophila Crk with N-terminal tandem Tomato and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    Crk (Crk Fly)
    

    Use

    Tags

    3XFLAG and tdTomato

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. Pyr pUAST-HA

    Plasmid

    #69768

    Purpose

    Pyramus (FGF8-like-2) Ligand in P element-based pUAST vector for Gal4-regulated expression in Drosophila.

    Depositor

    Arno Muller

    Article

    Klingseisen et al Development. 2009 Jul;136(14):2393-402.

    Insert

    Pyramus (FGF8like-2) (pyr Fly)
    

    Use

    P element-based puast vector for gal4-regulated e…

    Tags

    HA-Tag

    Expression

    Insect

    Mutation

    241T amino acid insertion compared to reference s…

    Promoter

    hsp70 promoter

    Available since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pGEX-2T-TRR-C421-Y2404F

    Plasmid

    #182846

    Purpose

    mutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 2011-2431 aa). Expression in E. coli. by IPTG induction

    Depositor

    Peter Harte

    Article

    Tie et al Development. 2014 Mar;141(5):1129-39. do

    Insert

    TRR 2011-2431 (trr Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 20…

    Promoter

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. gRNAs[bTub+Sxl].1046B

    Plasmid

    #112692

    Purpose

    express two gRNA targeting bTub & Sxl under dU6-3 promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    U6.3-gRNA[bTub] and U6.3-gRNA[Sxl] (Sxl Fly)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. KHBD00297

    Plasmid

    #34423

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG10798 (Myc Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

15. KHBD00649

    Plasmid

    #39711

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG6172 (vnd Fly)
    

    Use

    Gateway donr vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pAc5.1B-EGFP-DmThor-V5His6

    Plasmid

    #79408

    Purpose

    Expresses EGFP-tagged and C-terminal V5His6-tagged DmThor in S2 cells

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

    Insert

    DmThor (Thor Fly)
    

    Use

    Schneider 2 cell expression

    Tags

    EGFP and V5-His6

    Expression

    Mutation

    Promoter

    Ac5 promoter

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. KHBD00517

    Plasmid

    #39618

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG5893 (D Fly)
    

    Use

    Gateway donr vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Sept. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

18. Hy_pMT Laccase2 MCS-HIPK3 Exon

    Plasmid

    #69888

    Purpose

    Expresses the human HIPK3 exon 2 circular RNA in Drosophila

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    HIPK3 (HIPK3 Human, Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Expresses aa1-194 of HIPK3

    Promoter

    Metallothionein Promoter (pMT)

    Available since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. KHBD00506

    Plasmid

    #39609

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG12196 (egg Fly)
    

    Use

    Gateway donr vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

20. KHBD00273

    Plasmid

    #34401

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG11987 (tgo Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only