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GW182 proteins cause PABP dissociation from silenced miRNA targets in the absence of deadenylation.
Zekri L, Kuzuoglu-Ozturk D, Izaurralde E
EMBO J. 2013 Apr 3;32(7):1052-65. doi: 10.1038/emboj.2013.44. Epub 2013 Mar 5.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
79258pAc5.1B-EGFP-DmeIF4EExpresses EGFP-tagged DmeIF4E in S2 cells
79259pAc5.1B-lambdaN-HA-DmeIF4G-V5His6Expresses lambdaN-HA-tagged and C-terminal V5-His6-tagged DmeIF4G in S2 cells
146188pAc5.1B-lambdaN-HA-DmeIF4E_EInsect Expression of DmeIF4E
147097pAc5.1B-lambdaN-DmPABPC1-siRNAres-HA_OInsect Expression of DmPABPC1-siRNAres
147333pAc5.1B-lambdaN-HA-HsTNRC6C_1207-1690-CIM1CIM2mut_QInsect Expression of HsTNRC6C_1207-1690-CIM1CIM2mut
147334pAc5.1B-lambdaN-HA-HsTNRC6C_1207-1690-CIM1CIM2mut-F1389A_QInsect Expression of HsTNRC6C_1207-1690-CIM1CIM2mut-F1389A
147335pAc5.1B-lambdaN-HA-HsTNRC6C_1207-1690-CIM1mut_QInsect Expression of HsTNRC6C_1207-1690-CIM1mut
147336pAc5.1B-lambdaN-HA-HsTNRC6C_1207-1690-CIM2mut_QInsect Expression of HsTNRC6C_1207-1690-CIM2mut
147337pAc5.1B-lambdaN-HA-HsTNRC6C-F1389A_QInsect Expression of HsTNRC6C-F1389A
147550pAc5.1B-EGFP-HsTNRC6C_1207-1609-M2WA_TInsect Expression of HsTNRC6C_1207-1609-M2WA
147551pAc5.1B-EGFP-HsTNRC6C_1207-1609-M2WA-CIM1-2mut_TInsect Expression of HsTNRC6C_1207-1609-M2WA-CIM1-2mut
147756pAc5.1B-MS2-DmPABPC1-V5His6_VInsect Expression of DmPABPC1
147757pAc5.1B-MS2-GST-V5His6_VInsect Expression vector
147758pAc5.1C-Fluc-5BoxB-6xbsMS2-HhR_VInsect Expression of 5BoxB-6xbsMS2-HhR
147759pAc5.1C-Fluc-5BoxB-PolyA-HhR_VInsect Expression of 5BoxB-PolyA-HhR
148385pAc5.1C-Fluc-A93-5BoxB-HhR_ACInsect Expression of A93-5BoxB-HhR

Antibodies from Article