We narrowed to 14,937 results for: pET

1. pET empty polycistronic destination vector (2E)

   Plasmid

   #29775

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET151-HIS-TEV-GST-PP-RPN13

   Plasmid

   #73741

   Purpose

   Expression of hRPN13 in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

   Insert

   RPN13 (ADRM1 Human)
   

   Use

   Tags

   6xHIS and GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

3. pET co-transformation cloning vector (13S-A)

   Plasmid

   #48323

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-45b-HOXD13(+7A)-IDR-mCherry

   Plasmid

   #145281

   Purpose

   Bacterial Expression of 6xHis HOXD13 (+7A) IDR mCherry Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   HOXD13(+7A)-IDR (HOXD13 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A-repeat expanded

   Promoter

   Available since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21-pelB-LaG-16-3xFLAG-LaG-2

   Plasmid

   #172765

   Purpose

   Bacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (3xFLAG linker)

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-16-3xFLAG-LaG-2
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. His-SUMO- flag-hDDI2-D252N_pET11a

   Plasmid

   #169123

   Purpose

   expression of protein

   Depositor

   Barbara Dirac-Svejstrup

   Article

   Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

   Insert

   hDDI (DDI2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D252N

   Promoter

   Available since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-mScarlet-TEV-Strep-His

   Plasmid

   #210823

   Purpose

   Bacterial overexpression vector for C-terminally Strep and His tagged mScarlet fluorescent protein

   Depositor

   Luke Chao

   Article

   Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

   Insert

   mScarlet
   

   Use

   Tags

   TwinStrep, 10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAM354 pET28a-GGG-Titin[V15P]-23-K-19-C-15

   Plasmid

   #226350

   Purpose

   Expression of proteasome substrate with tail for molecular ruler

   Depositor

   Andreas Martin

   Article

   Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

   Insert

   titin (TTN Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   V15P

   Promoter

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETM-60-NusA-TEV-HsRCK_296-472_E

   Plasmid

   #146209

   Purpose

   Bacterial Expression of HsRck_296-472. Contains a TEV site rather than a HRV 3C site downstream of NusA.

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

   Insert

   HsRck_296-472 (DDX6 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-Fgß-ddFLN4-HIS-ybbr

    Plasmid

    #101239

    Purpose

    wildtype Fgß peptide with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces

    Depositor

    Hermann Gaub

    Article

    Milles et al Science. 2018 Mar 30;359(6383):1527-1533

    Insert

    Fgß peptide, ddFLN4 fingerprint (FGB Human, D. discoideum)
    

    Use

    Tags

    HIS and ybbr

    Expression

    Bacterial

    Mutation

    C18S in ddFLN4

    Promoter

    T7

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-Duet1_6xHis-TEV-GABARAP-Gly

    Plasmid

    #190930

    Purpose

    Plasmid for the expression and purification of GABARAP. Internal Reference: SMC894

    Depositor

    Sascha Martens

    Article

    Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

    Insert

    GABARAP (GABARAP Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Ends with Glycine 116

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEW3: pET-32a Dot1L(2-416)

    Plasmid

    #124098

    Purpose

    Dot1L(2-416) expression plasmid

    Depositor

    Cynthia Wolberger

    Article

    Worden et al Cell. 2019 Feb 8. pii: S0092-8674(19)301

    Insert

    Dot1L(2-416) (DOT1L Human)
    

    Use

    Tags

    Trx-His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET15b/GgVcl 884-1066-ECFP

    Plasmid

    #46182

    Depositor

    Susan Craig

    Insert

    Vcl 884-1066 (VCL Chicken)
    

    Use

    Tags

    ECFP and His

    Expression

    Bacterial

    Mutation

    aa 884-1066 (tail domain)

    Promoter

    T7

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pET28a-His10-SHP1_PTPase (243-541aa)

    Plasmid

    #180812

    Purpose

    Expression of 10*His tagged human SHP1 phosphatase domain (243-541aa)

    Depositor

    Enfu Hui

    Article

    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    SHP1_PTPase (243-541aa) (PTPN6 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. HOXB8 (murine) HIS-tag pET

    Plasmid

    #8540

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXB8 (Hoxb8 Mouse)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    starts at amino acid #2 (Ser)

    Promoter

    Available since

    Aug. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pETNKI-his-SUMO-RNF168ring (1-189)

    Plasmid

    #63570

    Purpose

    Expression of human RNF168 ring domain (residues 1-189) tagged with His-SUMO

    Depositor

    Titia Sixma

    Article

    Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

    Insert

    RNF168 (RNF168 Human)
    

    Use

    Tags

    6xHis and SUMO3

    Expression

    Bacterial

    Mutation

    Ring domain only (residues 1-189)

    Promoter

    T7

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pET-His6-Sumo-MBP-CfaN

    Plasmid

    #126016

    Purpose

    Bacterial expression of MBP-CfaN fusion protein (with N-terminal His5-Sumo)

    Depositor

    Thomas Muir

    Insert

    His5-Sumo-MBP-CfaN
    

    Use

    Tags

    5xHis-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-28a-His6-SUMO-VAMP3

    Plasmid

    #135552

    Purpose

    Express rat VAMP3 in E. coli BL21, purified via HisPur Ni-NTA Resin

    Depositor

    Jingshi Shen

    Article

    Yu et al Methods Mol Biol. 2019;1860:237-249. doi

    Insert

    VAMP3
    

    Use

    Tags

    6xHis tag, thrombin recognition and cleavage site…

    Expression

    Bacterial

    Mutation

    No ATG start codon

    Promoter

    T7 promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET29b-eGFP-dspB(E184Q)-6xHis

    Plasmid

    #175783

    Purpose

    Plasmid for overexpression of recombinant His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

    Insert

    Dispersin B
    

    Use

    Tags

    Hexahistidine tag and eGFP

    Expression

    Bacterial

    Mutation

    E184Q

    Promoter

    T7

    Available since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET29b-mVenus-dspB(E184Q)-6xHis

    Plasmid

    #176576

    Purpose

    Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

    Insert

    Dispersin B
    

    Use

    Tags

    Hexahistidine tag and mVenus

    Expression

    Bacterial

    Mutation

    E184Q

    Promoter

    T7

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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