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MAC_N_KLF12

Plasmid

#167767

Purpose

Expresses KLF12 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

KLF12 (KLF12 Human)

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pFASTbac-HisGST-PTPIP51(36-470)

Plasmid

#170531

Purpose

Expresses Cleavable HisGST-tagged PTPIP51 (36-470) in Sf9

Depositor

Byung Il Lee

Article

Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

Insert

Protein tyrosine phosphatase interacting protein 51 (RMDN3 )

Tags

GST tag and His tag

Expression

Insect

Promoter

Polyhedrin

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-Miro1 (184-618)

Plasmid

#127626

Purpose

Human Miro1 lacking the first GTPase domain

Depositor

Josef Kittler

Article

Covill-Cooke et al EMBO Rep. 2020 Feb 5;21(2):e49865. doi:

Insert

Miro1 (RHOT1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

184-618 only

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pLB(N)CX-FLAG-CH3

Plasmid

#133718

Purpose

Retroviral expression vector driving CH3 domain fragment of human p300 (EP300), with N-terminal FLAG tag.

Depositor

James A. DeCaprio

Article

Borger et al J Virol. 2006 May . 80(9):4292-303.

Insert

EP300 (EP300 Human)

Use

Retroviral

Tags

Flag

Mutation

partial (not complete) p300 fragment, CH3 domain …

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pET21a - Ec-ClpX D144L

Plasmid

#111514

Purpose

Expresses E. coli ClpX with a C-terminal His tag and a mutation from D144 to L144

Depositor

Judy Lieberman

Article

Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

Insert

ClpX

Tags

His tag

Expression

Bacterial

Mutation

Aspartatic acid 144 to Leucine

Promoter

T7

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST14-SARA

Plasmid

#67389

Purpose

Expression of native hSara in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pRevLCav2FeS

Plasmid

#60808

Purpose

to disrupt iron sulfur cluster in yeast Rev3

Depositor

Youri Pavlov, Tahir Tahirov

Article

Baranovskiy et al J Biol Chem. 2012 May 18;287(21):17281-7

Insert

REV3 (REV3 Budding Yeast)

Expression

Yeast

Mutation

contains 647 C-terminal amino acids and 335 bp of…

Available Since

March 9, 2015

Availability

Academic Institutions and Nonprofits only
PRR4-bio-His

Plasmid

#52075

Purpose

Expresses full-length Proline-rich protein 4 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

PRR4 (PRR4 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pQTEV-C6orf60

Plasmid

#34813

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

C6orf60 (FAM184A Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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human CRKL transgenic construct

Plasmid

#38186

Depositor

Nora Heisterkamp

Article

Hemmeryckx et al Cancer Res. 2001 Feb 15;61(4):1398-405.

Insert

CRKL (CRKL Human)

Use

Mouse transgenic

Mutation

Contains human CRKL introns

Promoter

human CRKL

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
HOXB6-FLAGA-pSP65

Plasmid

#20994

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res. 1996 Mar 1;24(5):898-

Insert

HOXB6 (HOXB6 Human)

Use

In vitro trans/trans

Tags

FLAG

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pET30-GBF-E6

Plasmid

#21696

Depositor

James Baleja

Article

Liu et al J Mol Biol. 2009 Mar 6. 386(4):1123-37.

Insert

protein G domain B1 fused human papillomavirus type 16 amno acid residue 2-142 (E6 Human papillomavirus type 16)

Tags

Protein G B1 domain

Expression

Bacterial

Mutation

GB1 (1-56aa) fusion tag with mutations at three p…

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only
pTol2 hsp70l loxP-DsRed-loxP-nCas9n-GFP

Plasmid

#158962

Purpose

Cre-dependent Cas9-GFP expression

Depositor

Michael Brand

Article

Hans et al Nat Commun. 2021 Feb 18;12(1):1125. doi:

Inserts

hsp70l promoter
floxed DsRed cassette
Cas9-GFP

Use

CRISPR and Cre/Lox

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pmCh-N1 BLTP3A LIR mutant

Plasmid

#241249

Purpose

For exogenous expression of codon optimized BLTP3A (UHRF1BP1) lacking its LIR motif as a C-terminal fusion to RFP in mammalian cells

Depositor

Pietro De Camilli

Article

Hanna et al EMBO J (2025) 44: 6168 - 6195

Insert

BLTP3A (UHRF1BP1) Δ1129-1134 (BLTP3A Human)

Tags

RFP

Expression

Mammalian

Mutation

Δ1129-1134

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-FOXG1

Plasmid

#153124

Purpose

Expression of Flag tagged human FOXG1 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXG1 (FOXG1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-3FLAG-EEA1

Plasmid

#176491

Purpose

For lentiviral transduction of 3FLAG-EEA1

Depositor

Wade Harper

Article

Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

Insert

EEA1 (EEA1 Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-3XFLAG-hINO80

Plasmid

#44149

Depositor

Joan Conaway

Article

Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

Insert

hINO80 full length (INO80 Human)

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pPW3758 : CMVd3-HsIRE1-HaloTag

Plasmid

#185680

Purpose

Low-level transient expression of full-length HsIRE1 with a C-terminal HaloTag.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERN1 (ERN1 Synthetic, Human)

Expression

Mammalian

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pM-ABE

Plasmid

#196292

Purpose

35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding ABE in place of viral GP

Depositor

Zhenghe Li

Article

Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

Insert

Full length TSWV M antigenome encoding ABE in place of viral GP

Use

CRISPR

Tags

3×flag

Expression

Plant

Mutation

See depositor comments below

Promoter

duplicated cauliflower mosaic virus 35S promoter

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-Cre

Plasmid

#196140

Purpose

AAV-mediated expression of Cre under the CAG promoter .

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Cre

Use

AAV

Expression

Mammalian

Mutation

codon diversified

Promoter

CAG

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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